PSD-95 Is Associated with the Postsynaptic Density and Not with the Presynaptic Membrane at Forebrain Synapses

PSD-95, a prominent protein component of the postsynaptic density (PSD) fraction from rat forebrain, has been localized by light microscopy to dendrites of hippocampal neurons (Cho et al., 1992) and to the presynaptic plexus of cerebellar basket cells (Kistner et al., 1993). Here we extend these studies to show that an affinity-purified antibody to PSD-95 labels the dendrites of most neurons in the forebrain and of a subset of neurons in the cerebellum. To confirm that PSD-95 is associated with the PSD at forebrain synapses and to clarify whether it is also associated with the presynaptic membrane, we employed immunogold electron microscopy of forebrain synaptosomes. Gold-labeled antibodies to PSD-95 labeled postsynaptic densities in both intact and lysed forebrain synaptosomes but did not label presynaptic terminals or the presynaptic membrane. The asymmetric distribution of PSD-95 at synapses contrasts with that of its homologs, disks-large and ZO-1, which are arranged symmetrically at septate and tight junctions, respectively

Mapping food access and related risk factors in forecasting social disorder

Includes bibliographical references

Situating Open Educational Resources for CSU Faculty and Student Success

Economic inequity is a pervasive aspect of the population of California State University (CSU) students. There are significant differences in the ability of our students to afford the total cost of their CSU education. Not only are they afforded less access to educational materials, but they also may be disadvantaged in knowing how to take advantage of those resources. Providing free, open, and/or low-cost options for course materials is a CSU Affordable Learning Solutions (AL$) strategy to significantly reduce the impact of economic inequities. Thus, we situate open as enabling equitable academic opportunities and successful educational outcomes for all CSU students. Through state supported legislation and grant funding, AL$ has built the California Online Open Library for Education (www.cool4ed.org) with open textbooks for 52 courses articulated across California’s higher education systems. We have partnered with legislators, the Hewlett and Gates Foundation, the Community Colleges, and the University of California and acquired $5M for COOL4Ed.org and for faculty development programs. By 2020, we anticipate that COOL4Ed will expand its open textbook catalog from 52 to 150 courses. In this session Drs. Leslie Kennedy and Lesley Farmer will provide an overview of a variety of open education resources showcasing CSU faculty and student experiences with OER. In addition, they will review the current status of the CSU OER initiatives and strategies for future expansion in support of faculty instruction and student learning success

C-Terminal truncation of NR2A subunits impairs synaptic but not extrasynaptic localization of NMDA receptors

NMDA receptors interact via the extended intracellular C-terminal domain of the NR2 subunits with constituents of the postsynaptic density for purposes of retention, clustering, and functional regulation at central excitatory synapses. To examine the role of the C-terminal domain of NR2A in the synaptic localization and function of NR2A-containing NMDA receptors in hippocampal Schaffer collateral–CA1 pyramidal cell synapses, we analyzed mice which express NR2A only in its C-terminally truncated form. In CA1 cell somata, the levels, activation, and deactivation kinetics of extrasynaptic NMDA receptor channels were comparable in wild-type and mutant NR2A^(ΔC/ΔC) mice. At CA1 cell synapses, however, the truncated receptors were less concentrated than their full-length counterparts, as indicated by immunodetection in cultured neurons, synaptosomes, and postsynaptic densities. In the mutant, the NMDA component of evoked EPSCs was reduced in a developmentally progressing manner and was even more reduced in miniature EPSCs (mEPSCs) elicited by spontaneous glutamate release. Moreover, pharmacologically isolated NMDA currents evoked by synaptic stimulation had longer latencies and displayed slower rise and decay times, even in the presence of an NR2B-specific antagonist. These data strongly suggest that the C-terminal domain of NR2A subunits is important for the precise synaptic arrangement of NMDA receptors

CCTV as a Tool for Early Police Intervention: Preliminary Lessons from Nine Case Studies.

This study explores the prospect of utilizing CCTV as an early intervention mechanism to detect and disrupt street-level activity that can lead to violence. The analysis focuses on nine case studies in Newark, NJ, incorporating data from several sources, including video footage, computer-aided dispatch (CAD) system incident data, CAD event chronologies and face-to-face interviews with CCTV operators. The findings suggest that the benefits offered by CCTV, namely the instantaneous discovery and reporting of crime, may be rendered incon- sequential by the process times associated with the differential-response policy of police dispatch. Potential methods by which police can more proactively utilize CCTV to prevent crime are discussed

Joint Utility of Event- Dependent and Environmental Crime Analysis Techniques for Violent Crime Forecasting

Violent crime incidents occurring in Irvington, New Jersey, in 2007 and 2008 are used to assess the joint analytical capabilities of point pattern analysis, hotspot mapping, near-repeat analysis, and risk terrain modeling. One approach to crime analysis suggests that the best way to predict future crime occurrence is to use past behavior, such as actual incidents or collections of incidents, as indicators of future behavior. An alternative approach is to consider the environment in which crimes occur and identify features of the landscape that would be conducive to crime. Thanks to advances in geographic information system technology and federally funded (free) software applications such as CrimeStat III or the Near Repeat Calculator, these methods have recently been made more accessible to “average” users. This study explores the information products that each method offers for the purposes of place-based violent crime forecasting and resource allocation. Findings help to answer questions about where, when, and why violent crimes occur in a jurisdiction. Ways in which event-dependent and environmental crime analysis techniques can be utilized as complementary instruments in a crime analyst’s tool kit are discussed in detail

Uncleared Homicides: A Canada/United States Comparison

Beginning in the 1960s, there has been a marked decline in clearance rates of homicides, a finding that has generated little interest among criminological researchers. This article presents a comparative analysis of homicide clearance in Canada and the United States using data generated by the Canadian Centre of Justice Statistics and the U.S. Federal Bureau of Investigation\u27s Supplementary Homicide Reports. Using logistic regression, homicide clearance is predicted on the basis of specific victim and offense characteristics for cases in Canada versus the United States and in Ontario versus New York State. The results indicate that the model is a good fit for homicide clearance in both countries as a whole. Whereas the homicide weapon, circumstances surrounding the offense, age, and gender of the victim were found to be significant homicide clearance predictors in New York State, only the circumstances surrounding the offense emerged as an important predictor in Ontario

Place-based correlates of Motor Vehicle Theft and Recovery: Measuring spatial influence across neighbourhood context

Social scientists have long shown great interest in the spatial correlates of crime patterns. A subset of the literature has focused on how micro-level spatial factors influence the formation of crime hot spots. At the same time, tangential research has highlighted how neighbourhood disadvantage influences crime occurrence. The current study focuses on the intersection of these perspectives through a spatial analysis of Motor Vehicle Theft (MVT) and Motor Vehicle Recovery (MVR) in Colorado Springs, CO. We begin by conducting a Risk Terrain Modelling analysis to identify spatial risk factors significantly related to MVT and MVR occurrence. We then test whether the spatial influences of the criminogenic risk factors differ across traditional measures of neighbourhood disadvantage. Findings suggest that while a citywide effect is evident for multiple risk factors, their spatial influence on crime significantly varies across neighbourhood contexts