The Effects of Salt Stress on Certain Physiological Parameters in Summer Savory (Satureja hortensis L.) Plants

Savory plants were treated with different concentrations of NaCl. Plants were grown under controlled environment and harvested after 42 days for measurements of biochemical and physiological parameters. The essential oil of dryed aerial parts of treated plants were isolated and analyzed with GC/MS. The main essential oil compounds were determined as carvacrol (55.37%) and g-terpinene (32.92%) in control plants. In NaCl treated plants, with increasing NaCl, carvacrol content increased and g-terpinene decreased. In all the plants treated with NaCl, growth parameters, pigments contents and photosynthetic rate were decreased, while, proline and soluble sugars contents increased.Our results indicated that with increasing salinity, carvacrol amount increased which can be considered for medical usages

Cavitation in Thermoplastic Melts: New Insights into Ultrasound-Assisted Fibre-Impregnation

The impregnation of continuous carbon fibre roving with thermoplastic melt is a challenging task due to the high viscosity and surface tension of the melt. A new technique is under development utilizing ultrasonic oscillations within the thermoplastic melt which encloses a fibre roving, to achieve fibre impregnation. Despite ultrasonic processing being very efficient, the specific conditions created in the thermoplastic melt have never been studied before. This study investigated whether cavitation effects could be present during ultrasound-assisted fibre impregnation. The observed acoustic effects allowed us to suggest the possible underlying mechanisms. For the purpose of the study a melt-bath impregnation setup with polylactide was built. To detect the cavitation effects and acoustic parameters a calibrated high-temperature cavitometer was used. The results showed the formation of small cavitation zones in the direct vicinity to the sonotrode tip where the fibre roving would be positioned. Therefore, the occurrence of cavitation was established, and induced effects like shock waves, microjets and microstreaming should be further considered for detailed investigation of the ultrasound-assisted impregnation mechanism.This research study was supported for IT, MK and DE by the UK Engineering and Physical Sciences Research Council (EPSRC) through the UltraMelt2 (grant numbers EP/R011001/1, EP/R011095/1 and EP/R011044/1) project

Transcript-indexed ATAC-seq for precision immune profiling.

T cells create vast amounts of diversity in the genes that encode their T cell receptors (TCRs), which enables individual clones to recognize specific peptide-major histocompatibility complex (MHC) ligands. Here we combined sequencing of the TCR-encoding genes with assay for transposase-accessible chromatin with sequencing (ATAC-seq) analysis at the single-cell level to provide information on the TCR specificity and epigenomic state of individual T cells. By using this approach, termed transcript-indexed ATAC-seq (T-ATAC-seq), we identified epigenomic signatures in immortalized leukemic T cells, primary human T cells from healthy volunteers and primary leukemic T cells from patient samples. In peripheral blood CD4+ T cells from healthy individuals, we identified cis and trans regulators of naive and memory T cell states and found substantial heterogeneity in surface-marker-defined T cell populations. In patients with a leukemic form of cutaneous T cell lymphoma, T-ATAC-seq enabled identification of leukemic and nonleukemic regulatory pathways in T cells from the same individual by allowing separation of the signals that arose from the malignant clone from the background T cell noise. Thus, T-ATAC-seq is a new tool that enables analysis of epigenomic landscapes in clonal T cells and should be valuable for studies of T cell malignancy, immunity and immunotherapy

Characterization of shock waves in power ultrasound

© The Author(s), 2021. The application of cavitation-induced shock waves generated at low driving frequencies, known as power ultrasound, is essential for a wide range of fields, such as sonochemistry, lithotripsy, nanomaterials, emulsions and casting, to name but a few. In this paper, we present measurements of the shock wave pressures emitted by cavitating bubbles in water, under ultrasonic excitation produced by an immersed probe oscillating at 24 kHz. A broad-spectrum fibre-optic hydrophone calibrated in the range of 1–30 MHz was used for this purpose. Spectral analysis of the data reveals a consistent resonance peak at a very narrow range of frequencies (3.27–3.43 MHz). Results were confirmed using real-time analysis of high-speed recordings. By eliminating other possible sources, we propose that this new peak might be associated with shock wave emissions from collapsing bubbles. Spatial maps obtained by collating individual shock wave pressures highlight the effect of pressure shielding with increasing input power, attributed to a cloud of bubbles surrounding the probe. This work contributes towards the elucidation of the key properties of cavitation-driven shock waves and the underlying mechanisms, essential in controlling the effectiveness of the external processing conditions on various physical, chemical and biological systems.UK Engineering and Physical Sciences Research Council (EPSRC) through the UltraMelt2 (grants EP/R011001/1, EP/R011095/1 and EP/R011044/1) and EcoUltra2D (grants EP/R031401/1, EP/R031665/1, EP/R031819/1 and EP/R031975/1) projects

Mechanical design and development of TES bolometer detector arrays for the Advanced ACTPol experiment

The next generation Advanced ACTPol (AdvACT) experiment is currently underway and will consist of four Transition Edge Sensor (TES) bolometer arrays, with three operating together, totaling ~5800 detectors on the sky. Building on experience gained with the ACTPol detector arrays, AdvACT will utilize various new technologies, including 150mm detector wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors includes a feedhorn array of stacked silicon wafers which form a spline profile leading to each pixel. This is then followed by a waveguide interface plate, detector wafer, back short cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured from high purity copper and then gold plated. In addition to the detector array assembly, the array package also encloses cryogenic readout electronics. We present the full mechanical design of the AdvACT high frequency (HF) detector array package along with a detailed look at the detector array stack assemblies. This experiment will also make use of extensive hardware and software previously developed for ACT, which will be modified to incorporate the new AdvACT instruments. Therefore, we discuss the integration of all AdvACT arrays with pre-existing ACTPol infrastructure.Comment: 9 pages, 5 figures, SPIE Astronomical Telescopes and Instrumentation conference proceeding

Techno-Economic analysis of stand-alone hybrid energy system for the electrification of iran drilling oil rigs

This paper explores the potential of use of stand-alone hybrid wind/solar energy system in electrification of calibrating equipment of drilling oil rig in Iran. To achieve this, different hybrid energy system configurations based on calibration equipment demand are proposed. This study puts emphasis on the energy production and cost of energy from both wind turbine and photovoltaic (PV) in the hybrid system. In addition, to make conditions more realistic, the real meteorological data is used for HOMER software to perform the technical and economic analysis of the hybrid system. Results indicate that the PV array shares more electricity production than the wind turbine generator if both wind turbine and PV array are utilized in the wind/solar hybrid system. Moreover, results show that the operational cost will be reduced by the suggested hybrid system

Scale Up Design Study on Process Vessel Dimensions for Ultrasonic Processing of Water and Liquid Aluminium

UK Engineering and Physical Sciences Research Council (EPSRC) through the UltraMelt2 (grant EP/R011001/1, EP/R011095/1 and EP/R011044/1) and EcoUltra2D (grant EP/R031401/1, EP/R031665/1, EP/R031819/1, EP/ R031975/1) projects

Cyber-physical energy systems modeling, test specification, and co-simulation based testing

The gradual deployment of intelligent and coordinated devices in the electrical power system needs careful investigation of the interactions between the various domains involved. Especially due to the coupling between ICT and power systems a holistic approach for testing and validating is required. Taking existing (quasi-) standardised smart grid system and test specification methods as a starting point, we are developing a holistic testing and validation approach that allows a very flexible way of assessing the system level aspects by various types of experiments (including virtual, real, and mixed lab settings). This paper describes the formal holistic test case specification method and applies it to a particular co-simulation experimental setup. The various building blocks of such a simulation (i.e., FMI, mosaik, domain-specific simulation federates) are covered in more detail. The presented method addresses most modeling and specification challenges in cyber-physical energy systems and is extensible for future additions such as uncertainty quantification