Calcifying epithelial odontogenic tumor in a child: A case report

Calcifying epithelial odontogenic tumor (CEOT) or Pindborg tumor is a rare tumor that accounts for <1% of all odontogenic tumors. It usually affects patients between the 3rd and 4th decades of life, however a wide age range from 8 to 92 years has been reported. This neoplasm may be associated with erupted or unerupted teeth. There are both intraosseous and extraosseous variants of CEOT and the posterior part of mandible is the most common location. We present an interesting case of CEOT involving the left side of the maxilla associated with unerupted canine and premolar in an 11 year old girl. Keywords: Odontogenic tumor; Pindborg; Pathology

Comparative Evaluation of the efficacy of Laser Therapy and Fibroblastic Growth Factor Injection on Mucosal Wound Healing in Rat Experimental Model

Introduction: The aim of present study was to compare the effects of laser and bFGF treatment on operative wound healing in a rat model.Methods: 66 male Wistar rats were employed in this study. 10millimeter surgical wound was created on buccal mucosa of each rat ,under anesthesia, and then the rats were divided into 3 groups of 22 each: 1- GF group (received subcutaneous injection of bFGF), 2- laser group (treated with low level laser irradiation) and 3- control group (received no treatment). On day 5 half of rats in each group and on day 10 the other half, were sacrificed. Then, samples were taken from rats' buccal mucosa for histological assay and scoring. The data was analyzed with Mann-Whitney test (α=5%).Results: On day5 there was not any significant difference between GF and control group; however laser group showed delayed wound coverage clinically, compared to other groups(p&lt;0.05). On day 10, histological examination demonstrated marked vascular granulation tissue in GF group. Collagen production was significantly prominent in laser group compared to GF treated samples (p=.004). Inflammation of granulation tissue in GF and laser groups was significantly less than that in control samples (p=.005 and .001, respectively). Conclusion: The components of wound matrix induced by GF and laser treatment were significantly different. Although bFGF or laser treatment of oral wounds, under conditions of present study, did not accelerated wound healing but showed some other notable effects on the quality of healing

The efficacy of photodynamic therapy in rat tongue dysplasia

Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) has previously shown promising results in cancerous cell destruction. The present study was conducted to evaluate the efficacy of this treatment option on oral epithelial dysplasia in Wistar rats. Furthermore, microscopic effects of systemic versus topical administration of ALA before laser illumination was assessed. Thirty male Wistar rats (200- 250 grams) were used in the present study. Tongue dysplasia was induced by a daily delivery of a 20 ppm solution of 4-nitroquinoline -1- oxide (4NQO) for 3 months. Then, rats were divided into 3 groups of 10 including, group 1 that was received systemic ALA-based PDT (30 mg/kg ALA), group 2 that was received topical ALA-based PDT (20% ALA solution) and group 3 (control) which was left untreated. Tongue specimens were fixed for histopathological evaluation and dysplasia was graded at microscopic level. Data was compared between various treatment groups using Mann Whitney test (p<0.05). The rate of atypical dysplastic cells was decreased significantly in both topical (p= 0.006) and systemic (p= 0.001) treatment groups compared to control group. Furthermore, systemic use of ALA resulted in a remarkable destruction of dysplastic cells compared to its topical application (p=0.045). Nevertheless, some evidence of muscle destruction was documented in systemic ALA group. It seems that ALA mediated PDT is an effective treatment option for the destruction of dysplastic cells. However, the extent of this effect depends on the mode of ALA administration before light illumination

Clinicopathological study of 229 cases of salivary gland tumors in Isfahan population

Background: Salivary gland tumors are relatively uncommon and they consist of 3-10% of head and neck neoplasms. Most of studies have shown geographic variation in the incidence and histopathologic types of salivary gland neoplasms. The aim of this study was to evaluate the salivary gland tumors in Isfahan for 10 years duration. Materials and Methods: In this epidemiological study, 229 medical records of patients with salivary gland neoplasms in Isfahan for 10 years duration (January 2001-December 2011) were reviewed. The clinical data and histopathological features were statistically analyzed using the Chi-square, analysis of variance and Fisher tests; P < 0.05 was considered to be significant. Results: The data showed that salivary gland tumors were most frequent in women. The mean age of patients with benign tumors was 41.26 years and 51.83 years in malignant tumors. Out of 229 salivary gland neoplasms, 127 (55.5%) were benign and 102 (44.5%) were malignant. Most were in parotid (105), followed by the minor salivary glands (95), the submandibular gland (28) and the sublingual gland (1). Conclusion: Pleomorphic adenoma is the most common salivary gland tumor and mucoepidermoid carcinoma followed by adenoid cystic carcinoma are the most common malignancy of salivary gland tumors in Isfahan population. Although most of the results of this study were similar to those reported in other populations, some differences were observed

A case of central mucoepidermoid carcinoma associated with dentigerous cyst

Mucoepidermoid carcinoma (MEC) is the most common malignant salivary gland neoplasm. Central MEC (CMEC) is a rare primary intraosseous bony lesion with an incidence of 2%–4.3% of all MECs reported. In this article, we present a rare case of a CMEC in the anterior region of maxilla at a 43-year-old female patient that was arising from a dentigerous cyst. CMECs are extremely rare tumor. They are usually low-grade lesions with favorable prognosis. Odontogenic cysts are one of the origins of this lesion. Treatment of impacted tooth is necessary in the early stage for prevention of this neoplasm

Cyclin D1 and Ki-67 expression and its correlation with histopathological parameters and cervical lymph node metastasis in oral squamous cell carcinoma

Background: Oral squamous cell carcinoma (OSCC) is the most common malignant tumor among oral cancers. Cyclin D1 and Ki-67 have associated with cell division. The aim of this study was to compare the expression of these markers in OSCC with and without cervical lymph node (LN) metastasis. Materials and Methods: This cross-sectional study was performed on 40 OSCCs with and without cervical LN metastasis (20 in each group) that was recorded in the pathology archive of Ayatollah Kashani Hospital in Isfahan. Clinical information including age, gender, and location was collected. Some histopathological parameters including depth of invasion, lymphovascular invasion (LVI), perineural invasion (PNI), number of LN metastases, histopathological grade, and stage of disease were evaluated. Immunohistochemical staining was performed for cyclin D1 and Ki-67. All data were entered into SPSS24 software and were analyzed by Mann–Whitney, Kruskal–Wallis, Chi-square, Fisher's exact, and t-tests. P < 0.05 was considered statistically significant. Results: Based on LVI and stage of disease, a significant correlation was found between the two groups (P < 0.001). There was a significant difference between the two groups based on cyclin D1 expression (P = 0.05). The expression of the Ki-67 showed a significant difference based on tumor location (P = 0.026) and PNI (P = 0.033). Conclusion: The use of markers should be considered in determining the prognosis of OSCC, and the cyclin D1 marker is one of the useful markers for predictors of cervical LN metastasis

Physiological and electrophysiological evaluation of the hearing system in low birth weight neonates treated with cholestin: a cohort study

Abstract Background Infections in neonates are mainly caused by sepsis and are the most important complications of low birth weight. In the treatment of these infections, it is common to use broad-spectrum antibiotics such as cholestin. Cholestin can cause changes in the latency of brainstem-evoked response waves. The current cohort study tried to investigate the probability of changes caused by cholestin in LBW neonates and benefiting from two common tests to identify the hearing system in neonates after treatment by cholestin and compared them with a control group. Methods The study was an exposure-based cohort, during which hearing damage caused by receiving cholestin was evaluated in 104 low birth weight neonates, in two groups (52 exposure and 52 no-exposure to cholestin). OAE and ABR tests were performed at the age of 3 months to identify complications in two groups and their results were compared. Results The absolute latency of waves I, III, and V in the brainstem evoked response test with an intensity of 80 dB Hl showed a significant difference in the exposure group with the control group. The interpeak latency of the waves as well as the effect of the drug on the gender of the infants did not show significant differences with the control group. Conclusion Cholestin did not affect the axonal or synaptic transmission time of the auditory nerve to the brainstem. It is possible that the simultaneous presence of risk factors, such as the use of cholestin, low birth weight, noises in the intensive care unit, and other unknown factors, can be effective in the change of the absolute latency of waves