Sanitary status of 47 pig manures in Brittany: comparison of the effectiveness of manure treatments on the levels of indicator bacteria and two pathogenic bacteria

The hygienic performance of three manure treatment systems (simple storage, biological treatment or thermal treatment) was evaluated for effluents collected from 47 piggeries across Brittany, France. Microbial analyses were carried out on raw manure, on the sludge stored in a tank after biological treatment and on the liquid phase stored in a lagoon after sludge settling or after thermal treatment. The effect of the treatments on E. coli, enterococci, Salmonella and Listeria monocytogenes was evaluated. The concentrations of indicator bacteria were highly variable regardless of the farm or the manure management

Sanitary status of 47 pig manures in Brittany: comparison of the effectiveness of manure treatments on the levels of indicator bacteria and two pathogenic bacteria

The hygienic performance of three manure treatment systems (simple storage, biological treatment or thermal treatment) was evaluated for effluents collected from 47 piggeries across Brittany, France. Microbial analyses were carried out on raw manure, on the sludge stored in a tank after biological treatment and on the liquid phase stored in a lagoon after sludge settling or after thermal treatment. The effect of the treatments on E. coli, enterococci, Salmonella and Listeria monocytogenes was evaluated. The concentrations of indicator bacteria were highly variable regardless of the farm or the manure management.</p

Changes in Concentrations of Fluoroquinolones and of Ciprofloxacin-resistant in Chicken Feces and Manure Stored in a Heap

International audienceThis study evaluated the impact of storing chicken manure on the degradation of enrofloxacin (ENR) and ciprofloxacin (CIP), and on the survival of CIP-resistant Enterobacteriaceae. At 24 d of age, half of 8900 chickens received ENR for 5 d. After the animals departed, their manure was stored in two heaps for 63 d. Enterobacteriaceae were cultured on media containing 0 to 32 mg L−1 of CIP. A total of 320 isolates were fingerprinted using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) to evaluate community structure. Initial concentrations of ENR and CIP in the heap were 22 and 1.8 mg kg−1, respectively. Seventy-three percent of the two fluoroquinolones were eliminated during storage. The administration of ENR led to a 5.1 log10 decrease in Enterobacteriaceae concentrations and emergence of CIP-resistant bacteria, which became dominant in the feces. Enterobacteriaceae concentrations decreased 1.2 to 2.3 log10 2 d after the heaps were made and continued to decline during storage. No resistant Enterobacteriaceae were found by Day 63. The highest CIP minimum inhibitory concentration (MIC) values observed among isolates of Escherichia coli and of both Proteus mirabilis and Providencia sp. were 128 and 4 mg L−1, respectively. The dominant ERIC-PCR profiles changed over time. There was no relationship between genotype and resistance-isolated strains to CIP. Storing chicken manure in heaps appeared to be an effective way of limiting the entrance of CIP-resistant E. coli into the environment but did not prevent the dissemination of fluoroquinolones after land spreading.</p