Genetic Polymorphism of Prolactin, Bone Morphogenetic Protein Receptor 1B and Insulin-like Growth Factor 1 Genes in Two Selected Lines of Japanese Quail

Abstract: Quail is the smallest avian species raised for meat and egg production. It has several advantages as a laboratory bird for biological and biomedical investigations. In this study, we performed single nucleotide polymorphism (SNP) . DNA was extracted from blood samples using commercial kits and amplified using polymerase chain reaction (PCR). Nucleotide polymorphisms between two selected lines were detected by DNA sequencing. Five nucleotide changes in PRL and BMPR-1B genes were identified and there was no nucleotide difference in IGF-1 gene between egg and meat selected lines. The further study was required to find mutation in other site of IGF-1 and the SNPs discovered in this study provided suitable markers for association studies of candidate genes with important economic traits in Japanese quail

Association of polymorphisms in kappa casein gene with milk traits in Holstein Friesian cattle

The genetic polymorphism of milk proteins can be a useful guide for selection and an informative marker in breeding research. Kappa casein (CSN3) is a standout amongst the most vital milk proteins in the mammals that assumes a crucial part in the milk quality and coagulation. Considering polymorphism of CSN3 and its relationship with milk characteristics in Holstein Friesian cattle was the target of the current study. The PCRRFLP utilizing Hind III endonuclease enzyme and DNA sequencing were performed on DNA samples extracted from fifty animals. Restriction digestion analysis of 633bp PCR product indicated two genotypes AA (uncut 633 bp), and AB (633, 416, and 217 bp) with higher frequency of A allele (0.80) than B allele (0.20). Animals with AB genotypes had a significantly higher milk yield and SNF % (10724 and 9.26%, respectively), whereas animals with AA genotype had a superior estimate effect on fat (3.36%) and proteins (3.14%). Comparison of the nucleotide sequences between different genotypes revealed only one nucleotide changes (A405G), corresponding to the same amino acid residue alanine. Molecular selection for animals carrying the B allele could impact breeding programs for dairy production in Egypt

Detection of SNPs in growth hormone and insulin like growth factor -1 genes in two divergently selected lines of Japanese quail

The Japanese quail (Coturnix japonica) is used for producing both meat and egg in many countries and as a model for animal research purposes. Two lines of Japanese quail that were differentiated by high body weight (HBW) and low body weight (LBW) had been improved by selection for body weight at four weeks of age. The objective of this study was to detect single nucleotide polymorphism (SNP) in Growth hormone (GH) and Insulin like Growth Factor -1 (IGF-1) genes in the two Japanese quails selected lines which based on the weight of the body at four weeks of age. DNA has been extracted from fifty blood samples by commercial kits and amplified by polymerase chain reaction (PCR). DNA sequencing revealed nucleotide polymorphisms between the two Japanese quails selected lines. The results of this investigation revealed that, one nucleotide change (T/C) in the intron 2 of GH gene. However, there were no nucleotide differences in IGF-1gene between the two selected lines. It concluded that, the SNP discovered in the GH gene may provide appropriate markers for associating researches of candidate genes with imperative economic measurements in Japanese quail. However, further studies are necessitating detecting mutation in another region of IGF-1