Spatiotemporal Properties of the Action Potential Propagation in the Mouse Visual Cortical Slice Analyzed by Calcium Imaging

The calcium ion (Ca2+) is an important messenger for signal transduction, and the intracellular Ca2+ concentration ([Ca2+]i) changes in response to an excitation of the cell. To reveal the spatiotemporal properties of the propagation of an excitatory signal with action potentials in the primary visual cortical circuit, we conducted a Ca2+ imaging study on slices of the mouse visual cortex. Electrical stimulation of layer 4 evoked [Ca2+]i transients around the stimulus electrode. Subsequently, the high [Ca2+]i region mainly propagated perpendicular to the cortical layer (vertical propagation), with horizontal propagation being restricted. When the excitatory synaptic transmission was blocked, only weak and concentric [Ca2+]i transients were observed. When the action potential was blocked, the [Ca2+]i transients disappeared almost completely. These results suggested that the action potential contributed to the induction of the [Ca2+]i transients, and that excitatory synaptic connections were involved in the propagation of the high [Ca2+]i region in the primary visual cortical circuit. To elucidate the involvement of inhibitory synaptic connections in signal propagation in the primary visual cortex, the GABAA receptor inhibitor bicuculline was applied. In this case, the evoked signal propagated from layer 4 to the entire field of view, and the prolonged [Ca2+]i transients were observed compared with the control condition. Our results suggest that excitatory neurons are widely connected to each other over the entire primary visual cortex with recurrent synapses, and inhibitory neurons play a fundamental role in the organization of functional sub-networks by restricting the propagation of excitation signals

Management of granulomatous lobular mastitis: an international multidisciplinary consensus (2021 edition)

Granulomatous lobular mastitis (GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence. Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions, etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology. The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidence-based consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM.Improving the Ability of Diagnosis and Treatment of Difficult Disease

The Ninth Visual Object Tracking VOT2021 Challenge Results

acceptedVersionPeer reviewe

Stimuli-Directed Helical Chirality Inversion and Bio-Applications

Helical structure is a sophisticated ubiquitous motif found in nature, in artificial polymers, and in supramolecular assemblies from microscopic to macroscopic points of view. Significant progress has been made in the synthesis and structural elucidation of helical polymers, nevertheless, a new direction for helical polymeric materials, is how to design smart systems with controllable helical chirality, and further use them to develop chiral functional materials and promote their applications in biology, biochemistry, medicine, and nanotechnology fields. This review summarizes the recent progress in the development of high-performance systems with tunable helical chirality on receiving external stimuli and discusses advances in their applications as drug delivery vesicles, sensors, molecular switches, and liquid crystals. Challenges and opportunities in this emerging area are also presented in the conclusion

Effect of lubricants on microstructure and properties of metal abrasive tools via wet molding

To study the microstructure and the thermodynamic properties of lubricants, as well as their effects on the structure and mechanical property of wet-forming or sintered materials, different lubrication release agents are added into metal abrasive tools which are fabricated with wet forming and degreasing sintering process. Synchronous thermal analyzer, scanning electron microscope and X-ray diffractometer are used to analyze the performance of the samples. The results indicate that the the residual degreasing amount of zinc stearate is about 20.00% while that of HV1 lubricant is only 3.00%. The residue of zinc stearate is the aggregate of nanometer particles and the counterpart of HV1 is ～10 μm interlocking irregular particles. Both HV1 and zinc stearate lubricant have better release effect on wet molding. However, adding HV1 or zinc stearate lubricant reduces the density of the compact by 0.5% or 5%, the density of sintered samples by 1.5% or 3.4%, and the bending strength by 4.4% or 9.1%, respectively. Compared with zinc stearate lubricant, HV1 has good demoulding effect and less influence on the properties of the compact and the sample, which is more suitable for metal abrasives of wet forming applications

Circularly polarized light modulated supramolecular self-assembly for an azobenzene-based chiral gel

UV light-triggered trans-to-cis isomerization of azobenzene usually results in the collapse of a self-assembly system owing to the breaking of molecular planarity. Interestingly, two totally opposite self-assembly trends have been detected when a C-2v-symmetric chiral gelator was irradiated by a circularly polarized light (CPL) with specific handedness, indicating that CPL could become a powerful tool in modulating the assembly behaviour of the photo-responsive system