Carbon Nanosorbent for Purification Different Biomolecules

The article presents the results of physico-chemical studies on the development of nanostructured carbon materials from domestic raw materials. Were obtained and tested micro-mesoporous carbon sorbents for molecular-sieve chromatography of markers and investigated the applicability of carbon sorbents for the separation of protein-lipid complex, and plant bio-stimulator. Carbon sorbents have well-developed porous structure but their disadvantage is the weak mechanical strength. Recently it was shown that some carbon nanostructures have enormous strength. Thus arose the need to give the nano structured elements to carbon sorbent. Creating carbon sorbents containing nanocarbon structure was the aim of our study, as these by sorbents will be very useful for large-scale purification of biomolecules. The new carbon nanosorbent was prepared by carbonization of the stones of abricot seeds. The physico - chemical characteristics of nanostructured carbon sorbent was investigated by modern methods like scanning electrone microscope and infra red spectrophotometry. Based on the goal, nano-carbon materials in the laboratory of the Institute of Combustion Problems, Al-Farabi Kazakh National University synthesized nanostructured carbon sorbents – ‘Nanokarbosorb’ type for chromatography of biomolecules. It should be noted that the nanostructured elements of the frame attached to an unusually high mechanical strength to nanokarbosorb. Because of this, this sorbent can withstand high fluid pressure at work and has high durability, therefore, it can be used over the years. The sorbent has a very large porosity and large internal surface and, accordingly, a large capacity and has no parasitic sorption. Studies have shown that "Nanokarbosorb" suitable for purification of a powerful biostimulator plants. In this regard, of great interest represents nanostructured carbon sorbents with improved chromatography characteristics. Carbon sorbents known to mankind over thousands of years. They arewidely used for purification of alcohol and other solutions. They are mechanically very weak and quickly attacked by fungi and bacteria. In this reason they are unconvenient large scale purification biomolecules. However, further improvement of chromatographic sorbents is impossible without the use of ideas and techniques of nanotechnology

Sorption-Based Removal Techniques for Microplastic Contamination of Tap Water

[EN] This study investigates the presence of microplastics in tap drinking water and evaluates the e¿cacy of various sorbents for their removal in the context of Kazakhstan¿s water treatment system. Water samples taken in the cities of Kokshetau and Krasny Yar (Akmola region) were analyzed. Microplastics were detected in all samples, with concentrations ranging from 2.0 × 10 ¿2 to 6.0 × 10 ¿2 particles/dm3 , predominantly in ¿ber form (74.1%). Outdated technologies and non-compliance with treatment regimens contribute to poor water quality, including high turbidity (87% of samples), color deviations (40% of samples), and acidity issues (20% of samples). To address these challenges, the study examined the sorption e¿ciency of di¿erent sorbents, with results indicating high retention rates (82.7¿97.8%) for microplastic particles. Notably, aliphatic structures like PE and PP exhibited higher retention than PET. Among the sorbents tested, the synthesized carbon sorption material (CSM) demonstrated the highest e¿ciency in both microplastic retention and improvement in water quality parameters, making it a promising option for water treatment facilities and household ¿lters.This research was funded by the Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan through the research project Health risk modelling based on the identification of microplastics in water systems and the reasoning about actions to manage the water resources quality (grant AP14869081).Salikova, NS.; Kerimkulova, AR.; Rodrigo-Ilarri, J.; Alimova, KK.; Rodrigo-Clavero, M.; Kapbassova, GA. (2024). Sorption-Based Removal Techniques for Microplastic Contamination of Tap Water. Water. 16(10). https://doi.org/10.3390/w16101363161

Structure Formation of the Surface Layer of Soil as a Way to Prevent a Wind and Water Erosion

The present work describes the results of a study of structuring ability of water-soluble polymers such as polydimethyl diallyl ammonium chloride, saponification product of polyacrylonitrile (uniflok) and their interpolymer complex. On the kinetics of growth of Rehbinder’s plastic durability of coagulation structure of soil concentrated suspensions the effect of water soluble polymers and interpolymer complexes on the structure formation in the dispersions was studied. It was determined that the process is a two-step process which was caused by the adsorption step process: the binding of macromolecules with the soil surface by the random segments and conformational changes of macromolecules in the adsorbed layer. It was found that the soil stability structured by polymers against wind and water erosion increased with the concentration of water-soluble polymers. This is due to the increase of the structure formation in concentrated suspensions of soil in the presence of water soluble polymers. On the basis of the results it can be concluded that the treatment of mineral dispersions by water-soluble polymers alone or in the form of the interpolymer complex leads to a significant reduction of dust-and run-off of soil particles. The PDMDAAC/uniflok interpolymer complex in comparison with polymers reduces intensively the deflation which has a great economic and ecological importance

An association between TRP64ARG polymorphism of the B3 adrenoreceptor gene and some metabolic disturbances

<p>Abstract</p> <p>Backgrounds</p> <p>B3 adrenoreceptors (ADRB3) are abundant in adipose tissue and play the role in its metabolism and lipolysis. Some variants of the ADRB3 gene may predispose subjects for the development obesity and metabolic abnormalities in the setting of modern sedentary lifestyle. ADRB3 gene polymorphism association with metabolic disturbances has never been studied before in the ethnic Kyrgyz population.</p> <p>Aim</p> <p>To study an association between Trp64Arg polymorphism of the ADRB3 and metabolic syndrome (MS) components in an ethnic Kyrgyz group.</p> <p>Materials and methods</p> <p>213 Ethnic Kyrgyz volunteers over the age of 30 were enrolled in the study. The assessment plan for each individual comprised of general physical and anthropometric exams as well as laboratory tests (glucose, lipid panel, insulin) and genotyping by Trp64Arg polymorphism of the ADRB3. MS diagnosis was consistent with modified ATP III criteria (2005). Logistic regression analysis was performed to test the potential independent association between Arg64 allele with obesity, abdominal obesity (AO) and arterial hypertension (AH).</p> <p>Results</p> <p>Trp64Arg polymorphism of the ADRB3 was assessed in 213 individuals (145 men, 68 women) aged 30-73 (mean age 50.7 ± 7.6). Arg64 allele frequency was 0.239; ADRB3 genotype distribution among participants was: Trp64 homozygotes 54.5%, Trp64Arg 43.2% and Arg64 homozygotes 2.3%. There was an association between Trp64Arg и Arg64Arg genotypes and higher BMI, WC and obesity frequency (p < 0.00009), AO (p < 0.01), type 2 diabetes mellitus (DM) (p < 0.005) and lower high density cholesterol (HDL-C) level (p < 0.03). The logistic regression analysis showed the correlation of the Arg64 allele with obesity (OR 3.159; 95% CI 1.789-5.577) and AO (OR 1.973; 95% CI 1.118-3.481). The association between Arg64 allele and AH lost its significance after adjustment for obesity.</p> <p>Conclusion</p> <p>Arg64 allele of the ADRB3 gene in the studied group has an association with MS components such as obesity, AO and decreased HDL-C level.</p

Self-Supporting Hybrid Supercapacitor Electrodes Based on Carbon Nanotube and Activated Carbons

Self-supporting AC (activated carbon)-FWCNT (few-wall carbon nanotubes) hybrid electrodes were fabricated by mixing of ACs with high specific surface area (SSA) and sub-millimeter-long FWCNTs. In order to fabricate the hybrid electrodes, AC and FWCNT were mixed in a weight ratio of 9:1, dispersed by bath-sonication and vacuum-filtrated on a membrane filter. The addition of FWCNTs gives conductivity and mechanical strength, and replace metallic current collectors in thick (0.1 mm) electrodes. For making an electrode, three different ACs that derived from walnut shell (WS), that from apricot stones (AS), and that commercially used for capacitors (YP-80F, Kuraray Chemical Co., Osaka Japan), were used with FWCNT in weight ratio of AC:FWCNT = 9:1. An electrode based only on FWCNT was also prepared as a reference for comparison. Electrochemical properties of the obtained electrodes were investigated by the cyclic voltammetry method (CV). Electrochemical characteristics were measured using the three-electrode cell contained of YP-80F-FWCNT, AS-FWCNT, WS-FWCNT as a working electrode, a YP-80F-FWCNT counter electrode and a Ag/AgCl reference electrode with an electrolyte of 1 M Na2SO4 aqueous solution. Also, the morphological properties of obtained electrodes were studied using scanning electron microscope (SEM), the SSA was investigated by the Brunauer-Emmett-Teller analysis. SSA, conductivity, and resistivity of AS-FWCNT and WS-FWCNT electrodes were summarized. Both the AS-FWCNT and WS-FWCNT hybrid electrodes showed specific capacitances of about 140 F/g at 1 mV/s and about 100 F/g at 100 mV/s, which are similar or even better than the AC-CNT hybrid electrode made of commercialized AC (YP-80F)

Combustion Characteristics of HAN-based Green Propellant Assisted with Nanoporous Active Carbons

Combustion of hydroxylammonium nitrate (95 wt.% HAN) ‒ water solution in presence of high specific surface area activated carbons is investigated in a constant-pressure bomb within the pressure range of 1‒6 MPa. The linear burning rate increased for the system of HAN admixed with activated carbons compared to those of the HAN alone. Moreover, the thermal decomposition of HAN (95 wt.%) ‒ water solution spiked with activated carbons was assessed by DTA – TG method. In the presence of activated carbons, the ability to trigger the decomposition at a lower temperature (86 °C vs 185 °C) was observed. The volatile products formed in the course of thermal decomposition of HAN, spiked with activated carbons were characterized by electron ionization mass spectrometry analysis. Primary products of HAN decomposition: m/z = 33 (NH2OH) and m/z = 63 (HNO3), which are further responsible for the formation of secondary products such as N2O, NO, HNO2, NO2, O2 etc. Significant reduction of NOx emissions during thermal decomposition of HAN (95 wt.%) ‒ water solution was observed (ca. 30%) in presence of activated carbons

Comparison of different classification of metabolic syndrome in the Kyrgyz ethnic group

Goal: Comparison of different classification of metabolic syndrome (MS) in Kyrgyz ethnic group. Materials and methods: 183 women, 140 men of the ethnic Kyrgyz, mean age 52,5 ± 8,5 years, were included in the study. Clinical examination, blood pressure, anthropometric data (weight, height, waist circumference, hip circumference), measurament, the analiysis of fasting glucose, lipid profile, insulin were perfomed in all examined persons. Diagnosis of MS was made using the criteria of ATP III (2005), IDF for European and asian populations (2005), Russian criteria (2009) and criteria of MS IDF and AHA / NHLBI (2009). Results: The sensitivity of detection of insulin resistance (IR) was high enough and did not differ significantly between the diagnostic criteria. The highest specificity 0,77 (0,72-0,83) occurred when criteria ATP III was used. The difference of specificity was close to significant between criterias of ATP III and IDF criteria for Asian populations and criteria MS IDF and AHA / NHLBI (2009), and reached statistical significance between the parameters of the ATP III, and Russian criteria. Conclusion: Based on these data, in Kyrgyz ethnic group for diagnosis of MS is preferebale the use of ATP III criteria

Nanostructured Carbon Materials for Biomedical Use

One of the priority trends of carbon nanotechnology is creation of nanocomposite systems. Such carbon nanostructured composites were produced using - raw materials based on the products of agricultural waste, such as grape stones, apricot stones, rice husk. These products have a - wide spectrum of application and can be obtained in large quantities. The Institute of Combustion Problems has carried out the work on synthesis of the nanostructured carbon sorbents for multiple applications including the field of biomedicine. The article presents the data on the synthesis and physico-chemical properties of carbonaceous sorbents using physicochemical methods of investigation: separation and purification of biomolecules; isolation of phytohormone - fusicoccin; adsorbent INGO-1 in the form of an adsorption column for blood detoxification, oral (entero) sorbent - INGO-2; the study of efferent and probiotic properties and sorption activity in regard to the lipopolysaccharide (LPS), new biocomposites - based on carbonized rice husk (CRH) and cellular microorganisms; the use of CRH in wound treatment. A new material for blood detoxication (INGO-1) has been obtained. Adsorption of p-cresyl sulfate and indoxyl sulfate has shown that active carbon adsorbent can remove clinically significant level of p-cresyl sulfate and indoxyl sulfate from human plasma. Enterosorbent INGO-2 possesses high adsorption activity in relation to Gram-negative bacteria and their endotoxins. INGO-2 slows down the growth of conditionally pathogenic microorganisms, without having a negative effect on bifido and lactobacteria. The use of enterosorbent INGO-2 for sorption therapy may provide a solution to a complex problem - detoxication of the digestive tract and normalization of the intestinal micro ecology. The immobilized probiotic called "Riso-lact" was registered at the Ministry of Health of the Republic of Kazakhstan as a biologically active food additive. The developed technology is patented and provides production of the medicine in the form of freeze-dried biomass immobilized in vials

Impact of clinical phenotypes on management and outcomes in European atrial fibrillation patients : a report from the ESC-EHRA EURObservational Research Programme in AF (EORP-AF) General Long-Term Registry

Background: Epidemiological studies in atrial fibrillation (AF) illustrate that clinical complexity increase the risk of major adverse outcomes. We aimed to describe European AF patients’ clinical phenotypes and analyse the differential clinical course. Methods: We performed a hierarchical cluster analysis based on Ward’s Method and Squared Euclidean Distance using 22 clinical binary variables, identifying the optimal number of clusters. We investigated differences in clinical management, use of healthcare resources and outcomes in a cohort of European AF patients from a Europe-wide observational registry. Results: A total of 9363 were available for this analysis. We identified three clusters: Cluster 1 (n = 3634; 38.8%) characterized by older patients and prevalent non-cardiac comorbidities; Cluster 2 (n = 2774; 29.6%) characterized by younger patients with low prevalence of comorbidities; Cluster 3 (n = 2955;31.6%) characterized by patients’ prevalent cardiovascular risk factors/comorbidities. Over a mean follow-up of 22.5 months, Cluster 3 had the highest rate of cardiovascular events, all-cause death, and the composite outcome (combining the previous two) compared to Cluster 1 and Cluster 2 (all P &lt;.001). An adjusted Cox regression showed that compared to Cluster 2, Cluster 3 (hazard ratio (HR) 2.87, 95% confidence interval (CI) 2.27–3.62; HR 3.42, 95%CI 2.72–4.31; HR 2.79, 95%CI 2.32–3.35), and Cluster 1 (HR 1.88, 95%CI 1.48–2.38; HR 2.50, 95%CI 1.98–3.15; HR 2.09, 95%CI 1.74–2.51) reported a higher risk for the three outcomes respectively. Conclusions: In European AF patients, three main clusters were identified, differentiated by differential presence of comorbidities. Both non-cardiac and cardiac comorbidities clusters were found to be associated with an increased risk of major adverse outcomes

Impact of renal impairment on atrial fibrillation: ESC-EHRA EORP-AF Long-Term General Registry

Background: Atrial fibrillation (AF) and renal impairment share a bidirectional relationship with important pathophysiological interactions. We evaluated the impact of renal impairment in a contemporary cohort of patients with AF. Methods: We utilised the ESC-EHRA EORP-AF Long-Term General Registry. Outcomes were analysed according to renal function by CKD-EPI equation. The primary endpoint was a composite of thromboembolism, major bleeding, acute coronary syndrome and all-cause death. Secondary endpoints were each of these separately including ischaemic stroke, haemorrhagic event, intracranial haemorrhage, cardiovascular death and hospital admission. Results: A total of 9306 patients were included. The distribution of patients with no, mild, moderate and severe renal impairment at baseline were 16.9%, 49.3%, 30% and 3.8%, respectively. AF patients with impaired renal function were older, more likely to be females, had worse cardiac imaging parameters and multiple comorbidities. Among patients with an indication for anticoagulation, prescription of these agents was reduced in those with severe renal impairment, p&nbsp;&lt;.001. Over 24&nbsp;months, impaired renal function was associated with significantly greater incidence of the primary composite outcome and all secondary outcomes. Multivariable Cox regression analysis demonstrated an inverse relationship between eGFR and the primary outcome (HR 1.07 [95% CI, 1.01–1.14] per 10&nbsp;ml/min/1.73&nbsp;m2 decrease), that was most notable in patients with eGFR &lt;30&nbsp;ml/min/1.73&nbsp;m2 (HR 2.21 [95% CI, 1.23–3.99] compared to eGFR ≥90&nbsp;ml/min/1.73&nbsp;m2). Conclusion: A significant proportion of patients with AF suffer from concomitant renal impairment which impacts their overall management. Furthermore, renal impairment is an independent predictor of major adverse events including thromboembolism, major bleeding, acute coronary syndrome and all-cause death in patients with AF