4-Year COBE DMR Cosmic Microwave Background Observations: Maps and Basic Results

The cosmic microwave background radiation provides unique constraints on cosmological models. In this Letter we present a summary of the spatial properties of the cosmic microwave background radiation based on the full 4 years of COBE DMR observations, as detailed in a set of companion Letters. The anisotropy is consistent with a scale-invariant power law model and Gaussian statistics. With full use of the multi-frequency 4-year DMR data, including our estimate of the effects of Galactic emission, we find a power-law spectral index of $n=1.2\pm 0.3$ and a quadrupole normalization $Q_{rms-PS}=15.3^{+3.8}_{-2.8}$ $\mu$K. For $n=1$ the best-fit normalization is $Q_{rms-PS}\vert_{n=1}=18\pm 1.6$ $\mu$K. These values are consistent with both our previous 1-year and 2-year results. The results include use of the $\ell=2$ quadrupole term; exclusion of this term gives consistent results, but with larger uncertainties. The 4-year sky maps, presented in this Letter, portray an accurate overall visual impression of the anisotropy since the signal-to-noise ratio is ~2 per 10 degree sky map patch. The improved signal-to-noise ratio of the 4-year maps also allows for improvements in Galactic modeling and limits on non-Gaussian statistics.Comment: 11 pages plus 2 PostScript figures. Figures 2 and 4 are not included, but are available upon request to [email protected]. Submitted to The Astrophysical Journal (Letters

Dipole Anisotropy in the COBE DMR First-Year Sky Maps

We present a determination of the cosmic microwave background dipole amplitude and direction from the COBE Differential Microwave Radiometers (DMR) first year of data. Data from the six DMR channels are consistent with a Doppler-shifted Planck function of dipole amplitude Delta T = 3.365 +/-0.027 mK toward direction (l,b) = (264.4 +/- 0.3 deg, 48.4 +/- 0.5 deg). The implied velocity of the Local Group with respect to the CMB rest frame is 627 +/- 22 km/s toward (l,b) = (276 +/- 3 deg, 30 +/- 3 deg). DMR has also mapped the dipole anisotropy resulting from the Earth's orbital motion about the Solar system barycenter, yielding a measurement of the monopole CMB temperature at 31.5, 53, and 90 GHz, to be 2.75 +/- 0.05 K.Comment: Post Script (4 figures) Ap J 419, 1-6 (1993

FOUR-YEAR COBE 1 DMR COSMIC MICROWAVE BACKGROUND OBSERVATIONS: MAPS AND BASIC RESULTS

ABSTRACT In this Letter we present a summary of the spatial properties of the cosmic microwave background radiation based on the full 4 yr of COBE Differential Microwave Radiometer (DMR) observations, with additional details in a set of companion Letters. The anisotropy is consistent with a scale-invariant power-law model and Gaussian statistics. With full use of the multifrequency 4 yr DMR data, including our estimate of the effects of Galactic emission, we find a power-law spectral index of n ϭ 1.2 H 0.3 and a quadrupole normalization Q rmsϪPS ϭ 15.3 Ϫ2.8 ϩ3.8 K. For n ϭ 1 the best-fit normalization is Q rmsϪPS͉n ϭ 1 ϭ 18 H 1.6 K. These values are consistent with both our previous 1 yr and 2 yr results. The results include use of the ᐉ ϭ 2 quadrupole term; exclusion of this term gives consistent results, but with larger uncertainties. The final DMR 4 yr sky maps, presented in this Letter, portray an accurate overall visual impression of the anisotropy since the signal-to-noise ratio is 12 per 10Њ sky map patch. The improved signal-to-noise ratio of the 4 yr maps also allows for improvements in Galactic modeling and limits on non-Gaussian statistics

Attachment of Salmonella strains to a plant cell wall model is modulated by surface characteristics and not by specific carbohydrate interactions

Background: Processing of fresh produce exposes cut surfaces of plant cell walls that then become vulnerable to human foodborne pathogen attachment and contamination, particularly by Salmonella enterica. Plant cell walls are mainly composed of the polysaccharides cellulose, pectin and hemicelluloses (predominantly xyloglucan). Our previous work used bacterial cellulose-based plant cell wall models to study the interaction between Salmonella and the various plant cell wall components. We demonstrated that Salmonella attachment was favoured in the presence of pectin while xyloglucan had no effect on its attachment. Xyloglucan significantly increased the attachment of Salmonella cells to the plant cell wall model only when it was in association with pectin. In this study, we investigate whether the plant cell wall polysaccharides mediate Salmonella attachment to the bacterial cellulose-based plant cell wall models through specific carbohydrate interactions or through the effects of carbohydrates on the physical characteristics of the attachment surface. Results: We found that none of the monosaccharides that make up the plant cell wall polysaccharides specifically inhibit Salmonella attachment to the bacterial cellulose-based plant cell wall models. Confocal laser scanning microscopy showed that Salmonella cells can penetrate and attach within the tightly arranged bacterial cellulose network. Analysis of images obtained from atomic force microscopy revealed that the bacterial cellulose-pectin-xyloglucan composite with 0.3 % (w/v) xyloglucan, previously shown to have the highest number of Salmonella cells attached to it, had significantly thicker cellulose fibrils compared to other composites. Scanning electron microscopy images also showed that the bacterial cellulose and bacterial cellulose-xyloglucan composites were more porous when compared to the other composites containing pectin. Conclusions: Our study found that the attachment of Salmonella cells to cut plant cell walls was not mediated by specific carbohydrate interactions. This suggests that the attachment of Salmonella strains to the plant cell wall models were more dependent on the structural characteristics of the attachment surface. Pectin reduces the porosity and space between cellulose fibrils, which then forms a matrix that is able to retain Salmonella cells within the bacterial cellulose network. When present with pectin, xyloglucan provides a greater surface for Salmonella cells to attach through the thickening of cellulose fibrils

Biochemical characterization and low-resolution SAXS shape of a novel GH11 exo-1,4-β-xylanase identified in a microbial consortium

Biotechnologies that aim to produce renewable fuels, chemicals, and bioproducts from residual ligno(hemi)cellulosic biomass mostly rely on enzymatic depolymerization of plant cell walls (PCW). This process requires an arsenal of diverse enzymes, including xylanases, which synergistically act on the hemicellulose, reducing the long and complex xylan chains to oligomers and simple sugars. Thus, xylanases play a crucial role in PCW depolymerization. Until recently, the largest xylanase family, glycoside hydrolase family 11 (GH11) has been exclusively represented by endo-catalytic β-1,4- and β-1,3-xylanases. Analysis of a metatranscriptome library from a microbial lignocellulose community resulted in the identification of an unusual exo-acting GH11 β-1,4-xylanase (MetXyn11). Detailed characterization has been performed on recombinant MetXyn11 including determination of its low-resolution small angle Xray scattering (SAXS) molecular envelope in solution. Our results reveal that MetXyn11 is a monomeric globular enzyme that liberates xylobiose from heteroxylans as the only product. MetXyn11 has an optimal activity in a pH range from 6 to 9 and an optimal temperature of 50 oC. The enzyme maintained above 65% of its original activity in the pH range 5 to 6 after being incubated for 72 h at 50 oC. Addition of the enzyme to a commercial enzymatic cocktail (CelicCtec3) promoted a significant increase of enzymatic hydrolysis yields of hydrothermally pretreated sugarcane bagasse (16% after 24 h of hydrolysis)

Inducible expression of Pisum sativum xyloglucan fucosyltransferase in the pea root cap meristem, and effects of antisense mRNA expression on root cap cell wall structural integrity

Mitosis and cell wall synthesis in the legume root cap meristem can be induced and synchronized by the nondestructive removal of border cells from the cap periphery. Newly synthesized cells can be examined microscopically as they differentiate progressively during cap development, and ultimately detach as a new population of border cells. This system was used to demonstrate that Pisum sativum L. fucosyl transferase (PsFut1) mRNA expression is strongly expressed in root meristematic tissues, and is induced >2-fold during a 5-h period when mitosis in the root cap meristem is increased. Expression of PsFut1 antisense mRNA in pea hairy roots under the control of the CaMV35S promoter, which exhibits meristem localized expression in pea root caps, resulted in a 50–60% reduction in meristem localized endogenous PsFut1 mRNA expression measured using whole mount in situ hybridization. Changes in gross levels of cell wall fucosylated xyloglucan were not detected, but altered surface localization patterns were detected using whole mount immunolocalization with CCRC-M1, an antibody that recognizes fucosylated xyloglucan. Emerging hairy roots expressing antisense PsFut1 mRNA appeared normal macroscopically but scanning electron microscopy of tissues with altered CCRC-M1 localization patterns revealed wrinkled, collapsed cell surfaces. As individual border cells separated from the cap periphery, cell death occurred in correlation with extrusion of cellular contents through breaks in the wall

Perspectives on the use of transcriptomics to advance biofuels

As a field within the energy research sector, bioenergy is continuously expanding. Although much has been achieved and the yields of both ethanol and butanol have been improved, many avenues of research to further increase these yields still remain. This review covers current research related with transcriptomics and the application of this high-throughput analytical tool to engineer both microbes and plants with the penultimate goal being better biofuel production and yields. The initial focus is given to the responses of fermentative microbes during the fermentative production of acids, such as butyric acid, and solvents, including ethanol and butanol. As plants offer the greatest natural renewable source of fermentable sugars within the form of lignocellulose, the second focus area is the transcriptional responses of microbes when exposed to plant hydrolysates and lignin-related compounds. This is of particular importance as the acid/base hydrolysis methods commonly employed to make the plant-based cellulose available for enzymatic hydrolysis to sugars also generates significant amounts of lignin-derivatives that are inhibitory to fermentative bacteria and microbes. The article then transitions to transcriptional analyses of lignin-degrading organisms, such as Phanerochaete chrysosporium, as an alternative to acid/base hydrolysis. The final portion of this article will discuss recent transcriptome analyses of plants and, in particular, the genes involved in lignin production. The rationale behind these studies is to eventually reduce the lignin content present within these plants and, consequently, the amount of inhibitors generated during the acid/base hydrolysis of the lignocelluloses. All four of these topics represent key areas where transcriptomic research is currently being conducted to identify microbial genes and their responses to products and inhibitors as well as those related with lignin degradation/formation.clos