Physical Properties, Antibacterial and Antioxidant Properties of Raw South Africa Shea Butter against Samples from Libyan Market

Vigorous research has been conducted into the phenology of the shea tree, its usage and that of the shea butter extracted from the nuts of the shea fruit. Shea butter is the most valuable product in the Shea tree and its use as raw or in cosmetic and pharmaceutical products was expanded in the last years. The aim was to carry out comparative study between imported South African raw Shea Butter, one sample from Poland and samples from Libyan market on their physical properties (organoleptic), thin layer chromatography (TLC) chromatogram, phytochemical screening and diphenylpicrylhydrazil (DPPH) scavenging activity. As well as antimicrobial screening. Organoleptic test was carried out by comparing color and odor. TLC chromatogram was performed by spotting solution of samples in hexane on TLC plate and eluted twice in hexane: ethylacetate 8:2 to get good separation.  Phytochemical screening was performed to determine the presence of carbohydrates (Fehling test), steroids, and triterpens (Salkwiski test).  DPPH assay was carried out by spraying TLC sample spots by 0.2% DPPH methanolic solution. Antimicrobial test was conducted on Pseudomonas aeruginosa and Staphylococcus aureus. Mueller-Hinton agar was used with ciprofolxacin as +ve control and DMSO as–ve control.  As a result, Shea Butter samples showed wide diversity in color and odor which considered normal except two white samples that might undergo refining process, while spreadability and TLC chromatograms were similar. Samples and control showed presence of carbohydrates, steroids and triterpens. Control and samples had DPPH scavenging activity. Shea Butter control and samples had no antimicrobial activities against P. aeruginosa and S. aureus.

Experimental study comparing burn healing effects of raw South African Shea butter and the samples from a Libyan market

Background: The fat extracted from the nut of the African Shea tree (Vitellaria paradoxa) is called Shea butter. It has multiple uses at the local level as it is used in cosmetic products and as a cocoa butter substitute in chocolate industries. It has a high nutritious value and is also a valuable product on the local, national, and international markets, making it the ideal candidate to research and invest in. Aim: This study is a comparative experimental study of the possible burn healing effects between imported South African raw Shea butter and samples in a Libyan market. Method: The control samples were brought from South Africa (Benin traditional markets). A total of 18 different samples were collected from different sale centers in Tripoli, including pharmacies, beauty shops, and spices shops, in addition to one sample brought from Poland. Animal experiment on burn healing effect was carried out on nine male Sprague Dawley (350–400 g) rats aged 6–8 weeks old. After shaving the animal’s dorsum hair, a metal cube was used to create a deep second degree burn wound, and the cube was heated to 100°C for 20 seconds. Medication with Shea butter (control, T1, and T2) was initiated daily for one for these groups by the application of a thin film of the Shea butter samples on the burned areas. On days 1, 3, and 7, the rats were anesthetised and a sample from the burned scar tissue and skin adjacent were evaluated using pathological parameters. Results: The histological study indicates that the use of Shea butter T1 as topical treatment induces an immune response, which enhances the form of the presence of a large number of inflammatory cells in the epidermis and dermis layers. The treatment of burned skin with T2 lasted for 72 hours and it showed slightly significant healing in the normal structure of proliferative granulation tissue with accumulation of fibroblasts and inflammatory cells surrounding the sebaceous glands and hair follicles. Small areas of the epidermis which formed few layers were observed and some hair roots were grown. This was well seen in cases of T1 and T2. Shea butter bought as raw might have a bad effect on burned skin. Conclusion: Shea butter bought as raw might have bad effect on burned skin. On the other hand, the sample from Poland had a therapeutic effect, which was because of the additives such as avocado oil, grape seed oil, and others

A population study of clinically actionable genetic variation affecting drug response from the Middle East

Clinical implementation of pharmacogenomics will help in personalizing drug prescriptions and alleviate the personal and financial burden due to inefficacy and adverse reactions to drugs. However, such implementation is lagging in many parts of the world, including the Middle East, mainly due to the lack of data on the distribution of actionable pharmacogenomic variation in these ethnicities. We analyzed 6,045 whole genomes from the Qatari population for the distribution of allele frequencies of 2,629 variants in 1,026 genes known to affect 559 drugs or classes of drugs. We also performed a focused analysis of genotypes or diplotypes of 15 genes affecting 46 drugs, which have guidelines for clinical implementation and predicted their phenotypic impact. The allele frequencies of 1,320 variants in 703 genes affecting 299 drugs or class of drugs were significantly different between the Qatari population and other world populations. On average, Qataris carry 3.6 actionable genotypes/diplotypes, affecting 13 drugs with guidelines for clinical implementation, and 99.5% of the individuals had at least one clinically actionable genotype/diplotype. Increased risk of simvastatin-induced myopathy could be predicted in ~32% of Qataris from the diplotypes of SLCO1B1, which is higher compared to many other populations, while fewer Qataris may need tacrolimus dosage adjustments for achieving immunosuppression based on the CYP3A5 diplotypes compared to other world populations. Distinct distribution of actionable pharmacogenomic variation was also observed among the Qatari subpopulations. Our comprehensive study of the distribution of actionable genetic variation affecting drugs in a Middle Eastern population has potential implications for preemptive pharmacogenomic implementation in the region and beyond. 2022, The Author(s).PVJ is supported by faculty funding from the College of Health & Life Sciences, HBKU. Qatar Biobank and Qatar Genome Program are Research, Development & Innovation's entities within Qatar Foundation for Education, Science and Community Development. Funders had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.Scopu