Implications of territory size for the measurement and prediction of salmonid abundance in streams

Information about territory size is useful for both the measurement and prediction of salmonid abundance. Percent habitat saturation (PHS), the percentage of the stream area occupied by the territories of salmonid fishes, is a better measure of abundance than population density because the former integrates the effects of (a) several age-classes or species in a stream, and (b) variation in growth rate or sampling date. "Effective density" or "effective PHS," calculated by weighting crude density (no.·m-2) or PHS by the number of organisms in the sampling unit, more accurately reflects density from the organism's point of view than does crude density or PHS. Effective density and PHS of Atlantic salmon (Salmo salar) in Catamaran Brook, New Brunswick, increased by 0.4 fish per m2 and 4%, respectively, for each order of magnitude decrease in the area of the sampling unit. Literature data suggested that territory size is inversely related to food abundance and can be used to predict changes in salmonid abundance that accompany changes in food abundance. The allometry of territory size was a better predictor of the decline in density of a cohort of Atlantic salmon in Catamaran Brook than the allometry of metabolic requirements

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

Multiple novel prostate cancer susceptibility signals identified by fine-mapping of known risk loci among Europeans

Genome-wide association studies (GWAS) have identified numerous common prostate cancer (PrCa) susceptibility loci. We have fine-mapped 64 GWAS regions known at the conclusion of the iCOGS study using large-scale genotyping and imputation in 25 723 PrCa cases and 26 274 controls of European ancestry. We detected evidence for multiple independent signals at 16 regions, 12 of which contained additional newly identified significant associations. A single signal comprising a spectrum of correlated variation was observed at 39 regions; 35 of which are now described by a novel more significantly associated lead SNP, while the originally reported variant remained as the lead SNP only in 4 regions. We also confirmed two association signals in Europeans that had been previously reported only in East-Asian GWAS. Based on statistical evidence and linkage disequilibrium (LD) structure, we have curated and narrowed down the list of the most likely candidate causal variants for each region. Functional annotation using data from ENCODE filtered for PrCa cell lines and eQTL analysis demonstrated significant enrichment for overlap with bio-features within this set. By incorporating the novel risk variants identified here alongside the refined data for existing association signals, we estimate that these loci now explain ∼38.9% of the familial relative risk of PrCa, an 8.9% improvement over the previously reported GWAS tag SNPs. This suggests that a significant fraction of the heritability of PrCa may have been hidden during the discovery phase of GWAS, in particular due to the presence of multiple independent signals within the same regio

Prey size of salmonid fishes in streams, lakes, and oceans

The growth rate of salmonid fishes is typically fastest in oceans, intermediate in lakes, and slowest in streams. We compiled literature data to test whether differences in the size of prey eaten by salmonids in the three habitats could account for these differences in growth rate (i.e., the prey-size hypothesis). In all three habitats, salmonid fishes exhibited ontogenetic niche shifts from feeding primarily on invertebrates when small to feeding on fishes when large. Contrary to the prey-size hypothesis, invertebrates eaten in streams were larger than those eaten in lakes or oceans, whereas fish eaten in oceans were smaller than those eaten in streams or lakes. Consistent with the prey-size hypothesis, salmonids began eating fish at a smaller size in oceans (8 cm) than in lakes (15 cm) or streams (27 cm). However, the size at which salmonids became predominantly piscivorous (31 cm) did not differ significantly between habitats. We suggest that the difference in growth potential between the three habitats is partly related to the size at which fish first enter the diet

Allometry of diet selectivity in juvenile Atlantic salmon (Salmo salar)

Juvenile salmonids in streams typically feed on larger invertebrates than the average size available in the drift. Our objective was to describe the allometry of this size-selective foraging in juvenile Atlantic salmon, Salmo salar of Catamaran Brook, New Brunswick. We compared paired samples of the stomach contents of 46 salmon (age 0 + to 2 + ; fork length 2.9-14.5 cm) with drift samples collected from their feeding territories. Juvenile salmon fed opportunistically on all major types of invertebrates in the drift, except for water mites (Hydracarina). However, newly emerged salmon fed on smaller prey than the average available in the drift, primarily chironomid larvae, whereas salmon larger than 4.6 cm fed on larger prey than average, primarily dipteran adults and pupae. Larger salmon ate larger prey. Minimum prey length in stomachs was well predicted by gill raker spacing, but mean prey width was only one third of the optimal size and maximum prey width was much less than mouth width. The allometry of prey size appeared to be related primarily to an increase in size-selective foraging with increasing body size, rather than to morphological constraints. Juvenile Atlantic salmon in our study ate smaller prey than similar-sized salmonids in other studies. </jats:p

The effect of visual isolation on territory size and population density of juvenile rainbow trout (Oncorhynchus mykiss)

Visibility is thought to affect the territory size of visually oriented animals but there have been few experimental tests of the hypothesis. We re-examined the relationship between visibility and territory size in juvenile salmonids to test the hypothesis that increasing habitat heterogeneity results in a reduction in territory size and consequently in higher population densities. Equal densities of young-of-the-year rainbow trout (Oncorhynchus mykiss) were stocked in two experimental treatments with low visibility and a control treatment with high visibility. Visibility was decreased by placing large stones or plywood dividers onto the substrate of experimental stream channels. As predicted, the size of individual territories decreased with decreasing visibility of the habitat. However, the treatments did not differ significantly in population density or growth rate of the fish. While this study confirms the inverse relationship between habitat visibility and territory size, the decrease in territory size did not produce an increase in population density of juvenile salmonids

Conservation of Native Pacific Trout Diversity in Western North America

<p>Pacific trout <i>Oncorhynchus</i> spp. in western North America are strongly valued in ecological, socioeconomic, and cultural views, and have been the subject of substantial research and conservation efforts. Despite this, the understanding of their evolutionary histories, overall diversity, and challenges to their conservation is incomplete. We review the state of knowledge on these important issues, focusing on Pacific trout in the genus <i>Oncorhynchus</i>. Although most research on salmonid fishes emphasizes Pacific salmon, we focus on Pacific trout because they share a common evolutionary history, and many taxa in western North America have not been formally described, particularly in the southern extent of their ranges. Research in recent decades has led to the revision of many hypotheses concerning the origin and diversification of Pacific trout throughout their range. Although there has been significant success at addressing past threats to Pacific trout, contemporary and future threats represented by nonnative species, land and water use activities, and climate change pose challenges and uncertainties. Ultimately, conservation of Pacific trout depends on how well these issues are understood and addressed, and on solutions that allow these species to coexist with a growing scope of human influences.</p

Beliefs in Conspiracy Theories Among African Americans: A Comparison of Elites and Masses

Several studies have reported a widespread belief in conspiracy theories among African Americans. Such theories have been shown to have possible deleterious effects, especially when they deal with HIV/AIDS. It has been conjectured that African-American elites could play a role in dispelling these beliefs, unless, of course, they believe in these theories themselves. To examine this possibility the present study examines the conspiratorial beliefs of African-American locally elected officials in Louisiana and compares them with a previous study of African-American churchgoers in the same state. Copyright (c) 2005 by the Southwestern Social Science Association.