EFETIVIDADE DA ASSOCIAÇÃO DO EXERCICIO FISICO E PLASMA RICO EM PLAQUETAS NA INFLAMAÇÃO ARTICULAR

O tratamento das lesões na cartilagem representam um desafio, devido a sua baixa atividade metabólica, regeneração e reação inflamatória. Investigar os efeitos do plasma rico em plaquetas na inflamação aguda e crônica da cartilagem articular, de forma isolada e em associação ao exercício terapêutico. 44 ratos Wistar divididos em 4 grupos: grupo controle (GCON), grupo tratado-plasma rico em plaquetas (GPRP), grupo tratado-exercício (GEXER) e grupo tratado-plasma rico em plaquetas e exercício (GPRP+EXER), por 3 dias (fase aguda) e 14 dias (fase crônica). A análise microscópica observou a presença ou não de células inflamatórias agudas e crônicas. Realizaram-se testes de normalidade e de significância com 3 dias todos os grupos tratados obtiveram uma diminuição da quantidade de células inflamatórias. Com 14 dias apenas o grupo GPRP+EXER, obteve uma diminuição estatisticamente significante. Na fase aguda todos os tratamentos foram efetivos, na etapa crônica a associação das terapias se mostra superior para a redução da inflamação articular

Quercetin action on pain modulation/ Ação da quercetina sobre a modulação da dor

Background: Quercetin is a flavonoid widely found in plant kingdom and target of studies in pharmacological area due to its potent antinociceptive effect compared to analgesics used in conventional therapies. The aim was to evaluate its antinociceptive activity and antinociception mechanism. Methods: For this, 40 Norvegicus Wistar rats were used, divided into 4 groups: Q50 (treated with quercetin 50 mg/Kg), Q100 (treated with quercetin 100 mg/Kg), Q500 (treated with quercetin 500 mg/Kg) and Positive control (PC) without quercetin treatment), who were submitted through the pain induction methods by tail immersion and formalin in the first step to assess antinociceptive action and in the second step, tail immersion method receiving antagonists from opioid, cholinergic and nitric oxide - L-arginine to evaluate the action mechanism. Results: Quercetin antinociceptive activity was verified at the dose of 50 mg/kg and 100 mg/kg in tail immersion test after formalin injection, with better performance at the dose of 50 mg/kg. There were no statistically significant results in paw opening and capsaicin tests. Quercetin demonstrated a possible influence on opioid and cholinergic pathway, which was not observed on the nitric acid - L-arginine pathway in view of parameters tested. Conclusion: Quercetin performed the best antinociceptive activity at a dose 50 mg/kg and there was a possible influence on opioid and cholinergic pathways

Pain neuromodulation exerted by Ruta graveolens aqueous extract in experimental models of nociception/ Neuromodulação da dor exercida pela Ruta graveolens extrato aquoso em modelos experimentais de nocicepção

Introduction: The use of medicinal plants for therapeutic purposes has been common practice since antiquity. Ruta graveolens L., commonly known as rue, has been shown to have antiparasitic, antioxidant, antibacterial and allelopathic activity. Objective: The objective was to investigate the antinociceptive effect of rue, as well as the mechanisms behind this effect. Materials and Methods: The sample consisted of 40 male Norvegicus (Wistar) rats, randomly divided into a positive control and three treatment groups administered Ruta graveolens L. aqueous extract at the following doses: 50 mg/kg, 100 mg/kg or 500 mg/kg, p.o. The experimental models of nociception used in this study to assess effectiveness of the treatments were the formalin and capsaicin tests. Five days prior to nociceptive challenges, the tail immersion assay was conducted to determine baseline pain threshold. Results: Antinociceptive activity was observed at Ruta graveolens L. aqueous extract concentrations of 50 mg/kg and 100mg/kg. 500 mg/kg induced pro-nociceptive activity with activation of the L-arginine-oxide-nitric system. Conclusion: These results suggest Ruta graveolens L. aqueous extract antinociceptive activity, and possible antagonism towards receptor

Análise quantitativa e qualitativa da neuroinflamação pela toxina beta amilóide 1-42 após tratamento com nanopartículas de resveratrol

Introdução: Este estudo avaliou os efeitos das nanopartículas de zeína com resveratrol na neuroinflamação causada pela doença de Alzheimer. Método: A amostra consistiu em 30 animais divididos em grupos de controle (C), controle positivo (CP), nanopartículas brancas (NB), nanopartículas de resveratrol (NR) e resveratrol (R). Os animaisreceberam 10 mg/kg de resveratrol ou nanopartículas de acordo com o grupo, diariamente, por 15 dias, por via oral. Em seguida, foram submetidos a análises imuno-histoquímicas (IHC). Resultados: A IHC mostrou que não houve alteração na composição morfológica do cérebro nos grupos NR e C. Por outro lado, nos grupos CP, NB e R, foram observadas alterações na deposição de Anti Tau. O grupo NR mostrou uma projeção normal de taurinano axônio, que não se apresentou da mesma forma nos outros grupos. O marcador CD68 não mostrou ativação microglial nos grupos R e C. As análises quantitativas do antibeta-amiloide no grupo NR mostraram uma diferença estatística quando comparadas aos grupos CP, NB e R, enquanto a análise do antitau mostrou uma diferença significativa entre os grupos CP e NR. O marcador CD68 mostrou uma diferença significativa entre os grupos C e NR. A análise das citocinas mostrou uma diferença significativa no TNF-α entre os grupos C e CP, C e NB, CP e NR, e NB e NR. IL-6 e InF-δ não apresentaram diferença significativa entre todos os grupos. A IL-10 apresentou diferenças significativas entre os grupos C e NR, C e R, e CP e NR. Conclusão: A NR impediu a evolução da neuroinflamação.Background: This study evaluated the effects of zein nanoparticles with resveratrol on neuroinflammation caused by Alzheimer’s disease. Method: The sample consisted of 30 animals divided into control (C), positive control (CP), white nanoparticles (NB), resveratrol nanoparticles (NR) and resveratrol (R) groups. The animals received 10 mg/kg of resveratrol or nanoparticles according to the group, daily, for 15 days, oral administration. Afterward, they weresubmitted to immunohistochemical (IHC) analyses. Results: the IHC showed that there was no change in the morphological brain composition in the NR and C groups. Conversely, in the CP, NB, and R groups, changes in the deposition of Anti Tau were observed. The NR group showed a normal projection of taurine in the axon, which was not presented in the same way in the other groups. The CD68 marker showed no microglial activation in the R and C groups. Quantitative analyses of Anti Beta-Amyloid in the NR group showed a statistical difference comparedto the CP, NB, and R groups, whereas the Anti Tau analysis showed a significant difference between the CP and NR groups. The CD68 marker showed a significant difference between the C and NR groups. The analysis of cytokines showed a significant difference in TNF-α between the C and CP groups, C and NB groups, CP and NR groups, and NB and NR groups. IL-6 and InF-δ showed no significant difference between all groups. IL-10 showed significant differences between the C and NR groups, C and R groups, and CP and NR groups. Conclusion: NR prevented the evolution of neuroinflammation

Efeito do equilíbrio neuromuscular na análise do equilíbrio estático em idosos / Effect of neuromuscular balance on static balance analysis in the elderly

Introdução: O Envelhecimento, enquanto estado orgânico é irreversível, e o equilíbrio é um dos comprometimentos adquiridos no envelhecimento, o equilíbrio neuromuscular (ENM) foi desenvolvido por François Soulier, França, após cinco anos de estudos, ao conhecer um aparelho chamado activador methods, que é uma caneta a qual gera vibrações, criado por quiropratas, nos EUA, com o objetivo de substituir as mãos na realização de uma técnica manipulativa. Metodologia: 5 idosos, com idade de 60 a 80 anos, no local de desenvolvimento da pesquisa 5 dias por semana, com uma duração de 20 minutos por atendimento. Foram realizadas as avaliações baropodométrica desses pacientes para identificar quais os locais de divisão de massa (anterior e posterior), em seguida eles receberam o tratamento de ENM, e depois a reavaliação baropodométrica. Resultados: A técnica do ENM demostrou resultados significativos pois promoveu uma melhora na descarga do peso corporal antero-posterior. Conclusão: A técnica ENM demostrou resultados significativos no tratamento de distúrbios de equilíbrio em idosos

Therapeutic Approaches for Alzheimer’s Disease: New Perspectives

Alzheimer’s disease (AD) was defined as a neurodegenerative disorder, being more affected in the elderly. It is estimated that every 3.2 seconds a person in the world is affected by the high disease that rate in 2050 to 1 second. Therefore, research has been carried out on new therapeutic approaches, such as Transcranial Photobiomodulation and treatment based on antioxidants, such as Resveratrol. Therefore, the objective is to conduct a literature review on these two approaches and their effects on the treatment of AD. It was carried out according to the PRISMA recommendation and the articles were selected according to the years of publication (between 2015 and 2020) and extracted from the following databases: Science Direct, PubMed PMC, Scopus, PubMed NCBI, SciELO, LILACS, MEDLINE and PEDro. In several studies it has been reported that both therapies provide improvements at the molecular and behavioral level, recovering brain functions, acting in a neuroprotective way, improving quality of life, with few adverse effects and in a less invasive way. Thus, both treatments have numerous benefits that can be useful in the treatment of AD. However, there is a need for further research that includes interventions with greater specificity and control, so that they are defined as ideal doses and treatment protocols