Nanostructures: Current uses and future applications in food science

Recent developments in nanoscience and nanotechnology intend novel and innovative applications in the food sector, which is rather recent compared with their use in biomedical and pharmaceutical applications. Nanostructured materials are having applications in various sectors of the food science comprising nanosensors, new packaging materials, and encapsulated food components. Nanostructured systems in food include polymeric nanoparticles, liposomes, nanoemulsions, and microemulsions. These materials enhance solubility, improve bioavailability, facilitate controlled release, and protect bioactive components during manufacture and storage. This review highlights the applications of nanostructured materials for their antimicrobial activity and possible mechanism of action against bacteria, including reactive oxygen species, membrane damage, and release of metal ions. In addition, an overview of nanostructured materials, and their current applications and future perspectives in food science are also presented

In vitro and in silico study of mixtures cytotoxicity of metal oxide nanoparticles to Escherichia coli: a mechanistic approach

Metal oxide nanoparticles (MONPs) are commonly found in the aquatic and terrestrial systems as chemical mixtures. Assessment of cytotoxicity associated with single and combination of MONPs can truly identify the concerned environmental risk. Thus, using Escherichia coli as a test model, in vitro cytotoxicity of 6 single MONPs, 15 binary and 20 tertiary mixtures with equitoxic ratios was evaluated following standard bioassay protocols. Assessment of oxidative stress suggested that the production of reactive oxygen species (ROS) was negligible, and the release of metal zinc ions played an important role in the toxicity of MONP mixtures. From our experimental data points, seven quantitative structure-activity relationships (QSARs) models were developed to model the cytotoxicity of these MONPs, based on our created periodic table-based descriptors and experimentally analyzed Zeta-potential. Two strategic approaches i.e. pharmacological and mathematical hypotheses were considered to identify the mixture descriptors pool for modeling purposes. The stringent validation criteria suggested that the model (Model M4) developed with mixture descriptors generated by square-root mole contribution outperformed the other six models considering validation criteria. While considering the pharmacological approach, the ‘independent action’ generated descriptor pool offered the best model (Model M2), which firmly confirmed that each MONP in the mixture acts through ‘independent action’ to induce cytotoxicity to E. coli instead of fostering an additive, antagonistic or synergistic effect among MONPs. The total metal electronegativity in a specific metal oxide relative to the number of oxygen atoms and metal valence was associated with a positive contribution to cytotoxicity. At the same time, the core count, which gives a measure of molecular bulk and Zeta potential, had a negative contribution to cytotoxicity

Protective Effects of <i>Ammannia baccifera</i> Against CCl₄-Induced Oxidative Stress in Rats

Ammannia baccifera Linn. is commonly used as a traditional medicine in India and China. The antioxidant potential of an ethanolic extract of A. baccifera (EEAB; 250 mg/kg and 500 mg/kg) was evaluated against CCL4-induced toxicity in rats. Antioxidant activity was assessed by measuring the enzymatic and non-enzymatic antioxidants. Phytochemical constituents of EEAB were also analyzed by using UHPLC-QTOF-MS. EEAB treatment markedly reduced CCl4 effects on lipid peroxidation, cholesterol, triacylglycerides, and protein carbonyls. It increased the levels of phospholipids, total sulfhydryl, and antioxidant enzymes, which were reduced by CCl4 intoxication. Treatment with EEAB significantly alleviated the CCl4 effect on non-enzymatic antioxidants. Isoenzyme pattern analyses revealed that significant alterations in superoxide dismutase (SOD1), glutathione peroxidase (GPx2, GPx3), and catalase (CAT) occurred in rats that were exposed to CCl4 and restored post EEAB treatment. Moreover, CCl4-induced down regulation of SOD, CAT, and GPx gene expression was conversely counteracted by EEAB. Its bioactivity may be due to its incorporation of major compounds, such as chlorogenic acid, quercetin, protocatechuic acid, lamioside, crocetin, and khayasin C. These results suggest that EEAB may be used as a potent antioxidant and hepatoprotective agent since it is a rich source of flavonoids and phenolic compounds

Photoinactivation of <i>Escherichia coli</i> by Sulfur-Doped and Nitrogen–Fluorine-Codoped TiO₂ Nanoparticles under Solar Simulated Light and Visible Light Irradiation

Titanium dioxide (TiO₂) is one of the most widely used photocatalysts for the degradation of organic contaminants in water and air. Visible light (VL) activated sulfur-doped TiO₂ (S-TiO₂) and nitrogen–fluorine-codoped TiO₂ (N–F-TiO₂) were synthesized by sol–gel methods and characterized. Their photoinactivation performance was tested against <i>Escherichia coli</i> under solar simulated light (SSL) and VL irradiation with comparison to commercially available TiO₂. Undoped Degussa-Evonik P-25 (P-25) and Sigma-TiO₂ showed the highest photocatalytic activity toward <i>E. coli</i> inactivation under SSL irradiation, while S-TiO₂ showed a moderate toxicity. After VL irradiation, Sigma-TiO₂ showed higher photoinactivation, whereas S-TiO₂ and P-25 showed moderate toxicity. Oxidative stress to <i>E. coli</i> occurred via formation of hydroxyl radicals leading to lipid peroxidation as the primary mechanism of bacterial inactivation. Various other biological models, including human keratinocytes (HaCaT), zebrafish liver cells (ZFL), and zebrafish embryos were also used to study the toxicity of TiO₂ NPs. In conclusion, N–F-TiO₂ did not show any toxicity based on the assay results from all the biological models used in this study, whereas S-TiO₂ was toxic to zebrafish embryos under all the test conditions. These findings also demonstrate that the tested TiO₂ nanoparticles do not show any adverse effects in HaCaT and ZFL cells