Reversing age: Dual species measurement of epigenetic age with a single clock

Young blood plasma is known to confer beneficial effects on various organs in mice. However, it was not known whether young plasma rejuvenates cells and tissues at the epigenetic level; whether it alters the epigenetic clock, which is a highly-accurate molecular biomarker of aging. To address this question, we developed and validated six different epigenetic clocks for rat tissues that are based on DNA methylation values derived from n=593 tissue samples. As indicated by their respective names, the rat pan-tissue clock can be applied to DNA methylation profiles from all rat tissues, while the rat brain-, liver-, and blood clocks apply to the corresponding tissue types. We also developed two epigenetic clocks that apply to both human and rat tissues by adding n=850 human tissue samples to the training data. We employed these six clocks to investigate the rejuvenation effects of a plasma fraction treatment in different rat tissues. The treatment more than halved the epigenetic ages of blood, heart, and liver tissue. A less pronounced, but statistically significant, rejuvenation effect could be observed in the hypothalamus. The treatment was accompanied by progressive improvement in the function of these organs as ascertained through numerous biochemical/physiological biomarkers and behavioral responses to assess cognitive functions. Cellular senescence, which is not associated with epigenetic aging, was also considerably reduced in vital organs. Overall, this study demonstrates that a plasma-derived treatment markedly reverses aging according to epigenetic clocks and benchmark biomarkers of aging.Fil: Horvath, Steve. University of California at Los Angeles; Estados UnidosFil: Singh, Kavita. NMIMS University; IndiaFil: Raj, Ken. Public Health England; Reino UnidoFil: Khairnar, Shraddha. NMIMS University; IndiaFil: Sanghav, Akshay. Nugenics Research Pvt Ltd; IndiaFil: Shrivastava, Agnivesh. Nugenics Research Pvt Ltd; IndiaFil: Zoller, Joseph A.. University of California at Los Angeles; Estados UnidosFil: Li, Caesar Z.. University of California at Los Angeles; Estados UnidosFil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Canatelli Mallat, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Lehmann, Marianne. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Solberg Woods, Leah C.. Wake Forest University School of Medicine; Estados UnidosFil: Garcia Martinez, Angel. University of Tennessee; Estados UnidosFil: Wang, Tengfei. University of Tennessee; Estados UnidosFil: Chiavellini, Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Levine, Andrew J.. University of California at Los Angeles; Estados UnidosFil: Chen, Hao. University of Tennessee; Estados UnidosFil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Katcher, Harold L.. Nugenics Research Pvt Ltd; Indi

Advance Traffic Signal Controlling System Using RFID Technique

An design for making intelligent systems for dominant road traffic is projected. The system relies on a straightforward principle of RFID pursuit of vehicles, will operate in period of time, improve traffic flow and safety, and totally machine-driven, saving pricey constant human involvement. these days the transportation systems show new challenges in management and management problems thanks to quantity of vehicles and therefore the variability and extension of the roads. New technologies are developed to induce data concerning Traffic in cities. however, new frameworks with completely different technologies applied to traffic observation haven't been well outlined. In this, frequency Identification technology (RFID) applied to road Traffic observation is exposed

Reversal of biological age in multiple rat organs by young porcine plasma fraction

Young blood plasma is known to confer beneficial effects on various organs in mice and rats. However, it was not known whether plasma from young adult pigs rejuvenates old rat tissues at the epigenetic level; whether it alters the epigenetic clock, which is a highly accurate molecular biomarker of aging. To address this question, we developed and validated six different epigenetic clocks for rat tissues that are based on DNA methylation values derived from n = 613 tissue samples. As indicated by their respective names, the rat pan-tissue clock can be applied to DNA methylation profiles from all rat tissues, while the rat brain, liver, and blood clocks apply to the corresponding tissue types. We also developed two epigenetic clocks that apply to both human and rat tissues by adding n = 1366 human tissue samples to the training data. We employed these six rat clocks to investigate the rejuvenation effects of a porcine plasma fraction treatment in different rat tissues. The treatment more than halved the epigenetic ages of blood, heart, and liver tissue. A less pronounced, but statistically significant, rejuvenation effect could be observed in the hypothalamus. The treatment was accompanied by progressive improvement in the function of these organs as ascertained through numerous biochemical/physiological biomarkers, behavioral responses encompassing cognitive functions. An immunoglobulin G (IgG) N-glycosylation pattern shift from pro- to anti-inflammatory also indicated reversal of glycan aging. Overall, this study demonstrates that a young porcine plasma-derived treatment markedly reverses aging in rats according to epigenetic clocks, IgG glycans, and other biomarkers of aging

7th International Conference on Recent Advances in Mathematical Sciences and its Applications-2024: Abstract Book

This book presents the abstracts of the selected contributions to the 7th International Conference on Recent Advances in Mathematical Sciences and its Applications (RAMSA 2024), held on 29 February- 02 March 2024, by the Department of Mathematics, Jaypee Institute of Information Technology, Noida, India. RAMSA 2024 aims to assemble esteemed mathematicians, scientists, engineers, researchers from industry, and scholars, facilitating a platform for the exchange of ideas and discussions on recent advancements across various areas of mathematics. RAMSA-2024 provides an opportunity to delve into research findings and breakthroughs in mathematics, sciences, and engineering. This conference serves as a forum to address practical challenges encountered in different application domains and explore potential solutions. Conference Title: 7th International Conference on Recent Advances in Mathematical Sciences and its ApplicationsConference Acronym: RAMSA-2024Conference Date: 29 Feb-02 March 2024Conference Venue: Hybrid Mode (JIIT Noida & Online)Conference Organizer: Department of Mathematics, Jaypee Institute of Information Technology, Noida, Indi

7th International Conference on Recent Advances in Mathematical Sciences and its Applications-2024: Abstract Book

This book presents the abstracts of the selected contributions to the 7th International Conference on Recent Advances in Mathematical Sciences and its Applications (RAMSA 2024), held on 29 February- 02 March 2024, by the Department of Mathematics, Jaypee Institute of Information Technology, Noida, India. RAMSA 2024 aims to assemble esteemed mathematicians, scientists, engineers, researchers from industry, and scholars, facilitating a platform for the exchange of ideas and discussions on recent advancements across various areas of mathematics. RAMSA-2024 provides an opportunity to delve into research findings and breakthroughs in mathematics, sciences, and engineering. This conference serves as a forum to address practical challenges encountered in different application domains and explore potential solutions. Conference Title: 7th International Conference on Recent Advances in Mathematical Sciences and its ApplicationsConference Acronym: RAMSA-2024Conference Date: 29 Feb-02 March 2024Conference Venue: Hybrid Mode (JIIT Noida & Online)Conference Organizer: Department of Mathematics, Jaypee Institute of Information Technology, Noida, Indi