胎盤抽出物がヒト骨肉腫細胞株Saos-2の細胞増殖、I型コラーゲン産生およびALP分泌に及ぼす効果

Porcine placenta extract (P-placenta) is widely applied in medicine and cosmetics. However, few studies have examined the effect of the extract on the cellular behavior of the osteoblastic cell line Saos-2. Here, we demonstrated that P-placenta enhances the proliferation, collagen type I production, and alkaline phosphatase (ALP) secretion of Saos-2 in vitro. Proliferation of Saos-2 was assessed by MTT and DNA synthesisassays. Type I collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. The cells were treated with/without 20, 200 and 2000 g/ml of P-placenta for 24 h. We found that 200 g/ml P-placenta significantly induced the proliferation of Saos-2 and enhanced type I collagen production and ALP secretion. The results indicate that P-placenta controls the cellular behavior of osteoblasts,resulting in the secretion of early bone-related biomarkers

The Risk of Liquefaction Associated with Water Head Fluctuation in the Low-lying Area of Tokyo

 In the wide area of the eastern part of Tokyo, the ground level is less than mean sea level. This area is more vulnerable to disasters than other areas. If large flood damage such as storm surge should occur in this area, the disaster would be a long-term catastrophe. On the coast of Tokyo Bay, countermeasures have been taken by tide embankments and floodgates. However, considering the damage scale when it occurs, an analysis in this area is very important. In this area, ground settlement occurred and groundwater head dropped because groundwater excessively withdrew by the industrial purpose during the period of economic growth. Currently, the groundwater head recovers and the ground settlement has been subsided. However, due to the groundwater head fluctuation, pore water pressure distribution had been different from hydrostatic pressure distribution. Therefore, in the analysis in this area, it is necessary to consider past groundwater head fluctuation. In this research, the ground settlement and the distribution of pore water pressure are simulated from groundwater level fluctuation over the past 100 years. Then, we conducted the seismic analysis by input the distribution of effective stress calculating from the simulated ground water pressure. The sites analyzed in this research are Tokyo Sea Life Park at the mouth of Arakawa River