CCL21/CCR7 enhances the proliferation, migration, and invasion of human bladder cancer T24 cells

Objective To investigate the effects of CCL21/CCR7 on the proliferation, migration, and invasion of T24 cells and the possible associated mechanisms: expression of MMP-2 and MMP-9, and regulation of BCL-2 and BAX proteins. Methods T24 cells received corresponding treatments including vehicle control, antibody (20ng/mL CCR7 antibody and 50 ng/ml CCL21), and 50, 100. and 200 ng/ml CCL21. Proliferation was evaluated by MTT assay; cell migration and invasion were assayed using a transwell chamber. Cell apoptosis was induced by Adriamycin (ADM). The rate of cell apoptosis was examined by flow cytometry using annexin V-FITC/PI staining. Western-blot was used to analyze MMP-2 and MMP-9 and BCL-2 and BAX proteins. Results CCL21 promoted T24 cell proliferation in concentration-dependent manner with that 200 ng/mL induced the largest amount of proliferation. Significant differences of cell migration were found between CCL21treatment groups and the control group in both the migration and invasion studies (P \u3c 0.001 for all). The expressions of MMP-2 and MMP-9 proteins were significantly increased after CCL21 treatment (p \u3c 0.05 for all). Protein expression of Bcl-21 follows an ascending trend while the expression of Bax follows a descending trend as the concentration of CCL21 increases. No difference was found between the control group and antibody group for all assessments. Conclusion CCL21/CCR7 promoted T24 cell proliferation and enhanced its migration and invasion via the increased expression of MMP-2 and MMP-9. CCL21/CCR7 had antiapoptotic activities on T24 cells via regulation of Bcl-2 and Bax proteins. CCL21/CCR7 may promote bladder cancer development and metastasis

An indoor positioning technology based on GA-BP Neural Network

Conference Name:6th International Conference on Computer Science and Education, ICCSE 2011. Conference Address: Singapore, Singapore. Time:August 3, 2011 - August 5, 2011.With the development of wireless positioning technologies, indoor positioning technologies are getting more and more noticeable. RFID positioning technology reaches the goal of recognizing and positioning by using radio frequency to transmit data through non-contact two-way communication. This technology has the advantages of long transmission effective range, low-cost, non-contact, non-line-of-sight and so on. Using the technology, a GA-BP Neural Network based indoor positioning algorithm is proposed in this thesis, series of tests to it in practical setting are also carried out. The result shows that the algorithm can solve the problems caused by the complication and variation of indoor environment at a certain degree. ? 2011 IEEE

Adaptive control of biped robots based on Gennet

Conference Name:2012 7th International Conference on Computer Science and Education, ICCSE 2012. Conference Address: Melbourne, VIC, Australia. Time:July 14, 2012 - July 17, 2012.University of MelbourneThe dynamic walking stability of robots is a key to biped robots study. Due to the uncertainty of outside environment, adaptability has become a priority in the research of dynamic walking of robots. In order to realize the dynamic walking of robots, a Genetic Neural Network is used to develop an Adaptive Control Neural Network. A better walking result is realized by the Neural Network trained by abnormal walking samples and self-adaptive fitness formulation. 漏 2012 IEEE

A brief discussion on the build of program architecture in RoboCup 3D simulation environment

Conference Name:2012 7th International Conference on Computer Science and Education, ICCSE 2012. Conference Address: Melbourne, VIC, Australia. Time:July 14, 2012 - July 17, 2012.University of MelbourneAccompanied with a series changes in RoboCup 3D simulation condition, the competition environment has become much more sophisticated than before, which contributes to a complicated program structure. In this paper, an architecture which formed by four layers is introduced in the build of RoboCup 3D Client program considering the configuration of RoboCup 3D system and the architecture of agent. The features and relationship among those layers are expounded and demonstrated in graph. 漏 2012 IEEE

3D human body inpainting using intrinsic statistical shape models

This paper presents an algorithm for inpainting 3D human body geometry. We built a Statistical Shape Model (SSM) based on the intrinsic Laplacian Coordinates (LC) to tackle the pose variation of human bodies, which is a common challenging issue in 3D human body modeling and shape analysis. We evaluate our inpainting algorithm using synthesized damages on complete human body scans, and with the groundtruth we measure the normalized reconstruction error quantitatively. Our experiments demonstrate the effectiveness of this LC-based SSM in human body inpainting

A Microservice-Based Big Data Analysis Platform for Online Educational Applications

The booming development of data science and big data technology stacks has inspired continuous iterative updates of data science research or working methods. At present, the granularity of the labor division between data science and big data is more refined. Traditional work methods, from work infrastructure environment construction to data modelling and analysis of working methods, will greatly delay work and research efficiency. In this paper, we focus on the purpose of the current friendly collaboration of the data science team to build data science and big data analysis application platform based on microservices architecture for education or nonprofessional research field. In the environment based on microservices that facilitates updating the components of each component, the platform has a personal code experiment environment that integrates JupyterHub based on Spark and HDFS for multiuser use and a visualized modelling tools which follow the modular design of data science engineering based on Greenplum in-database analysis. The entire web service system is developed based on spring boot

The complete mitochondrial genome of Pseudomugil furcatus (Atheriniformes: Pseudomugil) and phylogenetic studies of Atheriniformes

Complete mitochondrial genome of the atheriniform fish Pseudomugil furcatus was characterized in this study. The whole mitogenome was 16,753 bp in size and consisted of 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs genes, a control region, and origin of light-strand replication. The proportion of coding sequences with a total length of 11,499 bp was 68.64%, which encoded 3869 amino acids. The base composition of the genome was 27.00% for A, 27.31% for C, 15.56% for G, and 30.13% for T. All PCGs started with ATG, ATP8, ND3, ND5, and ND6 ended with TAG, whereas CO2, ATP6, ND4 ended with a single T, the other PCGs commonly ended with TAA. The length of 12S ribosomal RNA was 948 bp, while that of 16S ribosomal RNA was 1744 bp. The control region (D-loop) ranging from 15,774 to 16,753 bp was 980 bp in size. The complete mitochondrial genome sequence provided here could be a useful information for the management of P. furcatus

Phylogenetic analysis of the complete mitochondrial genome of Orestias ascotanensis (Cyprinodontiformes, Cyprinodontidae)

The complete mitochondrial genome of this species was first determined in this study, which is 16,617 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, a putative control region, and 1 origin of replication on the light-strand. The overall base composition includes C(27.11%), A(26.68%), T(29.15%), G(17.04%) and three degenerate bases are R, R and S. Moreover, the 13 PCGs encode 3800 amino acids in total, 12 of which use the initiation codon ATG except COI that uses GTG. Most of them have TAA as the stop codon, whereas ND5 ends with AGA, four protein-coding genes (ND1, ND2, ND3 and Cytb) ended with TAG, and two protein-coding genes (COII and ND4) ended with the incomplete stop codon represented as a single T. The phylogenetic tree based on the Neighbor Joining method was constructed to provide relationship within Cyprinodontidae, which could be a useful basis for management of this species

The complete mitochondrial genome of Hemigrammus bleheri (Characiformes: Hemigrammus) and phylogenetic studies of Characiformes

Complete mitochondrial genome of the characiform fish Hemigrammus bleheri was characterized in the present study. The whole mitogenome was 17,021 bp in size and consisted of 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs genes, a control region, and origin of light-strand replication. The proportion of coding sequences with a total length of 11,415 bp is 67.06%, which encodes 3805 amino acids. Similar to other Hemigrammus species, the base composition of H. bleheri was 29.30% for A, 25.26% for C, 16.36% for G, and 29.08% for T. All PCGs started with Met. ND1, ND3, ND4L, ND6, and CytB ended with TAA as the stop codon. ND2, ATP8, and ND5 ended with TAG as a stop codon, CO2, ATP6, CO3, and ND4 ended simply by T, and CO1 ended by a single AGG. The lengths of 12S ribosomal RNA and 16S ribosomal RNA were 924 bp and 1681 bp, respectively. The length of control region (D-loop) was 1308 bp, ranging from 15,714 to 17,021 bp. The complete mitochondrial genome sequence provided here would be helpful in further understanding the evolution of characiformes and conservation genetics of H. bleheri

Effects of CCL21 with or without CCR7 antibody on the expressions of Bcl-2 and Bax.

<p>T24 cells were treated with vehicle control, CCR7 antibody 20 ng/ml plus CCL21 50 ng/ml, CCL21 50 ng/ml, CCL21 100 ng/ml, and CCL21 200 ng/ml. The values (OD) present Mean ± SD from three independent experiments.</p