12,329 research outputs found
Detection of amblyopia utilizing generated retinal reflexes
Investigation confirmed that GRR images can be consistently obtained and that these images contain information required to detect the optical inequality of one eye compared to the fellow eye. Digital analyses, electro-optical analyses, and trained observers were used to evaluate the GRR images. Two and three dimensional plots were made from the digital analyses results. These plotted data greatly enhanced the GRR image content, and it was possible for nontrained observers to correctly identify normal vs abnormal ocular status by viewing the plots. Based upon the criteria of detecting equality or inequality of ocular status of a person's eyes, the trained observer correctly identified the ocular status of 90% of the 232 persons who participated in this program
A genetic assessment of parentage in the blackspot sergeant damselfish, Abudefduf sordidus (Pisces: Pomacentridae)
Microsatellite markers were used to investigate the reproductive behavior of the damselfish Abudefduf sordidus at Johnston Atoll, Central Pacific Ocean. Genetic results indicated that ten males maintained guardianship over their nest territories for up to nine nest cycles during a 3.5 month period. Genotypes of 1025 offspring sampled from 68 nests (composed of 129 clutches) were consistent with 95% of the offspring being sired by the guardian male. Offspring lacking paternal alleles at two or more loci were found in 19 clutches, indicating that reproductive parasitism and subsequent alloparental care occurred. Reconstructed maternal genotypes allowed the identification of a minimum of 74 different females that spawned with these ten territorial males. Males were polygynous, mating with multiple females within and between cycles. Genetic data from nests, which consisted of up to four clutches during a reproductive cycle, indicated that each clutch usually had only one maternal contributor and that different clutches each had different dams. Females displayed sequential polyandry spawning with one male within a cycle but switched males in subsequent spawning cycles. These results highlight new findings regarding male parasitic spawning, polygyny, and sequential polyandry in a marine fish with exclusive male paternal care.Published versio
Spectral Energy Distributions for Disk and Halo M--Dwarfs
We have obtained infrared (1 to 2.5 micron) spectroscopy for 42 halo and disk
dwarfs with spectral type M1 to M6.5. These data are compared to synthetic
spectra generated by the latest model atmospheres of Allard & Hauschildt.
Photospheric parameters metallicity, effective temperature and radius are
determined for the sample. We find good agreement between observation and
theory except for known problems due to incomplete molecular data for metal
hydrides and water. The metal-poor M subdwarfs are well matched by the models
as oxide opacity sources are less important in this case. The derived effective
temperatures for the sample range from 3600K to 2600K; at these temperatures
grain formation and extinction are not significant in the photosphere. The
derived metallicities range from solar to one-tenth solar. The radii and
effective temperatures derived agree well with recent models of low mass stars.Comment: 24 pages including 13 figures, 4 Tables; accepted by Ap
In vitro and in vivo studies of the trypanocidal properties of WRR-483 against Trypanosoma cruzi.
BackgroundCruzain, the major cysteine protease of Trypanosoma cruzi, is an essential enzyme for the parasite life cycle and has been validated as a viable target to treat Chagas' disease. As a proof-of-concept, K11777, a potent inhibitor of cruzain, was found to effectively eliminate T. cruzi infection and is currently a clinical candidate for treatment of Chagas' disease.Methodology/principal findingsWRR-483, an analog of K11777, was synthesized and evaluated as an inhibitor of cruzain and against T. cruzi proliferation in cell culture. This compound demonstrates good potency against cruzain with sensitivity to pH conditions and high efficacy in the cell culture assay. Furthermore, WRR-483 also eradicates parasite infection in a mouse model of acute Chagas' disease. To determine the atomic-level details of the inhibitor interacting with cruzain, a 1.5 A crystal structure of the protease in complex with WRR-483 was solved. The structure illustrates that WRR-483 binds covalently to the active site cysteine of the protease in a similar manner as other vinyl sulfone-based inhibitors. Details of the critical interactions within the specificity binding pocket are also reported.ConclusionsWe demonstrate that WRR-483 is an effective cysteine protease inhibitor with trypanocidal activity in cell culture and animal model with comparable efficacy to K11777. Crystallographic evidence confirms that the mode of action is by targeting the active site of cruzain. Taken together, these results suggest that WRR-483 has potential to be developed as a treatment for Chagas' disease
Astrophysical limitations to the identification of dark matter: indirect neutrino signals vis-a-vis direct detection recoil rates
A convincing identification of dark matter (DM) particles can probably be
achieved only through a combined analysis of different detections strategies,
which provides an effective way of removing degeneracies in the parameter space
of DM models. In practice, however, this program is made complicated by the
fact that different strategies depend on different physical quantities, or on
the same quantities but in a different way, making the treatment of systematic
errors rather tricky. We discuss here the uncertainties on the recoil rate in
direct detection experiments and on the muon rate induced by neutrinos from
dark matter annihilations in the Sun, and we show that, contrarily to the local
DM density or overall cross section scale, irreducible astrophysical
uncertainties affect the two rates in a different fashion, therefore limiting
our ability to reconstruct the parameters of the dark matter particle. By
varying within their respective errors astrophysical parameters such as the
escape velocity and the velocity dispersion of dark matter particles, we show
that the uncertainty on the relative strength of the neutrino and
direct-detection signal is as large as a factor of two for typical values of
the parameters, but can be even larger in some circumstances.Comment: 12 pages, 3 figures. Improved presentation and Fig.3; clarifications,
references and an appendix added; conclusions unchanged. Matches version
published in PR
The energy budget in Rayleigh-Benard convection
It is shown using three series of Rayleigh number simulations of varying
aspect ratio AR and Prandtl number Pr that the normalized dissipation at the
wall, while significantly greater than 1, approaches a constant dependent upon
AR and Pr. It is also found that the peak velocity, not the mean square
velocity, obeys the experimental scaling of Ra^{0.5}. The scaling of the mean
square velocity is closer to Ra^{0.46}, which is shown to be consistent with
experimental measurements and the numerical results for the scaling of Nu and
the temperature if there are strong correlations between the velocity and
temperature.Comment: 5 pages, 3 figures, new version 13 Mar, 200
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