10 research outputs found
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CFD Modeling in Screw Compressors with complex multi rotor configurations
Computational Fluid Dynamics (CFD) of screw compressors is challenging due to the positive displacement nature of the process, existence of very fine fluid leakage paths, coexistence of working fluid and lubricant or coolant, fluid injection and most importantly the lack of methodologies available to generate meshes required for the full three dimensional transient simulations. In this paper, currently available technology of grid remeshing has been used to demonstrate the CFD simulations of complex multi rotor screw compressors like the twin screw compressor with parallel axis and single screw compressor with cylindrical main rotor and two planar gate rotors with perpendicular axis. Presently, methodology for grid generation of constant pitch twin screw machines is available through SCORG© (Kovacevic et al., 2007) but it is currently not suitable for different topologies like that of a single screw or variable pitch rotors. It is very challenging to handle the mesh deformation that happens in the compression chambers during operations of such machines. The methodology tested for this paper uses a technique called key-frame re-meshing in order to supply pre-generated grids to the CFD solver as the solution progresses. In order to evaluate accuracy of such approach, an adiabatic compression-expansion process in a reciprocating piston cylinder arrangement is studied and compared with a diffusion equation based mesh smoothing. It has been demonstrated, that although it is possible to simulate the complex configuration of screw compressors by key-frame re-meshing technique, there are many limitations with respect to the time consumed in pre-processing, demand to computational resources, accuracy of results and general difficulty to include advanced modeling features like turbulence, multispecies or multiphase flows. Hence it was concluded that customized tools for generation of CFD grids for such complex screw machines still remain to be developed
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Grid deformation strategies for CFD analysis of screw compressors
Customized grid generation of twin screw machines for CFD analysis is widely used by the refrigeration and air-conditioning industry today, but is currently not suitable for topologies such as those of single screw, variable pitch or tri screw rotors. This paper investigates a technique called key-frame re-meshing that supplies pre-generated unstructured grids to the CFD solver at different time steps. To evaluate its accuracy, the results of an isentropic compression-expansion process in a reciprocating piston cylinder arrangement have been compared. Three strategies of grid deformation; diffusion equation mesh smoothing, user defined nodal displacement and key-frame remeshing have been assessed. There are many limitations to key-frame re-meshing. It requires time consuming pre-processing, has limited applicability to complex meshes and leads to inaccuracies in conservation of calculated variables. It was concluded that customized tools for generation of CFD grids are required for complex screw machines
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Analytical Grid Generation for accurate representation of clearances in CFD for Screw Machines
One of the major factors affecting the performance prediction of twin screw compressors by use of computational fluid dynamics (CFD) is the accuracy with which the leakage gaps are captured by the discretization methods. The accuracy of mapping leakage flows can be improved by increasing the number of grid points on the profile. However, this method faces limitations when it comes to the complex deforming domains of a twin screw compressor because the computational time increases tremendously. In order to address this problem, an analytical grid distribution procedure is formulated that can independently refine the region of high importance for leakage flows in the interlobe space. This paper describes the procedure of analytical grid generation with the refined mesh in the interlobe area and presents a test case to show the influence of the mesh refinement in that area on the performance prediction. It is shown that by using this method, the flow domains in the vicinity of the interlobe gap and the blowhole area are refined which improves accuracy of leakage flow predictions
Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae)
The lac insects (Homoptera: Tachardiidae), belonging to the genus Kerria, are commercially exploited for the production of lac. Kerria lacca is the most commonly used species in India. RAPD markers were used for assessing genetic variation in forty-eight lines of Kerria, especially among geographic races, infrasubspecific forms, cultivated lines, inbred lines, etc., of K. lacca. In the 48 lines studied, the 26 RAPD primers generated 173 loci, showing 97.7% polymorphism. By using neighbor-joining, the dendrogram generated from the similarity matrix resolved the lines into basically two clusters and outgroups. The major cluster, comprising 32 lines, included mainly cultivated lines of the rangeeni form, geographic races and inbred lines of K. lacca. The second cluster consisted of eight lines of K. lacca, seven of the kusmi form and one of the rangeeni from the southern state of Karnataka. The remaining eight lines formed a series of outgroups, this including a group of three yellow mutant lines of K. lacca and other species of the Kerria studied, among others. Color mutants always showed distinctive banding patterns compared to their wild-type counterparts from the same population. This study also adds support to the current status of kusmi and rangeeni, as infraspecific forms of K. lacca
PDK1 and HR46 Gene Homologs Tie Social Behavior to Ovary Signals
The genetic basis of division of labor in social insects is a central question in evolutionary and behavioral biology. The honey bee is a model for studying evolutionary behavioral genetics because of its well characterized age-correlated division of labor. After an initial period of within-nest tasks, 2–3 week-old worker bees begin foraging outside the nest. Individuals often specialize by biasing their foraging efforts toward collecting pollen or nectar. Efforts to explain the origins of foraging specialization suggest that division of labor between nectar and pollen foraging specialists is influenced by genes with effects on reproductive physiology. Quantitative trait loci (QTL) mapping of foraging behavior also reveals candidate genes for reproductive traits. Here, we address the linkage of reproductive anatomy to behavior, using backcross QTL analysis, behavioral and anatomical phenotyping, candidate gene expression studies, and backcross confirmation of gene-to-anatomical trait associations. Our data show for the first time that the activity of two positional candidate genes for behavior, PDK1 and HR46, have direct genetic relationships to ovary size, a central reproductive trait that correlates with the nectar and pollen foraging bias of workers. These findings implicate two genes that were not known previously to influence complex social behavior. Also, they outline how selection may have acted on gene networks that affect reproductive resource allocation and behavior to facilitate the evolution of social foraging in honey bees
Mating alters gene expression patterns in Drosophila melanogaster male heads
<p>Abstract</p> <p>Background</p> <p>Behavior is a complex process resulting from the integration of genetic and environmental information. <it>Drosophila melanogaster </it>rely on multiple sensory modalities for reproductive success, and mating causes physiological changes in both sexes that affect reproductive output or behavior. Some of these effects are likely mediated by changes in gene expression. Courtship and mating alter female transcript profiles, but it is not known how mating affects male gene expression.</p> <p>Results</p> <p>We used <it>Drosophila </it>genome arrays to identify changes in gene expression profiles that occur in mated male heads. Forty-seven genes differed between mated and control heads 2 hrs post mating. Many mating-responsive genes are highly expressed in non-neural head tissues, including an adipose tissue called the fat body. One fat body-enriched gene, <it>female-specific independent of transformer </it>(<it>fit</it>), is a downstream target of the somatic sex-determination hierarchy, a genetic pathway that regulates <it>Drosophila</it> reproductive behaviors as well as expression of some fat-expressed genes; three other mating-responsive loci are also downstream components of this pathway. Another mating-responsive gene expressed in fat, <it>Juvenile hormone esterase </it>(<it>Jhe</it>), is necessary for robust male courtship behavior and mating success.</p> <p>Conclusions</p> <p>Our study demonstrates that mating causes changes in male head gene expression profiles and supports an increasing body of work implicating adipose signaling in behavior modulation. Since several mating-induced genes are sex-determination hierarchy target genes, additional mating-responsive loci may be downstream components of this pathway as well.</p
The Participation of Calponin in the Cross Talk between 20-Hydroxyecdysone and Juvenile Hormone Signaling Pathways by Phosphorylation Variation
20-hydroxyecdysone (20E) and juvenile hormone (JH) signaling pathways interact to mediate insect development, but the mechanism of this interaction is poorly understood. Here, a calponin homologue domain (Chd) containing protein (HaCal) is reported to play a key role in the cross talk between 20E and JH signaling by varying its phosphorylation. Chd is known as an actin binding domain present in many proteins including some signaling proteins. Using an epidermal cell line (HaEpi), HaCal was found to be up-regulated by either 20E or the JH analog methoprene (JHA). 20E induced rapid phosphorylation of HaCal whereas no phosphorylation occurred with JHA. HaCal could be quickly translocated into the nuclei through 20E or JH signaling but interacted with USP1 only under the mediation of JHA. Knockdown of HaCal by RNAi blocked the 20E inducibility of USP1, PKC and HR3, and also blocked the JHA inducibility of USP1, PKC and JHi. After gene silencing of HaCal by ingestion of dsHaCal expressed by Escherichia coli, the larval development was arrested and the gene expression of USP1, PKC, HR3 and JHi were blocked. These composite data suggest that HaCal plays roles in hormonal signaling by quickly transferring into nucleus to function as a phosphorylated form in the 20E pathway and as a non-phosphorylated form interacting with USP1 in the JH pathway to facilitate 20E or JH signaling cascade, in short, by switching its phosphorylation status to regulate insect development