How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels

Nitrogen (N) is one of the most important nutrients for plants and nitric oxide (NO) as a signaling plant growth regulator involved in nitrogen assimilation. Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources of nitrogen is vitally important for increasing nitrogen use efficiency (NUE). This study investigated the expression of key genes and enzymes in relation to nitrogen assimilation in two Australian wheat cultivars, a popular high NUE cv. Spitfire and a normal NUE cv. Westonia, under different combinations of nitrogen and sodium nitroprusside (SNP) as the NO donor. Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen. At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia. Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars, while under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected. N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation. Results reveal the possible role of NO and different nitrogen sources on nitrogen assimilation in Triticum aestivum plants

Effect of Gabapentin on Morphine Consumption and Pain after Surgical Debridement of Burn Wounds:A Double-Blind Randomized Clinical Trial Study

Background: Burn pain is recognized as being maximal during therapeutic procedures, and wound debridement can be more painful than the burn injury itself. Uncontrolled acute burn pain increases the stress response and the incidence of chronic pain and associated depression. Although opiates are excellent analgesics, they do not effectively prevent central sensitization to pain. The anticonvulsant gabapentin has been proven effective for treating neuropathic pain in large placebo-controlled clinical trials. Experimental and clinical studies have demonstrated antihyperalgesic effects in models with central neuronal sensitization. It has been suggested that central neuronal sensitization may play an important role in postoperative pain. Objectives: The aim of this study was to investigate the effect of gabapentin on morphine consumption and postoperative pain in burn patients undergoing resection of burn wounds. Patients and Methods: Results: All the enrolled patients were able to complete the study; therefore, data from 50 patients wereanalyzed. The VAS scores at rest andduring movement at 1,4,8,12,16,20, and 24 h after the operation were significantly lower in the gabapentin group than in the placebo group (P < 0.05). Morphine consumption was significantly lessr in the gabapentin group than in the placebo group (P < 0.05). Sedation scores were similar in the 2 groups at all measured times. There were no differences in adverse effects between the groups. Conclusions: A single oral dose of 1200mg gabapentin resulted in a substantial reduction in postoperative morphine consumption and pain scores after surgical debridement in burn patients

Urban consumers\u2019 attitudes and willingness to pay for functional foods in Iran: A case of dietary sugar

Growing concerns for the incidence of incurable diseases and high costs of health care have attracted consumers to functional foods in the world. These foods are characterized with health improvement, lower risk of disease incidence and less health hazards. The present work examined consumers' attitude and willingness to pay for dietary sugar in Rasht city, Iran. The studied sample included 125 citizens of Rasht in spring and summer of 2016 whose size was determined by Mitchell and Carson approach. Results of contingent valuation method on the basis of one-and-one-half-bound choice model revealed that the descriptive variable of bid had negative, statistically significant impact on the acceptance of bid by participants. In addition, the descriptive variables of respondent's age, educational level, family size, monthly income of the family, record of diabetes in family, healthy purchase attitude, and attitude towards the benefits of dietary sugar had positive, significant influence on bid acceptance. Participants expressed their willingness to pay 35.59% extra for dietary sugar as compared to conventional sugar

The Geochemical, Mineralogical and Characterization of Mineralization Zones In Quartz Diorite, Quartz Monzodiorite and Granite associations (Suite) of Tarik Dareh (Torbat-E Jam) area By Multispectral Data (Rs) and artificial Neural Networks (ann) Methods

Abstract: The granitoid, quartz-diorite and quartz-monzodiorite associations of Tarik Dareh (Torbat-e Jam) and its related placers were examined by XRD, XRF, ICP-MS, RS and ANN methods. The Multispectral data (SAM method) was used for differentiating of lithological units and identification of minerals in the suite. The Envi software provided a means for classification of lithological units into 4 main groups. The geochemical studies showed the granitoid stock is peraluminous (A/CNK = 0.7-1.2) and high K calk alkaline (Na 2 O&lt;K 2 O). The geochemical analysis and trace elements data showed I type, subduction zones and syn-collision VAG type for the granitoid stock in the area. The enrichment during differentiation of magma caused Au, Sb and W anomalous. The Artificial Neural Networks (ANN) method was developed to define favourability zones of occurrences of Au, W and Sb mineralizations and to assess the predictive power of the model. ANN results showed the most of Au anomaly (higher than 4 ppm) can be observed in inner and adjacent of quartzdiorite unit, anomaly of W (higher than 2.2 ppm) in monzogranite and syenogranite ones, but anomaly of Sb (higher than 1.2 ppm) in the quartzdiorite and monzogranite parts

Differential impact of LPG-and PG-deficient Leishmania major mutants on the immune response of human dendritic cells

<div><p>Background</p><p><i>Leishmania major</i> infection induces robust interleukin-12 (IL12) production in human dendritic cells (hDC), ultimately resulting in Th1-mediated immunity and clinical resolution. The surface of <i>Leishmania</i> parasites is covered in a dense glycocalyx consisting of primarily lipophosphoglycan (LPG) and other phosphoglycan-containing molecules (PGs), making these glycoconjugates the likely pathogen-associated molecular patterns (PAMPS) responsible for IL12 induction.</p><p>Methodology/Principal Findings</p><p>Here we explored the role of parasite glycoconjugates on the hDC IL12 response by generating <i>L</i>. <i>major</i> Friedlin V1 mutants defective in LPG alone, (FV1 <i>lpg1-</i>), or generally deficient for all PGs, (FV1 <i>lpg2-</i>). Infection with metacyclic, infective stage, <i>L</i>. <i>major</i> or purified LPG induced high levels of <i>IL12B</i> subunit gene transcripts in hDCs, which was abrogated with FV1 <i>lpg1-</i> infections. In contrast, hDC infections with FV1 <i>lpg2-</i> displayed increased <i>IL12B</i> expression, suggesting other PG-related/<i>LPG2</i> dependent molecules may act to dampen the immune response. Global transcriptional profiling comparing WT, FV1 <i>lpg1-</i>, FV1 <i>lpg2-</i> infections revealed that FV1 <i>lpg1-</i> mutants entered hDCs in a silent fashion as indicated by repression of gene expression. Transcription factor binding site analysis suggests that LPG recognition by hDCs induces IL-12 in a signaling cascade resulting in Nuclear Factor κ B (NFκB) and Interferon Regulatory Factor (IRF) mediated transcription.</p><p>Conclusions/Significance</p><p>These data suggest that <i>L</i>. <i>major</i> LPG is a major PAMP recognized by hDC to induce IL12-mediated protective immunity and that there is a complex interplay between PG-baring <i>Leishmania</i> surface glycoconjugates that result in modulation of host cellular IL12.</p></div

CD8 Cells of Patients with Diffuse Cutaneous Leishmaniasis Display Functional Exhaustion: The Latter Is Reversed, In Vitro, by TLR2 Agonists

Leishmania mexicana (Lm) causes localized (LCL) and diffuse (DCL) cutaneous leishmaniasis. DCL patients have a poor cellular immune response leading to chronicity. It has been proposed that CD8 T lymphocytes (CD8) play a crucial role in infection clearance, although the role of CD8 cytotoxicity in disease control has not been elucidated. Lesions of DCL patients have been shown to harbor low numbers of CD8, as compared to patients with LCL, and leishmanicidal treatment restores CD8 numbers. The marked response of CD8 towards Leishmania parasites led us to analyze possible functional differences between CD8 from patients with LCL and DCL. We compared IFNγ production, antigen-specific proliferation, and cytotoxicity of CD8 purified from PBMC against autologous macrophages (MO) infected with Leishmania mexicana (MOi). Additionally, we analyzed tissue biopsies from both groups of patients for evidence of cytotoxicity associated with apoptotic cells in the lesions. We found that CD8 cell of DCL patients exhibited low cytotoxicity, low antigen-specific proliferation and low IFNγ production when stimulated with MOi, as compared to LCL patients. Additionally, DCL patients had significantly less TUNEL+ cells in their lesions. These characteristics are similar to cellular “exhaustion” described in chronic infections. We intended to restore the functional capacity of CD8 cells of DCL patients by preincubating them with TLR2 agonists: Lm lipophosphoglycan (LPG) or Pam3Cys. Cytotoxicity against MOi, antigen-specific proliferation and IFNγ production were restored with both stimuli, whereas PD-1 (a molecule associated with cellular exhaustion) expression, was reduced. Our work suggests that CD8 response is associated with control of Lm infection in LCL patients and that chronic infection in DCL patients leads to a state of CD8 functional exhaustion, which could facilitate disease spread. This is the first report that shows the presence of functionally exhausted CD8 T lymphocytes in DCL patients and, additionally, that pre-stimulation with TLR2 ligands can restore the effector mechanisms of CD8 T lymphocytes from DCL patients against Leishmania mexicana-infected macrophages

Structural evolution of the Kopeh Dagh fold-and-thrust belt (NE Iran) and interactions with the South Caspian Sea Basin and Amu Darya Basin

We present a detailed stratigraphic and structural study of the Kopeh Dagh fold-and-thrust belt in NE Iran, which is an investigation of the complex polyphased tectonic history of this belt and its links with the adjacent South Caspian Sea and Amu Darya basins. Based on numerous field surveys, a large amount of 2D and 3D seismic data, borehole data and more than 150 new biostratigaphic datings, a new detailed biostratigraphic chart and 4 main regional cross-sections illustrate the importance of lateral facies variations and structural inheritance in the present-day structure of the belt. After the Cimmerian orogeny corresponding to the closure of the Paleotethys Ocean in Late Triassic/Early Jurassic times, a Middle Jurassic post-collisional rifting event was associated with the deposition of one of the main source rocks of the Kopeh Dagh and the Amu Darya Basin (Kashafrud Formation). Following this rifting event, over 7km of sediments were accumulated until the Tertiary above a regional post-Triassic unconformity. The occurrence of local uplifts during the Late Cretaceous-Early Paleocene is interpreted as a consequence of regional-scale modification of plate-slab coupling in the Neotethys subduction zone. The main inversion of the Kopeh Dagh occurred at Late Eocene times, when the far-field deformation developed in Eurasia as a consequence of the locking of the Neo-Tethys subduction. This folding phase is sealed in the western part of the belt by a major Eocene-Oligocene unconformity atthe base of the thick sedimentary series belonging to the South Caspian Sea Basin. The bulk of sedimentary infill in the South Caspian Sea Basin is Oligocene and younger, and it is probably related to syn-compressional downward flexure of the resistant basement basin at the onset of the Alpine phase. In the eastern part of the Kopeh Dagh, this deformation is characterized by Middle Jurassic graben inversion with evidenceof forced folding, short-cuts and as well by larger scale basement uplifts. In contrast, the northwestern part of the belt shows thrust faults involving basement and fault-propagation folds within the sedimentary sequence. The Kopeh Dagh presents tectonic structures that are parallel to the Paleotethys suture zone, which emphasizes the importance of the structural inheritance and inversion processes during the structural evolution of the belt. Finally, a change from a mostly dip-slip to a mostly strike-slip tectonics occurred during the Pliocene within the Kopeh Dagh as a consequence of a major tectonic reorganization in North-East Iran. © 2014 Elsevier Ltd.This study was funded by the Darius Programme and thanks to the strong support and collaboration with the National Iranian Oil Company (NIOC), and in particular the Exploration directorate. Additional funding was provided by the projects ATIZA (CGL2009-09662-BTE) and TECLA (CGL2011-26670).Peer Reviewe

Study of Nitric Oxide production by murine peritoneal macrophages induced by Brucella Lipopolysaccharide

Brueclla is a gram negative bacteria that causes Brucellosis. Lipopolysaccharide (LPS) &quot;, the pathogenic agent of Brucella is composed of O-chain, core oligosaccharide and lipid A. in addition, the structural and biological properties of different LPS extracted from different strains are not identical. The first defense system against LPS is nonspecific immunity that causes macrophage activation. Activated macrophages produce oxygen and nitrogen radicals that enhance the protection against intracellular pathogens.In this experiment LPS was extracted by hot phenol- water procedure and the effect of various LPSs on nitric oxide prodution by peritoneal mouse macrophages was examined.Our results demonstrated that the effect of LPS on nitric oxide production is concentration-dependent we observed the maximum response in concentration of 10-20 microgram per milliliter. Also our results demonstrate that LPS extracted from vaccine Brucella abortus (S 19) had a highe effect on nitric oxide production than the LPS from other strain