Piezoelectric Phononic Plates: Retrieving the Frequency Band Structure via All-electric Experiments

We propose an experimental technique based on all-electric measurements to retrieve the frequency response of a one-dimensional piezoelectric phononic crystal plate, structured periodically with millimeter-scaled metallic strips on its two surfaces. The metallic electrodes, used for the excitation of Lamb-like guided modes in the plate, ensure at the same time control of their dispersion by means of externally loaded electric circuits that offer non-destructive tunability in the frequency response of these structures. Our results, in very good agreement with finite-element numerical predictions, reveal interesting symmetry aspects that are employed to analyze the frequency band structure of such crystals. More importantly, Lamb-like guided modes interact with electric-resonant bands induced by inductance loads on the plate, whose form and symmetry are discussed and analyzed in depth, showing unprecedented dispersion characteristics.Comment: This is the version of the article before peer review or editing, as submitted by an author to Smart Materials and Structures. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1361-665X/ab4aa

The probiotic potential of lactobacilli isolated from Nile tilapia (Oreochromis niloticus)’s intestine

The potential probiotic acid lactic bacteria isolated from Nile tilapia (Oreochromis niloticus)fs intestine was tested for fish farming. In our collection, 10 Lactobacillus strains were targeted to confront a series of antibiotics in order to draw their resistance profile, and to test their degree of inhibitory to four pathogenic bacteria, Staphylococcus aureus, Escherichia coli, Streptococcus sp. and Pseudomonas sp. The power of acidification and tolerance was tested. Overall, our results show that strains BLT31 and BLT21 are fully susceptible and resistance to the tested antibiotics. Strains BLT3, BLT20, BLT21 and BLT23 have a good antagonistic effect against pathogenic bacteria that cause the highest damage in aquaculture. For acid lactic production, strains BLT3, BLT26, BLT27, BLT28, and BLT31 are considered fast since Ģ pH . 4U in less than three hours. As for the resistance to pH and bile salts, two strains BLT3 and BLT31 showed significant power which gives them acceptable probiotic potential.Key words: Probiotics, antibiotics, aquaculture, the Nile Tilapia, inhibitory activities, lactobacilli

Design and Fabrication of Bioinspired Hierarchical Dissipative Elastic Metamaterials

Hierarchical structures with constituents over multiple length scales are found in various natural materials like bones, shells, spider silk and others, all of which display enhanced quasi-static mechanical properties, such as high specific strength, stiffness and toughness. At the same time, the role of hierarchy on the dynamic behaviour of metamaterials remains largely unexplored. This study assesses the effect of bio-inspired hierarchical organization as well as of viscoelasticity on the wave attenuation properties of continuous mechanical metamaterials. We consider single-phase metamaterials formed by self-similar unit cells with different hierarchical levels and types of hierarchy. Results highlight a number of advantages through the introduction of structural hierarchy. Band gaps relative to the corresponding non-hierarchical structures are mostly preserved, while additional "hierarchically-induced" band gaps appear. Additionally, the hierarchical configuration allows the tuning of the band gap frequencies of regular metamaterial to lower frequencies, with a simultaneous significant reduction of the global structural weight. We show that even small viscoelastic effects, not treated in the current literature, are essential in determining this behaviour. The approach we propose allows the addition of hierarchical elements to existing metamaterial configurations, with the corresponding improvement of the wave damping properties, thus providing indications for the design of structures for practical applications

Hierarchical large-scale elastic metamaterials for passive seismic wave mitigation

Large scale elastic metamaterials have recently attracted increasing interest in the scientific community for their potential as passive isolation structures for seismic waves. In particular, so-called "seismic shields"have been proposed for the protection of large areas where other isolation strategies (e.g. dampers) are not workable solutions. In this work, we investigate the feasibility of an innovative design based on hierarchical design of the unit cell, i.e. a structure with a self-similar geometry repeated at different scales. Results show how the introduction of hierarchy allows the conception of unit cells exhibiting reduced size with respect to the wavelength while maintaining the same or improved isolation efficiency at frequencies of interest for earthquake engineering. This allows to move closer to the practical realization of such seismic shields, where low-frequency operation and acceptable size are both essential characteristics for feasibility

Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani

Citation: Chandrasekaran, M., Chandrasekar, R., Sa, T., & Sathiyabama, M. (2014). Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani. Retrieved from http://krex.ksu.eduSerine proteases generally share a relatively high degree of sequence identity and play a major role in the diversity of biological processes. Here we focus on three-dimensional molecular architecture of serine proteases from Alernaria solani. The difference in flexibility of active binding pockets and electrostatic surface potential distribution of serine proteases in comparison with other fungal species is reported in this study. In this study we have purified a serine protease from the early blight pathogen, Alernaria solani. MALDI-TOF-MS/MS analysis revealed that protease produced by A. solani belongs to alkaline serine proteases. AsP is made up of 403 amino acid residues with molecular weight of 42.1kDa (Isoelectric point (pI)-6.51) and molecular formula C[subscript 1859]H[subscript 2930]N[subscript 516]O[subscript 595]S[subscript 4]. The follow-up research on the molecular structure prediction is used for assessing the quality of A. solani Protease (AsP). The AsP protein structure model was built based on its comparative homology with serine protease using the program, MODELER. AsP had 16 β-sheets and 10 α-helices, with Ser[superscript 350] (G347-G357), Asp[superscript 158] (D158-H169) and His[superscript 193] (H193-G203) in separate turn/coil structures. Biological metal binding region situated near the 6th-helix and His[superscript 193] residue is responsible for metal binding site. In addition, the calcium ion is coordinated by the carboxyl groups of Lys[superscript 84], Ile[superscript 85], Lys[superscript 86], Asp[superscript 87], Phe[superscript 88], Ala[superscript 89], Ala[superscript 90] (K84-A90) for first calcium (Ca[superscript 2+]) binding site and carbonyl oxygen atom of Lys[superscript 244], Gly[superscript 245], Arg[superscript 246], Thr[superscript 247], Lys[superscript 248], Lys[superscript 249], and Ala[superscript 250] (K244–A250), for second Ca[superscript 2+] binding site. Moreover, Ramachandran plot analysis of protein residues falling into most favored secondary structures were determined (83.3%). The predicted molecular 3D structural model was further verified using PROCHECK, ERRAT and VADAR servers to confirm the geometry and stereo-chemical parameters of the molecular structural design. The functional analysis of AsP 3D molecular structure predictions familiar in the current study may provide a new perspective in the understanding and identification of antifungal protease inhibitor designing

Application of a Laser-Based Time Reversal Algorithm for Impact Localization in a Stiffened Aluminum Plate

Non-destructive testing and structural health monitoring (SHM) techniques using elastic guided waves are often limited by material inhomogeneity or geometrical irregularities of the tested parts. This is a severe restriction in many fields of engineering such as aerospace or aeronautics, where typically one needs to monitor composite structures with varying mechanical properties and complex geometries. This is particularly true in the case of multiscale composite materials, where anisotropy and material gradients may be present. Here, we provide an impact localization algorithm based on time reversal and laser vibrometry to cope with this type of complexity. The proposed approach is shown to be insensitive to local elastic wave velocity or geometrical features. The technique is based on the correlation of the measured impact response and a set of measured test data acquired at various grid points along the specimen surface, allowing high resolution in the determination of the impact point. We present both numerical finite element simulations and experimental measurements to support the proposed procedure, showing successful implementation on an eccentrically stiffened aluminum plate. The technique holds promise for advanced SHM, potentially in real time, of geometrically complex composite structures

Mathematical modelling and evaluation in synthetic structured credit

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