Vermiremediation of soils contaminated with mixture of petroleum products using Eisenia fetida

In this paper, vermiremediation, a biological technique was utilized in order to clean-up soil contaminated with gasoline, diesel and spent engine oil using an earthworm - Eisenia fetida. The contaminated soils were analyzed for the total petroleum hydrocarbon (TPH) level every 24 hours over a period of 120 hours using gas chromatography. It was observed that at each sampling time, the soils samples without the earthworm had more quantity of TPH than the corresponding samples with the earthworms. Pentadecane, 2,6,10, trimethyl had 100% reduction after 120 hours followed by octadecane with 67.30 % reduction and tetracosane with 50.28% reduction. In all sampling time, the initial octadecane level was significantly higher than the final octadecane level in soil with E. fetida (P<0.05). Also, the initial hexadecanoic acid methyl ester level was significantly higher than the level of the hydrocarbon in the soils with E. fetida after 96 hour incubation and soil without the earthworm after 72 hours incubation (P<0.05). After the 24 hours incubation the octadecane level in soil with E. fetida was significantly lower than the initial level and the level in soil without the earthworm (P<0.01).The results showed that E. fetida enhances the degradation and reduction of TPH levels in soils and therefore can be used for cleaning up of soils contaminated with mixture of petroleum products. This is useful in reclaiming mechanic workshop soils for agricultural purposes hence increase in food production.Keywords: Vermiremediation, petroleum, contamination, earthworm, Eisenia fetid

Effect of time of application of spent oil on the growth and performance of maize (Zea mays)

The effect of spent oil pollution on the growth and performance of Zea mays at different stages of growth was investigated in this study. It involved addition of different quantities of spent oil to soils where Zea mays plants at different stages of growth were growing on. The plants showed differential response to quantities of spent oil added to the soils and the times of application. Plants exposed to spent oil pollution one week after germination had the highest level of growth inhibition and the highest chlorophyll content. The leaf area development of the plant was inhibited by the exposure of the plant to spent oil pollution as observed seven weeks after germination. The application of spent oil to the soils three and five weeks, respectively after the germination of the seeds of Z. mays had similar effects on dry matter accumulation of the plant. Statistical differences occur on the growth and performance of the plants exposure to spent oil pollution at different stages of growth (p<0.05, p<0.01 and p<0.001). The results from this study showed that generally Z. mays may suffer greater inhibition of growth and performed poorly when it is exposed to spent oil pollution at tender stage of growth.Key words: Spent oil, time application, growth, performance, Zea mays

Serum CA125 and HE4 as Biomarkers for the Detection of Endometrial Cancer and Associated High-Risk Features

Early detection of endometrial cancer improves survival. Non-invasive diagnostic biomarkers would improve triage of symptomatic women for investigations. This study aimed to determine the diagnostic accuracy of serum Cancer Antigen 125 (CA125) and Human Epididymis 4 (HE4) for endometrial cancer and associated high-risk features. Serum samples from women investigated for gynaecological symptoms or diagnosed with endometrial cancer were analysed for CA125 and HE4. Conventional diagnostic metrics were calculated. In total, 755 women were included; 397 had endometrial cancer. Serum CA125 and HE4 were significantly elevated in cases compared with controls (both p < 0.001), and with pathological markers of disease severity (p < 0.05). A combination of CA125 and HE4 detected endometrial cancer with an area under the curve (AUC) of 0.77 (95% CI: 0.74–0.81). In a model with body mass index (BMI) and parity, HE4 predicted endometrial cancer in pre-menopausal women with an AUC of 0.91 [sensitivity = 84.5%, specificity = 80.9% (p < 0.001)]. In women with abnormal ultrasound, HE4 ≥ 77 pmol/L improved specificity compared with imaging alone [68.6% (95% CI: 75.0–83.6) vs. 34.4% (95% CI: 27.1–42.3), respectively], but at a cost to sensitivity. HE4 ≥ 77 pmol/L improved the detection of myometrial invasion ≥50% in women with stage I disease compared with magnetic resonance imaging (MRI) alone [sensitivity = 100% (95% CI: 54.1–100)]. CA125 ≥ 35 U/mL did not add to imaging. HE4 is a good predictor of poor prognostic features which could assist staging investigations

Ambient Air Quality Monitoring in Metropolitan City of Lagos, Nigeria

Twenty one sampling locations were assessed for carbon monoxide (CO), carbondioxide (CO2), oxygen (O2), sulphur dioxide (SO2), nitrogen dioxide (NO2), nitrogen oxide (NO), suspended particulate matter (SPM) and noise level using air pollutants measurement methods approved by ASTM for each specific parameter. All equipments and meters were all properly pre-calibrated before each usage for quality assurance. Findings of the study showed that measured levels of noise (61.4 - 101.4 dBA), NO (0.0 - 3.0 ppm), NO2 (0.0 - 3.0 ppm), CO (1.0 – 42.0 ppm) and SPM (0.14 – 4.82 ppm) in all sampling areas were quite high and above regulatory limits however there was no significant difference except in SPM (at all the sampling points), and noise, NO2 and NO (only in major traffic intersection). Air quality index (AQI) indicates that the ambient air can be described as poor for SPM, varied from good to very poor for CO, while NO and NO2 are very good except at major traffic intersection where they were both poor and very poor (D-E). The results suggest that strict and appropriate vehicle emission management, industrial air pollution control coupled with close burning management of wastes should be considered in the study area to reduce the risks associated with these pollutants.© JASEMKeywords: Air, pollutants, Air quality index, carbon-monoxide, sulphur-dioxid

Potential human health risk assessment of heavy metals intake via consumption of some leafy vegetables obtained from four market in Lagos Metropolis, Nigeria

This work investigated six heavy metals (Pb, Cr, Zn, Cd, Ni and Cu) accumulation in five popular leafy vegetables: Telferia occidentalis (fluted pumpkin), Talinum triangulare (waterleaf), Ocimum gratissimum (scent leaves), Celosia argentea (plumed cockscomb), and Amaranthus viridis (slender amaranth) obtained from 4 popular markets in Lagos metropolis using an atomic absorption spectrophotometer (AAS), and evaluate the human health risks of their consumption. Heavy metals content in vegetables across the markets ranged as follow; Cd (0.05 – 0.20 mg/kg); Pb (0.34 – 5.44 mg/kg), Zn (4.21 – 20.80 mg/kg), Cr (0.25 – 1.51 mg/kg), Ni (0.13 – 2.91 mg/kg) and Cu (2.34 – 14.08 mg/kg). The concentrations of all metals are quite generally lower than the permissible levels by FAO/WHO in vegetables except for Pb. Statistical analysis of levels of the studied metals in A. viridis, T. occidentalis, C. argentea, and O. gratissimum in all the markets sample showed significant differences in levels of Zn, Cr, Cu and Ni (p<0.05). To assess the the health risk of the inhabitants of Lagos and the environs due to heavy metal intake from these vegetables consumption., the daily intake of metals (DIM), health risk index (HRI), and target hazard quotient (THQ) were calculated. The daily intake of metals in vegetables species for Zn (0.51 – 1.46 mg/kg) and Ni (0.05 – 0.22 mg/kg) are significantly lower than the recommended daily intake of metals and the upper tolerable daily intake level (UL). However, DIM of Cd (0.004 – 0.017 mg/kg) and Pb (0.046 – 0.182 mg/kg) exceed the recommended DIM but fall within the upper tolerable daily level. Cr (0.048 – 0.082 mg/kg) is lower than the recommended oral reference dose (RFD) of 1.5 mg/kg (USEPA, 2010). The THQ values range showed that Cd was 0.048 – 0.192, Pb was 0.150 – 0.587, Zn was 0.021 – 0.190, Cr was 0.0001 – 0.001, Ni was 0.050 – 0.120 and Cu was 0.148 – 0.239. This result reflected the risk associated with exposure for the period of life expectancy considered, and the inhabitants are highly exposed to health risks associated to these metals in the order Pb > Cu > Cd > Ni > Zn > Cr. The THQ in all metals is less than 1 in all the vegetables species; therefore, it does not pose serious health risk concern. However, vegetable consumption was just one part of food consumption, the potential health risks for residents might actually be higher than in this study when other routes of heavy metals intake are considered.Keywords: Heavy Metals, leafy vegetable, daily intake of metals, health risk index, target hazard quotient (THQ), zinc, lead, chromium, cadmium, nickel, coppe

Heavy metal analyses and nutritional composition of raw and smoked fishes from Ologe and Lagos Lagoon, Lagos, Nigeria

This study was conducted to evaluate nutritional composition and heavy metals concentration of smoked and raw edible fish species from Ologe lagoon (freshwater) and Lagos lagoon (marine). The fish species from Ologe include: Chrysichthys nigrodigitatus (Catfish), Monodactylus sebae (African moony), Pomadasys jubelini (Buro), Sphyraena barracuda (Great barracuda), and Pseudotolithus elongatus (Croaker), while that of Lagos lagoon include Chrysichthys nigrodigitatus (Catfish), Caranx hippos (Crevalle jack), Liza falcipinnis (Sicklefin mullet), Pomadasys jubelini (Buro), and Monodactylus sebae (African moony). The nutritional composition ranged in the following trend moisture (43.24 - 76.79 %) > carbohydrate (3.16 – 63.45 %) > protein (0.80 - 31.55 %) > fibre (1.07 – 2.8 %) > ash (0.81 – 1.88 %) > fat (0.40 – 1.30 %). In this study, these fishes are generally low in fats (lean fish), and fish smoking tend to reduces their nutritional composition. Heavy metals concentration trend thus Zn (2.4 – 7.75 mg/kg) > Ni (0.80 – 1.925 mg/kg) > Cr (0.20 – 0.550 mg/kg) > Pb (0.025 – 0.50 mg/kg). In all the samples, all the metals of interest were detected except cadmium which was not found in any of the samples analysed. Pb (0.025 - 0.5 mg/kg) values in the fishes were lower than the maximum standard level reported to be 0.5 mg/kg dry weight by FAO except in the raw Pomadasys jubelini where the value is 0.5mg/kg. The fresh water fishes showed higher concentration of heavy metals than marine water fishes but not statistically significant (p>0.05). Although, smoked fishes showed higher concentration of heavy metals than the raw fishes, it was only significant for zinc (p<0.05). In view of this study and the importance of fish in human diet, it is recommended that biological monitoring of fishes meant for consumption from these two water bodies be carried out regularly to ensure human safety.Keywords: Nutritional composition, heavy metals, fresh water fishes, marine water fishes, lagoon

Bacteria Associated with Domestic Solid Waste Dump in Egbu, Owerri North Local Government Area in Imo State, Nigeria.

The prevalence of bacteria in solid waste dump from domestic waste in Owerri was studied. Six samples were collected from Egbu community in Owerri North and examined bacteriologically using Nutrient agar and macconkey agar and were serially diluted, cultured, identified and characterized. Five genera of bacteria were isolated. They were Staphylococcus aureus, Klebsiella sp, Escherichia coli, lactobacillus sp and Corynebacterium sp and from the findings,Klebsiella exhibited the highest  number of bacterial activity (66.6%) while Corynebacterium and Iactobacillus exhibited the lowest number of bacterial activity (16.67%). Futhermore, location D had the highest total heterotropic bacterial count of 5.75x 108 and the least was location E with 6.25x107. The presence of Escherichia coli, Staphylococcus aureus, Cornebacteria and Klebsiella in the waste dump site is an indication that the contamination is of human and plant orign or their activities. Keywords: Nutrient agar ,macconkey agar , Staphylococcus aurous, Klebsiellasp, Escherichia coli,  Lactobacillus sp and Corynebacterium sp.

Effect of Plant Spacing on the Growth and Yield of Forage Plant, Maize, Intercropped with Cowpea (\u3cem\u3eVigna unguiculata\u3c/em\u3e sub spp. \u3cem\u3esesquipedalis\u3c/em\u3e)

Feed constraint is the most important impediment to improved livestock production in the Sub-Saharan African (SSA) countries (Agyemang 2003), as a result of seasonal shortages in the quantity and quality of forage from natural pastures that supply most of the feed for animals due to the prolonged annual dry season. Appropriate technologies to improve the performance of the local animal breeds and feed resources under the traditional system are generally lacking. Maize forms a major part of the cereal crops consumed by man and the residues serve as a source of fodder for livestock (Asawalam and Adesiyan 2001). Demand for maize has led farmers to reduce planting spacing. When crops are grown sole, spacing and planting pattern differ from when intercropped. Maize is known to be a very heavy soil nutrient utiliser and usually produces low yield in situations of low soil fertility and poor agronomic practices. Intercropping maize with legumes has shown prospects for improving maize crop yield and this would result in a reduction in the use of high cost synthetic fertilizers. The cowpea is an example of such legume and little work has been done on plant spacing for this crop in an intercropping system with maize. Plant spacing is an important agronomic attribute as it has effects on light interception which is the energy manufacturing medium of green parts of the plant (Ibeawuchi, et al. 2008)

DEveloping Tests for Endometrial Cancer deTection (DETECT): protocol for a diagnostic accuracy study of urine and vaginal samples for the detection of endometrial cancer by cytology in women with postmenopausal bleeding.

From Europe PMC via Jisc Publications RouterHistory: ppub 2021-07-01, epub 2021-07-28Publication status: PublishedFunder: Wellcome TrustFunder: Department of Health; Grant(s): NIHR300650Funder: Cancer Research UK; Grant(s): C147/A25254IntroductionPostmenopausal bleeding (PMB), the red flag symptom for endometrial cancer, triggers urgent investigation by transvaginal ultrasound scan, hysteroscopy and/or endometrial biopsy. These investigations are costly, invasive and often painful or distressing for women. In a pilot study, we found that voided urine and non-invasive vaginal samples from women with endometrial cancer contain malignant cells that can be identified by cytology. The aim of the DEveloping Tests for Endometrial Cancer deTection (DETECT) Study is to determine the diagnostic test accuracy of urine and vaginal cytology for endometrial cancer detection in women with PMB.Methods and analysisThis is a multicentre diagnostic accuracy study of women referred to secondary care with PMB. Eligible women will be asked to provide a self-collected voided urine sample and a vaginal sample collected with a Delphi screener before routine clinical procedures. Pairs of specialist cytologists, blinded to participant cancer status, will assess and classify samples independently, with differences settled by consensus review or involving a third cytologist. Results will be compared with clinical outcomes from standard diagnostic tests. A sample size of 2000 women will have 80% power to establish a sensitivity of vaginal samples for endometrial cancer detection by cytology of ≥85%±7%, assuming 5% endometrial cancer prevalence. The primary objective is to determine the diagnostic accuracy of urogenital samples for endometrial cancer detection by cytology. Secondary objectives include the acceptability of urine and vaginal sampling to women.Ethics and disseminationThis study has been approved by the North West-Greater Manchester West Research Ethics Committee (16/NW/0660) and the Health Research Authority. Results will be disseminated through publication in peer-reviewed scientific journals, presentation at conferences and via charity websites.Trial registration numberISRCTN58863784