O-C Study of 545 Lunar Occultations from 13 Double Stars

International audienceIn this article, we have studied the reports of lunar occultations by this project observation's teams (named APTO) in comparison with other observations of the objects. Thirteen binary stars were selected for this study. All the previous observations of these stars were also collected. Finally, an analysis of O-C of all reports were performed

Optical properties of microphytobenthic biofilms (MPBOM): Biomass retrieval implication

International audienc

A novel chimeric recombinant protein PDHB-P80 of Mycoplasma agalactiae as a potential diagnostic tool

The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC main workbench 5.5 software. Several linkers and three dimensional structure of PDHB-P80 were compared to the native PDHB and analyzed to select a proper one for expression. The fusion gene sequence was optimized and synthesized in pMAT cloning vector. The synthetic pMAT-pdhb-p80 was digested using Bam HI and Sal I restriction enzymes and ligated into pMAL-p5X expression vector. The pMAL-pdhb-p80 construct was transfected into E.coli BL21 strain cells and expressed protein were purified using amylose resin. and the purified protein was analyzed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. In silico analysis demonstrated that fusion proteins using IgG4 middle hinge (CPSCP) with TM-score of 0.99 showed the higher similarity between three dimensional structure of PDHB before and after fusion with high antigenic region of P80. Successful cloning verified by PCR colony, double digestion and sequence analysis. Besides, SDS-PAGE analysis and Western blotting indicated and confirmed the expression of intact recombinant chimeric protein MBP-PDHB-P80 along with some truncated forms of the recombinant protein. it could be concluded that the fusion construct has a potential for serodiagnostic assay in future studies.Malihe Akbarzadeh-Niaki, Abdollah Derakhshandeh, Nasrin Kazemipour, Vida Eraghi, Farhid Hemmatzade

Pulvérisation ciblée sur les tâches d’adventices détectées par drône en colza

National audienceL’idée de cartographier les zones infestées par les adventices pour restreindre la surface traitée, et donc les quantités d’herbicides utilisées, méritait d’être testée. Depuis l’acquisition des données par drone, en passant par le traitement des images, jusqu’à l’exploitation de la carte par le pulvérisateur au champ. C’est pourquoi, Terres Inovia a décidé de collaborer avec AIRINOV pour conduire un essai sur colza en vue de la pulvérisation ciblée sur les tâches d’adventices détectées préalablement par drone. Cette étude exploratoire avait pour objectif de vérifier l’intérêt, la faisabilité, la pertinence et l’efficacité d’une telle technique. Elle s’inscrit dans le projet européen H2020 « IWMPRAISE ». Pour évaluer la faisabilité, l’ensemble de la chaine d’acquisition, de traitement et d’exploitation au champ a été suivie au cours de l’automne 2018 sur une portion de champ de colza semé à large écartement (60 cm). Pour juger de la pertinence du service, les résultats obtenus par drone ont été confrontés à des relevés de flore sur des placettes dédiées. Enfin, l’efficacité du désherbage a été estimée a posteriori et le gain environnemental lié à la réduction de la surface traitée a été quantifié. Mapping weed infested zones on the field to reduce herbicide applications is an idea which must be tested. From the pictures acquisitions by UAV flight, an infestation map of the field is created and it drives the boom to treat only these infested zones. Terres Inovia and AIRINOV decided to realize a trial on oilseed rape to check if this technic is interesting and feasible. This trial belongs to the H2020 project “IWMPRAISE”. To evaluate the feasibility, all the chain was followed during autumn 2018 on an oilseed rape field sowed at large width (60 cm). To estimate the interest, the results obtained by the UAV flight were compared to weed density measures on the field. The efficiency of the spot weeding was estimated a posteriori and the environmental benefit (reduction of treated surface) was quantified

Synergy Between Hyperspectral (HYSPEX), Multispectral (SPOT 6/7, Sentinel-2) Remotely Sensed Data and LiDAR Data for Mapping the Authie Estuary (France)

International audienceThe Authie estuary, located at the eastern part of the English Channel is of environmental, ecological, economic and societal importance. With the intention to better understand the sediment dynamic it is important to better assess the role of sediment dynamics including erosion, stabilization and sediment reworking processes which is challenging in such complex environment. It is also important to consider biogenic components such as the microphytobenthos (MPB) distribution, as the primary productivity may play an important role with the bio-stabilization process. As a consequence, there is a crucial need to provide a synoptic overview of inherent bio-physical characteristics of sediments (i.e., composition, water content, grain-size, and biomass) in estuarine environment by generating precise quantitative maps for predicting in a second step estuarine evolution by including sediment transport, sedimentation rates, coastal flows processes and sea level rise caused by climate change for instance. The use of the remote sensing technology is increasingly used for mapping estuarine and coastal environments by providing a synoptic overview of bio-physical characteristics of sediments. In that sense, the combination between remote sensing imaging, topographic data (LiDAR) and in situ measurements is suitable for improving our understanding of sediment dynamics with respect to physical and biological forcings. The main objective of this study is to demonstrate that the synergy between multispectral (i.e., SPOT 6–7 [1.5 m/pixel]; Sentinel-2, 10–60 m/pixel, 5–10 days)”, hyperspectral [Hyspex, 70 cm/pixel, 160 spectral bands] remote sensing images may be suitable for generating both reliable sedimentary and primary productivity budgets; at least for surficial sediments. All presented data were acquired during the same day (09/21/2017) in the framework TéléEST, CPER MARCO and CNRS-OMPBI projects