Determining sucrose recovery in Saccarrum officinarum L. using regression and correlation analyses

Fourteen genotypes of sugarcane, Saccharium officinarum (L.), were evaluated for twelve characters and analyzed for genotypic correlation, phenotypic correlation and coefficients of determination were calculated through stepwise regression analysis to construct the regression models for selection of better sugarcane types. High variation was observed for all the characters including sucrose contents. Varying degrees of correlation coefficients were observed, genotypic being of higher magnitude thatindicated the genotypic influence for determining a particular trait. Stepwise regression analysis showed the effects of individual characters and the effects in combination contribute the maximum variation (36.0%) to sucrose percentage while two variable model using internodal length and number of tillers plant-1 that accounts for maximum variation of 40.1%. Variation for sucrose content on single factor basis was caused by tillers, therefore, this character was the most important to increase overall sucrose of S. officinarum L. However, second variable model exhibited that tillers and internodal length were important traits for increasing overall sucrose in sugarcane

Reproducible and expedient rice regeneration system using in vitro grown plants

Inevitable prerequisite for expedient regeneration in rice is the selection of totipotent explant and developing an apposite combination of growth hormones. Here, we reported a reproducible regeneration protocol in which basal segments of the stem of the in vitro grown rice plants were used as ex-plant. Using the protocol, callus was developed from the exposed cells of root segments and cortical tissues of basal part of the stem. Various levels of 2,4-dichlorophenoxyacetic acid (2,4-D) were used, where 1 mg/L was found as best level for callus induction. Further, 25 combinations of kinetin and naphthalene acetic acid (NAA) were developed to investigate the regeneration response of the calli. The root-derived calli did not respond to any combination at all, whereas the nodular calli derived from the stem segments responded variably to kinetin and NAA combinations in the Murashige and Skoog (MS) medium from base to top. Higher kinetin to NAA ratios promoted embryogenesis, whereas lower ratios exhibited rhizogenesis. A combination of 3 mg/L kinetin and 1 mg/L NAA was established to be the best combination for plant regeneration through embryogenesis in rice from in vitro grown plants. Plants regenerated in vitro were successfully acclimatized in the pots, where they exhibited phenotypically indistinguishable normal growth when compared with plants developed from seed, hence the developed regeneration system for rice in these studies may be treated as one of the best strategies to in vitro clonal propagation and purification, ahead of seasonal growth of plants in the field or green house.Key words: Rice, in vitro regeneration, explants, naphthalene acetic acid (NAA), kinetin, rhizogenesis

Estimation of inorganic constituents in the seeds of blue and white flowering capitulum of Silybum marianum

Silybum Marianum commonly known as milk thistle contains flavonolignans, collectively known as silymarin. The main components of silymarin are silybine, isosilybine, silychristin and silydinine. This study was aimed to estimate inorganic constituents in blue and white capitulum’s seeds from different areas of KPK. Concentration of Na was found to be high (6 mg/kg) in white capitulum seeds from Karak area, while K concentration was high (6 mg/kg) in the blue capitulum seeds from Khyber agency. High concentration of Ca (20 mg/kg) was seen in both white and blue capitulum’s seeds of Khyber agency. Less concentration of NO3 (0.09 mg/kg) was detected in white capitulum seeds from Kohat district and high concentration of SO4 (22.14 mg/kg) was recorded in blue capitulum seeds collected from Peshawar.Key words: Silybum marianum, capitulum’s seeds, inorganic profile

Is integrated private-clinic based early child development care effective? A clustered randomised trial in Pakistan

Background In Pakistan, high prevalence of delays in early child development (ECD) is associated with poverty and lack of mothers’ caregiving skills. GP clinics, the main sources of care in poor urban localities, lack quality ECD care delivery. A contextualised intervention was developed and tested to enable GPs to deliver clinic-based, tool-assisted ECD counselling of mothers on a quarterly basis. Aim To assess the effectiveness of delivering a contextualised ECD mother-counselling intervention. Design & setting Clustered randomised controlled trial, in poor urban localities of Pakistan. Locality clusters were allocated to intervention and control arm using simple randomisation. Method A total of 2327 mother–child pairs were recruited at 32 GP clinics, one from each cluster-locality; 16 GP clinics per arm. The clinic-based counselling intervention covering child stimulation, nutrition, and maternal mental health was delivered mainly by clinic assistants to mothers at ≤6 weeks, and 3, 6, and 9 months of child age. At 12 months of child age, each mother–child pair was assessed for the primary outcome, that is, delays in the five development domains (determined by Ages and Stages Questionnaire-3 [ASQ-3] score); and secondary outcomes, namely the prevalence of stunting and maternal depression (determined by Patient Health Questionnaire-9 [PHQ-9] score). The outcome assessors were blinded to the cluster–arm allocation. Outcome analyses were calculated on cluster-level. Results At 12 months, the number of children with delay in two or more development domains was significantly lower in the intervention arm (-0.17 [95% confidence interval {CI} = -0.26 to -0.09]; P<0.001) compared to the control arm. The difference in the prevalence of child stunting and maternal depression were also significant at -0.21% (95% CI = -0.30 to -0.13; P<0.001) and -0.23% (95% CI = -0.29 to -0.18; P = 0.000) respectively. Conclusion Contextualised ECD care, when delivered at GP clinics in poor urban localities, can effectively reduce the developmental delays during the first 12 months of the child's life

A realistic path loss model for real-time communication in the urban grid environment for Vehicular Ad hoc Networks

Wireless signal transmission is influenced by environmental effects. These effects have also been challenging for Vehicular Ad hoc Network (VANET) in real-time communication. More specifically, in an urban environment, with high mobility among vehicles, a vehicle’s status from the transmitter can instantly trigger from line of sight to non-line of sight, which may cause loss of real-time communication. In order to overcome this, a deterministic signal propagation model is required, which has less complexity and more feasibility of implementation. Hence, we propose a realistic path loss model which adopts ray tracing technique for VANET in a grid urban environment with less computational complexity. To evaluate the model, it is applied to a vehicular simulation scenario. The results obtained are compared with different path loss models in the same scenario based on path loss value and application layer performance analysis. The proposed path loss model provides higher loss value in dB compared to other models. Nevertheless, the performance of vehicle-vehicle communication, which is evaluated by the packet delivery ratio with different vehicle transmitter density verifies improvement in real-time vehicle-vehicle communication. In conclusion, we present a realistic path loss model that improves vehicle-vehicle wireless real-time communication in the grid urban environment

Ethnobotanical, phytochemical and pharmacological aspects of daphne mucronata (thymeleaceae)

Daphne mucronata is a shrub well known as a medicinal plant in different regions of Asia. Ethnobotanical, phytochemical and pharmacological studies have revealed strong anti-cancer potential of the plant. Literature reports the evaluation of the initial bioactivity profile and extraction of the plant followed by different chromatographic techniques to obtain fractions. As an outcome,  isolation and identification of coumarins, flavonoids, triterpenoids, lignin  cumarinolignans, glucosides, daphnecin, aquillochin, daphnine and umbelliferone from the plant have been reported. Of these compounds, a diterpene, named gnidilatimonoein, has shown promising anticancer potency in in vitro tests on various cancer cell lines. This review article is an effort to summarize literature published in recent years on the bioactivity of Daphne mucronata

Analysis of chemical constituents in medicinal plants of selected districts of Pakhtoonkhwa, Pakistan

Alkaloids, flavonoids and saponins were determined in the Teraxacam officinale, Cichorium intybus and Figonia tritica. Quantative determinations of crude alkaloids, flavonoids and saponins (g kg-1) and their percentages (%) were determined in C. intybus, T. officinale and F. critica collected from Kohat, Mardan, Nowshera and Peshawar regions. Thin layer chromatography (TLC) study of the alkaloids, flavonoids and saponins were carried out with different solvent systems and color of the spot and Rf value of each constituent was determined. Among the samples of C. intybus, relatively higher contents of alkaloids (14 g kg-1) were found in the sample collected from Mardan region, followed by the sample gathered from Nowshera which is equal to 13.8g kg-1. Higher contents of flavonoids were found in C. intybus collected from Kohat region which was 23.48 g kg-1. The other remaining samples of C. intybus ranged in the decreasing order of 19.7, 17.2 and 10.0 g kg-1 for the ones collected from Nowshera, Mardan and Peshawar, respectively. The overall saponin contents remained almost low as compared to the alkaloid and flavonoid contents.Key words: Alkaloids, flavonoids, saponins, TLC study, medicinal plants

Heavy metals and inorganic constituents in medicinal plants of selected Districts of Khyber Pakhtoonkhwa, Pakistan

Heavy metals such as Cr, Fe, Zn, Mn, Ni, Pb, Cu and Cd, and inorganic ions like HCO3-, CO32-, Ca2+, Mg2+, Cl-, Na+, SO42-, NO3-, Fe2+ and F- were investigated in medicinally important plants: Taraxacam officinale, Cichorium intybus and Figonia critica, applying atomic absorption spectrophotometer techniques. In the studied herbs, there were variable amounts of heavy metals and inorganic ions. The purpose of this study was to standardize heavy metals contamination in various indigenous medicinal plants and to create awareness among the public regarding its safe use at collection areas (containing high level of heavy metals) and their adverse health effects.Key words: Medicinal plants, inorganic constituents, heavy metals, atomic absorption spectrophotometer

Complete larval development of the hermit crabs Clibanarius aequabilis and Clibanarius erythropus (Decapoda : Anomura : Diogenidae), under laboratory conditions, with a revision of the larval features of genus Clibanarius

The complete larval development (four zoeae and one megalopa) of Clibanarius aequabilis and C. erythropus, reared under laboratory conditions, is described and illustrated. The larval stages of the two northeastern Atlantic Clibanarius species cannot be easily differentiated. Their morphological characters are compared with those of other known Clibanarius larvae. The genus Clibanarius is very homogeneous with respect to larval characters. All Clibanarius zoeae display a broad and blunt rostrum, smooth abdominal segments and an antennal scale without a terminal spine. Beyond the second zoeal stage, the fourth telson process is present as a fused spine, and the uropods are biramous. In the fourth larval stage all species display a mandibular palp. The Clibanarius megalopa presents weakly developed or no ocular scales, symmetrical chelipeds, apically curved corneous dactylus in the second and third pereiopods, and 5-11 setae on the posterior margin of the telson. Apart from the number of zoeal stages, Clibanarius species may be separated, beyond the second zoeal stage, by the telson formula and the morphology of the fourth telson process.info:eu-repo/semantics/publishedVersio