1,984 research outputs found
The People vs. The Florida Manatee: A Review of the Laws Protecting Florida\u27s Endangered Marine Mammal and Need for Application
Florida manatees (Trichechus manatus latirostris) are a tropical species endemic at their northernmost habitat range within the southeastern United States. Manatees face a thermoregulatory requirement during winter months and follow a seasonal migration to warm water sources. To avoid cold stress syndrome (CSS), manatees utilize the warmth from artificial sources such as power plant discharge canals or natural sources such as artesian springs. Already endangered, this species nonetheless continually faces an ever-growing threat from human impact within these important locations. This paper reviews the past and present laws protecting manatees in Florida, chronicles the impacts manatees are facing presently and in the future, and details the increasing need for the application of protection from a management standpoint. With the correct management plan in place, manatees and humans can cooperatively coexist together in a shared environment
Exposure to the leaf litter microbiome of healthy adults protects seedlings from pathogen damage
It is increasingly recognized that microbiota affect host health and physiology. However, it is unclear what factors shape microbiome community assembly in nature, and how microbiome assembly can be manipulated to improve host health. All plant leaves host foliar endophytic fungi, which make up a diverse, environmentally acquired fungal microbiota. Here, we experimentally manipulated assembly of the cacao tree (Theobroma cacao) fungal microbiome in nature and tested the effect of assembly outcome on host health. Using next-generation sequencing, as well as culture-based methods coupled with Sanger sequencing, we found that manipulating leaf litter exposure and location within the forest canopy significantly altered microbiome composition in cacao. Exposing cacao seedlings to leaf litter from healthy conspecific adults enriched the seedling microbiome with Colletotrichum tropicale, a fungal endophyte known to enhance pathogen resistance of cacao seedlings by upregulating host defensive pathways. As a result, seedlings exposed to healthy conspecific litter experienced reduced pathogen damage. Our results link processes that affect the assembly and composition of microbiome communities to their functional consequences for host success, and have broad implications for understanding plantâmicrobe interactions. Deliberate manipulation of the plantâ fungal microbiome also has potentially important applications for cacao production and other agricultural systems in generalIt is increasingly recognized that microbiota affect host health and physiology. However, it is unclear what factors shape microbiome community assembly in nature, and how microbiome assembly can be manipulated to improve host health. All plant leaves host foliar endophytic fungi, which make up a diverse, environmentally acquired fungal microbiota. Here, we experimentally manipulated assembly of the cacao tree (Theobroma cacao) fungal microbiome in nature and tested the effect of assembly outcome on host health. Using next-generation sequencing, as well as culture-based methods coupled with Sanger sequencing, we found that manipulating leaf litter exposure and location within the forest canopy significantly altered microbiome composition in cacao. Exposing cacao seedlings to leaf litter from healthy conspecific adults enriched the seedling microbiome with Colletotrichum tropicale, a fungal endophyte known to enhance pathogen resistance of cacao seedlings by upregulating host defensive pathways. As a result, seedlings exposed to healthy conspecific litter experienced reduced pathogen damage. Our results link processes that affect the assembly and composition of microbiome communities to their functional consequences for host success, and have broad implications for understanding plantâmicrobe interactions. Deliberate manipulation of the plantâ fungal microbiome also has potentially important applications for cacao production and other agricultural systems in genera
Visual census, photographic records and the trial of a video network provide first evidence of the elusive Sicyopterus cynocephalus in Australia
Opportunistic encounters with an elusive large-bodied sicydiine goby in a single plunge pool led us to photograph and deploy three video cameras to detect individuals in that pool. Subsequently, a catchment-wide search indicated that the species, eventually identified as Sicyopterus cynocephalus, was confined to the single pool where it was originally detected. A network of ten video cameras was then deployed to estimate the number of individuals of that species and of a congener, S. lagocephalus, by non-destructive means. This study provides the first record of S. cynocephalus in Australia, and showcases the synergy of active snorkel searches and a remote camera network in counting individuals of two sympatric species of Sicyopterus
A finite element model for predicting impact-induced damage to a skin simulant
A finite element model was developed for assessing the efficacy of rugby body padding in reducing the risk of sustaining cuts and abrasions. The model was developed to predict the onset of damage to a soft tissue simulant from concentrated impact loading (i.e., stud impact) and compared against a corresponding experiment. The damage modelling techniques involved defining an element deletion criterion, whereby those on the surface of the surrogate were deleted if their maximum principal stress reached a predefined value. Candidate maximum principal stress values for element deletion criteria were identified independently from puncture test simulations on the soft tissue simulant. Experimental impacts with a stud were carried out at three energies (2, 4 and 6 J), at three angular orientations (0°, 15° and 30°) and compared to corresponding simulations. Suitable maximum principal stress values for element deletion criteria settings were first identified for the 4 J impact, selecting the candidates that best matched the experimental results. The same element deletion settings were then applied in simulations at 2 and 6 J and the validity of the model was further assessed (differenceâ<â15% for the force at tear andâ<â30% for time to tear). The damage modelling techniques presented here could be applied to other skin simulants to assess the onset of skin injuries and the ability of padding to prevent them
Establishment and persistence of legumes on sites varying in aspect, landscape position and soil type
Most Iowa pastures display only a small variety of plant species, resulting in large seasonal and annual variations in pasture productivity. This project evaluated the causes for these variations in cool-season grass pastures and considered ways to improve diversity of legume species used for grazing
Prospective, randomized, multi-institutional clinical trial of a silver alginate dressing to reduce lower extremity vascular surgery wound complications
ObjectiveWound complications negatively affect outcomes of lower extremity arterial reconstruction. By way of an investigator initiated clinical trial, we tested the hypothesis that a silver-eluting alginate topical surgical dressing would lower wound complication rates in patients undergoing open arterial procedures in the lower extremity.MethodsThe study block-randomized 500 patients at three institutions to standard gauze or silver alginate dressings placed over incisions after leg arterial surgery. This original operating room dressing remained until gross soiling, clinical need to remove, or postoperative day 3, whichever was first. Subsequent care was at the provider's discretion. The primary end point was 30-day wound complication incidence generally based on National Surgical Quality Improvement Program guidelines. Demographic, clinical, quality of life, and economic end points were also collected. Wound closure was at the surgeon's discretion.ResultsParticipants (72% male) were 84% white, 45% were diabetic, 41% had critical limb ischemia, and 32% had claudication (with aneurysm, bypass revision, other). The overall 30-day wound complication incidence was 30%, with superficial surgical site infection as the most common. In intent-to-treat analysis, silver alginate had no effect on wound complications. Multivariable analysis showed that Coumadin (Bristol-Myers Squibb, Princeton, NJ; odds ratio [OR], 1.72; 95% confidence interval [CI], 1.03-2.87; PÂ = .03), higher body mass index (OR, 1.05; 95% CI, 1.01-1.09; PÂ = .01), and the use of no conduit/material (OR, 0.12; 95% CI, 0.82-3.59; PÂ < .001) were independently associated with wound complications.ConclusionsThe incidence of wound complications remains high in contemporary open lower extremity arterial surgery. Under the study conditions, a silver-eluting alginate dressing showed no effect on the incidence of wound complications
Resorption controls bone anabolism driven by PTH receptor signaling in osteocytes
The contribution of remodeling-based bone formation coupled to osteoclast activity versus modeling-based bone formation that occurs independently of resorption, to the anabolic effect of PTH remains unclear. We addressed this question using transgenic mice with activated PTH receptor signaling in osteocytes that exhibit increased bone mass and remodeling, recognized skeletal effects of PTH elevation. Direct inhibition of bone formation was accomplished genetically by overexpressing the Wnt antagonist Sost/sclerostin; and resorption-dependent bone formation was inhibited pharmacologically with the bisphosphonate alendronate. We found that bone formation induced by osteocytic PTH receptor signaling on the periosteal surface depends on Wnt signaling but not on resorption. In contrast, bone formation on the endocortical surface results from a combination of Wnt-driven increased osteoblast number and resorption-dependent osteoblast activity. Moreover, elevated osteoclasts and intracortical/calvarial porosity is exacerbated by overexpressing Sost and reversed by blocking resorption. Furthermore, increased cancellous bone is abolished by Wnt inhibition but further increased by blocking resorption. Thus, resorption induced by PTH receptor signaling in osteocytes is critical for full anabolism in cortical bone, but tempers bone gain in cancellous bone. Dissecting underlying mechanisms of PTH receptor signaling would allow targeting actions in different bone compartments, enhancing the therapeutic potential of the pathway
Morally Respectful Listening and its Epistemic Consequences
What does it mean to listen to someone respectfully, that is, insofar as they are due recognition respect? This paper addresses that question and gives the following answer: it is to listen in such a way that you are open to being surprised. A specific interpretation of this openness to surprise is then defended
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