Integrated assessment of the environmental hazard level of technologies of drilling and blasting operations with using emulsion explosives in quarries

Purpose. Adaptation of the “unified method of quantitative and qualitative assessment of the level of environmental hazard of the operation of industrial enterprises, facilities and introduced technologies in the field of production and ecology” to the technologies of drilling and blasting operations in quarries for extraction of ore and nonmetallic materials with the use of various types of explosives. The methods. They are based on a heuristic evaluation of the impact of priority man-made factors of the technologies used according to the 4-point scale (0, 1, 2, 3), and then – of the complex level of their environmental hazard – according to the total 15-point scale, evenly divided into three qual-itative levels. Findings. The identification of types and sources of environmental hazard for natural environments (atmosphere, hydrosphere, lithosphere, soils and biota) was performed when using emulsion and TNT-containing explosives during drilling and blasting operations in quarries. The intensity, perio-dicity and consequences of various accompanying man-made factors were taken as the criteria of influence of dangerous impact of technological processes on the condition of the above natural en-vironments. It was found that the integrated level of the environmental hazard of man-made impacts of drilling and blasting operations using emulsion explosives was 2.33, in other words, it was “low”. When using TNT-containing explosives it was 5.00, i.e. appeared on the boundary of the first and the second ranges, where the “low” hazard level changes to “moderate”. This is an evi-dence of a higher (more than 2 times) environmental efficiency of using emulsion explosives com-pared to the use of TNT-containing explosives. The originality. The system of identification and management of environmental and technology-related risks under conditions of extraction of ore and nonmetallic materials in quarries by the drill and blast method was proposed, whereupon an integrated assessment of the level of environmental hazard of mining enterprises is proposed to be defined as a sum of average grade points (for each of the man-made factors) according to quantitative-qualitative scale. Practical implications. The developed methods allow determining with a sufficient degree of reli-ability the level of environmental hazard of existing technologies of drilling and blasting operations and substantiating expediency of change-over to emulsion explosive materials

Elementary derivation of Spitzer's asymptotic law for Brownian windings and some of its physical applications

A simple derivation of Spitzer'z asymptotic law for Brownian windings [Trans.Am.Math.Soc.87,187 (1958)]is presented along with its generalizations >.These include the cases of planar Brownian walks interacting with a single puncture and Brownian walks on a single truncated cone with variable conical angle interacting with the truncated conical tip.Such situations are typical in the theories of quantum Hall effect and 2+1 quantum gravity, respectively .They also have some applications in polymer physic

Veneziano Amplitudes, Spin Chains and String Models

In a series of recently published papers we reanalyzed the existing treatments of Veneziano and Veneziano-like amplitudes and the models associated with these amplitudes. In this work we demonstrate that the already obtained new partition function for these amplitudes can be exactly mapped into that for the Polychronakos-Frahm (P-F) spin chain model. This observation allows us to recover many of the existing string-theoretic models, including the most recent ones.Comment: 38 page

Signaling Cascades Modulate the Speed of Signal Propagation through Space

Cells are not mixed bags of signaling molecules. As a consequence, signals must travel from their origin to distal locations. Much is understood about the purely diffusive propagation of signals through space. Many signals, however, propagate via signaling cascades. Here, we show that, depending on their kinetics, cascades speed up or slow down the propagation of signals through space, relative to pure diffusion.We modeled simple cascades operating under different limits of Michaelis-Menten kinetics using deterministic reaction-diffusion equations. Cascades operating far from enzyme saturation speed up signal propagation; the second mobile species moves more quickly than the first through space, on average. The enhanced speed is due to more efficient serial activation of a downstream signaling module (by the signaling molecule immediately upstream in the cascade) at points distal from the signaling origin, compared to locations closer to the source. Conversely, cascades operating under saturated kinetics, which exhibit zero-order ultrasensitivity, can slow down signals, ultimately localizing them to regions around the origin.Signal speed modulation may be a fundamental function of cascades, affecting the ability of signals to penetrate within a cell, to cross-react with other signals, and to activate distant targets. In particular, enhanced speeds provide a way to increase signal penetration into a cell without needing to flood the cell with large numbers of active signaling molecules; conversely, diminished speeds in zero-order ultrasensitive cascades facilitate strong, but localized, signaling

Entropically driven transition to a liquid-crystalline polymer globule

A self-consistent-field theory (SCFT) in the grand canonical ensemble formulation is used to study transitions in a helix-coil multiblock copolymer globule. The helices are modeled as stiff rods. In addition to the established coil-globule transition we show for the first time that, even without explicit rod-rod alignment interaction, the system undergoes a transition to a nematic liquid-crystalline (LC) globular state. The LC-globule formation is driven by the hydrophobic helical segment attraction and the anisotropy of the globule surface energy. The full phase diagram of the copolymer was calculated. It discriminates between an open chain, amorphous globule and LC-globule. This model provides a relatively simple example of the interplay between secondary and tertiary structures in homopolypeptides. Moreover, it gives a simple explanation for the formation of helix bundles in certain globular proteins.Comment: 5 pages, 5 figures, submitted to Europhys. Let

Field theoretical representation of the Hohenberg-Kohn free energy for fluids

To go beyond Gaussian approximation to the Hohenberg-Kohn free energy playing the key role in the density functional theory (DFT), the density functional \textit{integral} representation would be relevant, because field theoretical approach to perturbative calculations becomes available. Then the present letter first derives the associated Hamiltonian of density functional, explicitly including logarithmic entropy term, from the grand partition function expressed by configurational integrals. Moreover, two things are done so that the efficiency of the obtained form may be revealed: to demonstrate that this representation facilitates the field theoretical treatment of the perturbative calculation, and further to compare our perturbative formulation with that of the DFT.Comment: 5 pages, revtex, modified on 13 April 2000 [see eqs. (3), (6), and (13)

Probing the Heterogeneity of Protein Kinase Activation in Cells by Super-Resolution Microscopy

Heterogeneity of mitogen-activated protein kinase (MAPK) activation in genetically identical cells, which occurs in response to epidermal growth factor receptor (EGFR) signaling, remains poorly understood. MAPK cascades integrate signals emanating from different EGFR spatial locations, including the plasma membrane and endocytic compartment. We previously hypothesized that in EGF-stimulated cells the MAPK phosphorylation (pMAPK) level and activity are largely determined by the spatial organization of the EGFR clusters within the cell. For experimental testing of this hypothesis, we used super-resolution microscopy to define EGFR clusters by receptor numbers (N) and average intra-cluster distances (d). From this data, we predicted the extent of pMAPK with 85% accuracy on a cell-to-cell basis with control data returning 54% accuracy (P50nm were most predictive for pMAPK level in cells. Electron microscopy revealed that these large clusters were primarily localized to the limiting membrane of multivesicular bodies (MVB). Many tighter packed dimers/multimers (d<50nm) were found on intraluminal vesicles within MVBs, where they were unlikely to activate MAPK because of the physical separation. Our results suggest that cell-to-cell differences in N and d contain crucial information to predict EGFR-activated cellular pMAPK levels and explain pMAPK heterogeneity in isogenic cells

Self- generated disorder and structural glass formation in homopolymer globules

We have investigated the interrelation between the spin glasses and the structural glasses. Spin glasses in this case are random magnets without reflection symmetry (e.g. $p$ - spin interaction spin glasses and Potts glasses) which contain quenched disorder, whereas the structural glasses are here exemplified by the homopolymeric globule, which can be viewed as a liquid of connected molecules on nano scales. It is argued that the homopolymeric globule problem can be mapped onto a disorder field theoretical model whose effective Hamiltonian resembles the corresponding one for the spin glass model. In this sense the disorder in the globule is self - generated (in contrast to spin glasses) and can be related with competitive interactions (virial coefficients of different signs) and the chain connectivity. The work is aimed at giving a quantitative description of this analogy. We have investigated the phase diagram of the homopolymeric globule where the transition line from the liquid to glassy globule is treated in terms of the replica symmetry breaking paradigm. The configurational entropy temperature dependence is also discussed.Comment: 22 pages, 4 figures, submitted to Phys. Rev.

A Hidden Feedback in Signaling Cascades Is Revealed

Cycles involving covalent modification of proteins are key components of the intracellular signaling machinery. Each cycle is comprised of two interconvertable forms of a particular protein. A classic signaling pathway is structured by a chain or cascade of basic cycle units in such a way that the activated protein in one cycle promotes the activation of the next protein in the chain, and so on. Starting from a mechanistic kinetic description and using a careful perturbation analysis, we have derived, to our knowledge for the first time, a consistent approximation of the chain with one variable per cycle. The model we derive is distinct from the one that has been in use in the literature for several years, which is a phenomenological extension of the Goldbeter-Koshland biochemical switch. Even though much has been done regarding the mathematical modeling of these systems, our contribution fills a gap between existing models and, in doing so, we have unveiled critical new properties of this type of signaling cascades. A key feature of our new model is that a negative feedback emerges naturally, exerted between each cycle and its predecessor. Due to this negative feedback, the system displays damped temporal oscillations under constant stimulation and, most important, propagates perturbations both forwards and backwards. This last attribute challenges the widespread notion of unidirectionality in signaling cascades. Concrete examples of applications to MAPK cascades are discussed. All these properties are shared by the complete mechanistic description and our simplified model, but not by previously derived phenomenological models of signaling cascades

Pathwise Sensitivity Analysis in Transient Regimes

The instantaneous relative entropy (IRE) and the corresponding instanta- neous Fisher information matrix (IFIM) for transient stochastic processes are pre- sented in this paper. These novel tools for sensitivity analysis of stochastic models serve as an extension of the well known relative entropy rate (RER) and the corre- sponding Fisher information matrix (FIM) that apply to stationary processes. Three cases are studied here, discrete-time Markov chains, continuous-time Markov chains and stochastic differential equations. A biological reaction network is presented as a demonstration numerical example