V1: Targeting and Scaling Out

Numerous pilot studies and case studies in the Volta Basin have evaluated practices, methods, and tools that could prove beneficial to others, both within the basin and outside of it. However, the question whether an intervention successfully applied in one location has a reasonable chance of success at any other location remains extremely difficult to answer. A consistent finding in pilot studies is that detailed characteristics of the study location – economic, biophysical, institutional, and cultural – can all play an essential role in the eventual success, and failure of achieving a successful outcome. For out-scaling of initiatives it is impractical to collect detailed information at every potential site where an agricultural land and water management (AWM) intervention might be introduced. This project starts with the premise that, while certainty is unobtainable, degrees of certainty are both obtainable, using available information in a systematic way, and useful.The CPWF Project V1, “Targeting and Scaling Out”, proposal aims to develop an evidence and knowledge-based tool that will map the likelihood that a given intervention will be successful in given locations. The proposed V1 project will contribute to achieving the BDC challenge of improving rainwater and small reservoir management to contribute to poverty reduction and improved livelihoods resilience by producing a framework and web-based and electronic “decision support”, (or targeting and scaling out tool) that will identify likely sites to introduce AWM interventions for smallholder farming systems

Regulatory frameworks can facilitate or hinder the potential for Genome Editing to contribute to sustainable agricultural development

The advent of new breeding techniques (NBTs), in particular genome editing (GEd), has provided more accurate and precise ways to introduce targeted changes in the genome of both plants and animals. This has resulted in the use of the technology by a wider variety of stakeholders for different applications in comparison to transgenesis. Regulators in different parts of the world are now examining their current frameworks to assess their applicability to these NBTs and their products. We looked at how countries selected from a sample of geographical regions globally are currently handling applications involving GEd organisms and what they foresee as opportunities and potential challenges to acceptance of the technology in their jurisdictions. In addition to regulatory frameworks that create an enabling environment for these NBTs, acceptance of the products by the public is vitally important. We, therefore, suggest that early stakeholder engagement and communication to the public be emphasized to foster public acceptance even before products are ready for market. Furthermore, global cooperation and consensus on issues cutting across regions will be crucial in avoiding regulatory-related bottlenecks that affect global trade and agriculture

Evidence for genes controlling resistance to Heligmosomoides bakeri on mouse chromosome 1

Resistance to infections with Heligmosomoides bakeri is associated with a significant quantitative trait locus (QTL–Hbnr1) on mouse chromosome 1 (MMU1). We exploited recombinant mice, with a segment of MMU1 from susceptible C57Bl/10 mice introgressed onto MMU1 in intermediate responder NOD mice (strains 1094 and 6109). BALB/c (intermediate responder) and C57Bl/6 mice (poor responder) were included as control strains and strain 1098 (B10 alleles on MMU3) as NOD controls. BALB/c mice resisted infection rapidly and C57Bl/6 accumulated heavy worm burdens. Fecal egg counts dropped by weeks 10–11 in strain 1098, but strains 1094 and 6109 continued to produce eggs, harbouring more worms when autopsied (day 77). PubMed search identified 3 genes (Ctla4, Cd28, Icos) as associated with ‘Heligmosomoides’ in the B10 insert. Single nucleotide polymorphism (SNP) differences in Ctla4 could be responsible for regulatory changes in gene function, and a SNP within a splice site in Cd28 could have an impact on function, but no polymorphisms with predicted effects on function were found in Icos. Therefore, one or more genes encoded in the B10 insert into NOD mice contribute to the response phenotype, narrowing down the search for genes underlying the H. bakeri resistance QTL, and suggest Cd28 and Ctla4 as candidate genes

L1: Targeting and scaling out

Despite hosting some of the most developed sub Saharan countries, a majority of rural smallholder farmers in the Limpopo basin still live in poverty. The challenge of low and highly variable rainfall together with inadequate technology transfers, inadequate policy and investment context all act to disable successful transitions out of poverty. The CPWF Phase I identified several opportunities to manage rainfall in more efficient and productive manners at field to basin scales. The challenge of successful targeting and scaling out is still a key research and development area to contribute towards the Limpopo development challenges with opportunities to enable transformations of rural livelihoods at a greater scale. The project L1 ”Targeting and scaling out” aims to develop an evidence and knowledge-based tool that will map the likelihood that a given intervention will be successful in given locations. The tool would be intended for non-expert users and would be available via the World Wide Web. The proposed L1 project will contribute to achieving the BDC challenge of improving rainwater and small reservoir management to contribute to poverty reduction and improved livelihoods resilience. It will do this by producing a framework and web-based and electronic “decision support”, (or targeting and scaling out tool) that will identify likely sites to introduce AWM interventions for smallholder farming systems

Genetic Analysis of Reproductive Traits and Antibody Response in PRRS Infected Sows

The genetic components of reproductive performance and antibody response of 641 commercial sows were assessed in a commercial herd that faced a PRRS outbreak. Antibody response after the PRRS outbreak was highly heritable and had high genetic correlations with reproductive traits. Many genomic regions were associated with antibody response in this study. These results indicate that there is a significant genomic component associated with PRRS antibody response and its high genetic correlations with reproductive traits during PRRS suggest that this trait could be used as an indicator trait to reduce the impact of PRRS on reproductive performance

Sex differences influencing micro- and macrovascular endothelial phenotype in vitro

Key points: Endothelial dysfunction is an early hallmark of multiple disease states that also display sex differences with respect to age of onset, frequency and severity. Results of in vivo studies of basal and stimulated microvascular barrier function revealed sex differences that are difficult to ascribe to specific cells or environmental factors. The present study evaluated endothelial cells (EC) isolated from macro- and/or microvessels of reproductively mature rats under the controlled conditions of low-passage culture aiming to test the assumption that EC phenotype would be sex independent. The primary finding was that EC, regardless of where they are derived, retain a sex-bias in low-passage culture, independent of varying levels of reproductive hormones. The implications of the present study include the fallacy of expecting a universal set of mechanisms derived from study of EC from one sex and/or one vascular origin to apply uniformly to all EC under unstimulated conditions, and no less in disease. Abstract: Vascular endothelial cells (EC) are heterogeneous with respect to phenotype, reflecting at least the organ of origin, location within the vascular network and physical forces. As an independent influence on EC functions in health or aetiology, susceptibility, and progression of dysfunction in numerous disease states, sex has been largely ignored. The present study focussed on EC isolated from aorta (macrovascular) and skeletal muscle vessels (microvascular) of age-matched male and female rats under identical conditions of short-term (passage 4) culture. We tested the hypothesis that genomic sex would not influence endothelial growth, wound healing, morphology, lactate production, or messenger RNA and protein expression of key proteins (sex hormone receptors for androgen and oestrogens α and β; platelet endothelial cell adhesion molecule-1 and vascular endothelial cadherin mediating barrier function; αvβ3 and N-cadherin influencing matrix interactions; intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 mediating EC/white cell adhesion). The hypothesis was rejected because the EC origin (macro- vs. microvessel) and sex influenced multiple phenotypic characteristics. Statistical model analysis of EC growth demonstrated an hierarchy of variable importance, recapitulated for other phenotypic characteristics, with predictions assuming EC homogeneity \u3c sex \u3c vessel origin \u3c sex and vessel origin. Furthermore, patterns of EC mRNA expression by vessel origin and by sex did not predict protein expression. Overall, the present study demonstrated that accurate assessment of sex-linked EC dysfunction first requires an understanding of EC function by position in the vascular tree and by sex. The results from a single EC tissue source/species/sex cannot provide universal insight into the mechanisms regulating in vivo endothelial function in health, and no less in disease