Impaired Intracellular Transport and Cell Surface Expression of Nonpolymorphic HLA-E

The assembly of the classical, polymorphic major histocompatibility complex class I molecules in the endoplasmic reticulum requires the presence of peptide ligands and ~2-microglobulin (~2m). Formation of this trimolecular complex is a prerequisite for e~cient transport to the cell surface, where presented peptides are scanned by T lymphocytes. The function of the other class I molecules is in dispute. The human, nonclassical class I gene, HLA-E, was found to be ubiquitously transcribed, whereas cell surface expression was dif~cult to detect upon transfection. Pulse chase experiments revealed that the HLA-E heavy chain in transfectants, obtained with the murine myeloma cell line P3X63-Ag8.653 (X63), displays a significant reduction in oligosaccharide maturation and intracellular transport compared with HLA-B27 in corresponding transfectants. The accordingly low HLA-E cell surface expression could be significantly enhanced by either reducing the culture temperature or by supplementing the medium with human ~2m, suggesting inefficient binding of endogenous peptides to HLA-E. To analyze whether HLA-E binds peptides and to identify the corresponding ligands, fractions of acid-extracted material from HLA-E/X63 transfectants were separated by reverse phase HPLC and were tested for their ability to enhance HLA-E cell surface expression. Two fractions specifically increased the HLA class I expression on the HLA-E transfectant clone

The Shroud Around the Twin Radio Jets in NGC 1052

(Abridged) We discuss multiple VLBI continuum and spectral line observations and WSRT spectroscopy of NGC 1052. Sub-parsec scale features move outward at approximately 0.26c in bi-symmetric jets, most likely oriented near the plane of the sky. Absorption and emission signatures reveal ionised, atomic, and molecular components of the surrounding medium. Seven-frequency (1.4 to 43 GHz) VLBA observations show free-free absorption in the inner parsec, probably together with synchrotron self-absorption. There is apparently a geometrically thick but patchy structure oriented roughly orthogonal to the jets. The western jet is receding: it is covered more deeply and extensively. HI spectral line VLBI reveals atomic gas in front of both jets. There appear to be three velocity systems. The deepest, at "high velocities" (receding by 125 to 200 km/s), seems restricted to a shell 1 to 2 pc away from the core, within which this gas might be largely ionised. WSRT spectroscopy has revealed 1667 and 1665 MHz OH absorption with their line ratio varying roughly from 1:1 to 2:1 between -35 and 200 km/s. In the high velocity system the OH profiles are similar to HI, suggesting co-location of that atomic and molecular gas, and leaving unclear the connection to the H2O masing gas seen elsewhere. We have also detected both 18cm OH satellite lines in the high velocity system. They have conjugate profiles: 1612 MHz is in absorption, and 1720 MHz in emission.Comment: 16 pages, 14 figures, LaTeX, includes aa.cls, accepted for publication in Astronomy and Astrophysic

The shroud around the "compact, symmetric" radio jets in NGC 1052

This is a paper on young jet material in a frustratingly complex environment. NGC 1052 has a compact, flat- or GHz-peaked-spectrum radio nucleus consisting of bi-symmetric jets, oriented close to the plane of the sky. Many features on both sides move away at v_app~0.26c (H_0=65 km/s/Mpc). VLBI at seven frequencies shows a wide range of spectral shapes and brightness temperatures; there is clearly free-free absorption, probably together with synchrotron self-absorption, on both sides of the core. The absorbing structure is likely to be geometrically thick and oriented roughly orthogonal to the jets, but it is patchy. HI VLBI shows atomic gas in front of the approaching as well as the receding jet. There appear to be three velocity systems, at least two of which are local to the AGN environment. The "high velocity system", 125 to 200 km/s redward of systemic, seems restricted to a shell 1 to 2 pc away from the core. Closer to the centre, this gas might be largely ionised; it could cause the free-free absorption. WSRT spectroscopy shows 1667 and 1665 MHz OH absorption over a wide velocity range. OH and HI profile similarity suggests co-location of molecular and atomic ``high velocity'' gas; the connection to H2O masing gas is unclear. Further, at ``high velocity'' we detected the OH 1612 MHz satellite line in absorption and the 1720 MHz line in emission, with complementary strengths.Comment: 6 pages, 3 figures, prepared in LaTeX. Revised version, will appear in Publications of the Astronomical Society of Australia, as part of the proceedings of the 3rd GPS/CSS workshop, ed. T. Tzioumis, W. de Vries, I. Snellen, A. Koekemoe

Variability and Velocity of Superluminal Sources

We investigate the relation between the Doppler factor determined from variations in total flux at 22 and 37 GHz, and the apparent transverse velocity determined from VLBA observations at 2 cm. The data are consistent with the relativistic beaming theory for compact radio sources, in that the distribution of beta_{app}/delta_{var}, for 30 quasars, is roughly consistent with a Monte Carlo simulation. The intrinsic temperature appears to be ~2x10^{10} K, close to the "equipartition value" calculated by Readhead (1994). We deduce the distribution of Lorentz factors for a group of 48 sources; the values range up to about gamma=40.Comment: To be published in "Radio Astronomy at the Fringe", ASP Conf. Ser. Vol. 300, J. A. Zensus, M. H. Cohen, & E. Ros (eds.), 8 pages, 3 figures, needs rafringe.st

The spatial coherence of noise fields evoked by continuous source distributions

In this work, analytic expressions for the spatial coherence of noise fields are derived in the modal domain with the aim of providing a sparse representation. For this purpose, the sound field in a region of interest is expressed in terms of a given pressure distribution on a virtual surrounding cylindrical or spherical surface. According to the Huygens-Fresnel principle, the sound pressure on this surface is represented by a continuous distribution of elementary line or point sources, where orthogonal basis functions characterize the spatial properties. To describe spatially windowed pressure distributions with arbitrary angular extensions, orthogonal basis functions of limited angular support are proposed. As special cases, circular and spherical pressure distributions with uncorrelated source modes of equal power are investigated. It is shown that these distributions result, respectively, in cylindrically isotropic and spherically isotropic, i.e., diffuse noise fields. The analytic expressions derived in this work allow for a prediction of the spatial coherence between arbitrary positions within the region of interest, such that no microphones need to be placed at the actual points of interest. Simulation results are presented to validate the derived relations.This work was supported by the Australian Research Council (ARC) Discovery Projects funding scheme under Project No. DP140103412

Kinematics of parsec-scale structures in AGN: the 2cm VLBA Survey

We are investigating the kinematics of jets in active galactic nuclei on parsec scales by studying a representative population of sources. This study is being carried out using the Very Long Baseline Array at 15 GHz, with more than 800 images taken since 1994. In this contribution we present an overview of the diversity of kinematics for a complete sample of sources.Comment: Proceedings of the 6th European VLBI Network Symposium, Ros E., Porcas R.W., Lobanov, A.P., & Zensus, J.A. (eds), MPIfR, Bonn, Germany. 2 pages, 3 figures, needs evn2002.cls style fil

Frequency analysis of critical meteorological conditions in a changing climate - Assessing future implications for railway transportation in Austria

Meteorological extreme events have great potential for damaging railway infrastructure and posing risk to the safety of train passengers. In the future, climate change will presumably have serious implications on meteorological hazards in the Alpine region. Hence, attaining insights on future frequencies of meteorological extremes with relevance for the railway operation in Austria is required in the context of a comprehensive and sustainable natural hazard management of the railway operator. In this study, possible impacts of climate change on the frequencies of so-called critical meteorological conditions (CMCs) between the periods 1961-1990 and 2011-2040 are analysed. Thresholds for such CMCs have been defined by the railway operator and used in its weather monitoring and early warning system. First, the seasonal climate change signals for air temperature and precipitation in Austria are described on the basis of an ensemble of high-resolution Regional Climate Model (RCM) simulations for Europe. Subsequently, the RCM-ensemble was used to investigate changes in the frequency of CMCs. Finally, the sensitivity of results is analysed with varying threshold values for the CMCs. Results give robust indications for an all-season air temperature rise, but show no clear tendency in average precipitation. The frequency analyses reveal an increase in intense rainfall events and heat waves, whereas heavy snowfall and cold days are likely to decrease. Furthermore, results indicate that frequencies of CMCs are rather sensitive to changes of thresholds. It thus emphasizes the importance to carefully define, validate and – if needed – to adapt the thresholds that are used in the weather monitoring and warning system of the railway operator. For this, continuous and standardized documentation of damaging events and near-misses is a pre-requisite.JRC.H.7-Climate Risk Managemen

Relativistic Beaming and the Intrinsic Properties of Extragalactic Radio Jets

Relations between the observed quantities for a beamed radio jet, apparent transverse speed and apparent luminosity (beta_app,L), and the intrinsic quantities, Lorentz factor and intrinsic luminosity (gamma,L_o), are investigated. The inversion from measured to intrinsic values is not unique, but approximate limits to gamma and L_o can be found using probability arguments. Roughly half the sources in a flux density--limited, beamed sample have a value of gamma close to the measured beta_app. The methods are applied to observations of 119 AGN jets made with the VLBA at 15 GHz during 1994-2002. The results strongly support the common relativistic beam model for an extragalactic radio jet. The (beta_app,L) data are closely bounded by a theoretical envelope, an aspect curve for gamma=32, L_o= 10^25 W/Hz. This gives limits to the maximum values of gamma and L_o in the sample: gamma_max about 32, and L_o,max ~ 10^26 W/Hz. No sources with both high beta_app and low L are observed. This is not the result of selection effects due to the observing limits, which are flux density S>0.5 Jy, and angular velocity mu<4 mas/yr. Many of the fastest quasars have a pattern Lorentz factor gamma_p close to that of the beam, gamma_b, but some of the slow quasars must have gamma_p<<gamma_b. Three of the 10 galaxies in the sample have a superluminal feature, with speeds up to beta_app about 6. The others are at most mildly relativistic. The galaxies are not off-axis versions of the powerful quasars, but Cygnus A might be an exception.Comment: 12 pages, 9 figures, 1 table, accepted for publication in the Astrophysical Journa

Doppler Boosting, Superluminal Motion, and the Kinematics of AGN Jets

We discuss results from a decade long program to study the fine-scale structure and the kinematics of relativistic AGN jets with the aim of better understanding the acceleration and collimation of the relativistic plasma forming AGN jets. From the observed distribution of brightness temperature, apparent velocity, flux density, time variability, and apparent luminosity, the intrinsic properties of the jets including Lorentz factor, luminosity, orientation, and brightness temperature are discussed. Special attention is given to the jet in M87, which has been studied over a wide range of wavelengths and which, due to its proximity, is observed with excellent spatial resolution. Most radio jets appear quite linear, but we also observe curved non-linear jets and non-radial motions. Sometimes, different features in a given jet appear to follow the same curved path but there is evidence for ballistic trajectories as well. The data are best fit with a distribution of Lorentz factors extending up to gamma ~30 and intrinsic luminosity up to ~10^26 W/Hz. In general, gamma-ray quasars may have somewhat larger Lorentz factors than non gamma-ray quasars. Initially the observed brightness temperature near the base of the jet extend up to ~5x10^13 K which is well in excess of the inverse Compton limit and corresponds to a large excess of particle energy over magnetic energy. However, more typically, the observed brightness temperatures are ~2x10^11 K, i.e., closer to equipartition.Comment: 10 pages, 12 color figures; proceedings of the 5th Stromlo Symposium: Disks, Winds, and Jets - from Planets to Quasars; accepted in Astrophysics & Space Scienc

The LOCATA challenge data corpus for acoustic source localization and tracking

Algorithms for acoustic source localization and tracking are essential for a wide range of applications such as personal assistants, smart homes, tele-conferencing systems, hearing aids, or autonomous systems. Numerous algorithms have been proposed for this purpose which, however, are not evaluated and compared against each other by using a common database so far. The IEEE-AASP Challenge on sound source localization and tracking (LOCATA) provides a novel, comprehensive data corpus for the objective benchmarking of state-of-the-art algorithms on sound source localization and tracking. The data corpus comprises six tasks ranging from the localization of a single static sound source with a static microphone array to the tracking of multiple moving speakers with a moving microphone array. It contains real-world multichannel audio recordings, obtained by hearing aids, microphones integrated in a robot head, a planar and a spherical microphone array in an enclosed acoustic environment as well as positional information about the involved arrays and sound sources represented by moving human talkers or static loudspeakers