Small-scale biogeographic patterns of benthic bacterial and ciliate communities in the saline ponds of Lake MacLeod, North-Western Australia

This biogeographical thesis tests for the presence of taxa-area and distance-decay relationships, which are common among macrobionts, in prokaryotic (bacterial) and micro-eukaryotic (ciliate) communities. Microbial biogeographical patterns may be distinct because of the high abundances, diversity and dispersal capabilities of microbes, in comparison to macrobionts. The Northern Ponds of Lake MacLeod, north-western Australia, provide an ideal location to address this topic, because the ponds are effectively hydrogeomorphologically identical, other than in surface area, and biotic histories can be assumed to differ only according to distance of separation. This means that hypotheses concerning species-sorting and neutral processes on microbial assemblages can be tested in a natural setting. Characteristics of the physical environment were determined using bathymetric and hydrodynamic surveys in eight ponds. For each pond, evaporative outflow was determined using pan evaporation rates, and the hydrodynamic characteristics of each pond were described by measuring water flowing out of the ponds. Four pond morphotypes were distinguished on the basis of physical characteristics (surface area, volume and mean depth) and hydrodynamic properties (water residence time and percentage of evaporative loss). For ionic and nutrient variation within and between the ponds, concentrations were expected to vary based on residence time of the brine within the ponds, evapoconcentration and subsequent precipitation of mineral phases. The water chemistry was found to be similar to seawater, with major ionic ratios remaining rather constant throughout each pond. Cygnet Pond differed from the other ponds in that it was enriched in Mg and Ca and depleted in K. Sediment characteristics were also investigated by microscopy. Six sediment types were described based on the particles found in each sample. There were no clear relationships between sedimentology types and water chemistry, and between each of these and the pond morphotypes. The bacterial and ciliate biofilm communities were analysed using DNA community fingerprinting methods, and constrained to the above environmental parameters using redundancy analyses. Distance-decay relationships were found for the bacterial communities within the ponds, and occurred at relatively short distances ( The results therefore provide evidence that microbial bacteria and ciliate biofilm communities can conform to observed biogeographic patterns for macrobionts, although neither community displayed taxa-area relationships. The communities differed in that a distance-decay relationship was only found in the bacterial community, where ciliate taxa are distributed ‘patchily’, and not as a function of distance. An alternative model is proposed for the bacteria and ciliate communities of Lake MacLeod; each pond, because of their isolation from one another, is influenced by stochastic events which differentiate the ponds via ecological drift. This thesis demonstrates that these microbial communities are capable of having complex biogeographies, and that processes such as ecological drift may be important determinants of their structure

Assessment of post-wildfire erosion risk and effects on water quality in south-western Australia

Investigations of wildfire impact on water resources have escalated globally over the last decade owing to an awareness of climate-related vulnerabilities. Within Australia, research into post-wildfire erosion has focused on water supply catchments in the south-eastern region. Here, we examine post-wildfire erosion risk and its potential for water quality impacts in a catchment in south-western Australia. The catchment of the Harvey River, which drains from forested escarpments onto an agricultural coastal plain and into valuable coastal wetlands, was burnt by wildfire in 2016. The aims of this study were to determine erosion risk across contrasting landforms and variable fire severity, using the Revised Universal Soil Loss Equation (RUSLE), and to determine whether post-fire water quality impacts could be detected at permanent river monitoring stations located on the coastal plain. RUSLE outputs showed erosion hot-spots at intersections of steep terrain and high fire severity and that these areas were confined to forested headwaters and coastal dunes. Monthly water quality data showed conspicuous seasonal patterns, but that sampling frequency was temporally too coarse to pick up predicted event-related effects, particularly given that the pre-existing monitoring sites were distal to the predicted zone of contamination. © IAWF 2020 Open Access

Intestinal Microbiome Richness of Coral Reef Damselfishes (Actinopterygii: Pomacentridae)

Fish gastro-intestinal system harbors diverse microbiomes that affect the host's digestion, nutrition, and immunity. Despite the great taxonomic diversity of fish, little is understood about fish microbiome and the factors that determine its structure and composition. Damselfish are important coral reef species that play pivotal roles in determining algae and coral population structures of reefs. Broadly, damselfish belong to either of two trophic guilds based on whether they are planktivorous or algae-farming. In this study, we used 16S rRNA gene sequencing to investigate the intestinal microbiome of 5 planktivorous and 5 algae-farming damselfish species (Pomacentridae) from the Great Barrier Reef. We detected Gammaproteobacteria ASVs belonging to the genus Actinobacillus in 80% of sampled individuals across the 2 trophic guilds, thus, bacteria in this genus can be considered possible core members of pomacentrid microbiomes. Algae-farming damselfish had greater bacterial alpha-diversity, a more diverse core microbiome and shared 35 ± 22 ASVs, whereas planktivorous species shared 7 ± 3 ASVs. Our data also highlight differences in microbiomes associated with both trophic guilds. For instance, algae-farming damselfish were enriched in Pasteurellaceae, whilst planktivorous damselfish in Vibrionaceae. Finally, we show shifts in bacterial community composition along the intestines. ASVs associated with the classes Bacteroidia, Clostridia, and Mollicutes bacteria were predominant in the anterior intestinal regions while Gammaproteobacteria abundance was higher in the stomach. Our results suggest that the richness of the intestinal bacterial communities of damselfish reflects host species diet and trophic guild

Ocean connectivity and habitat characteristics predict population genetic structure of seagrass in an extreme tropical setting

Understanding patterns of gene flow and processes driving genetic differentiation is important for a broad range of conservation practices. In marine organisms, genetic differentiation among populations is influenced by a range of spatial, oceanographic, and environmental factors that are attributed to the seascape. The relative influences of these factors may vary in different locations and can be measured using seascape genetic approaches. Here, we applied a seascape genetic approach to populations of the seagrass, Thalassia hemprichii, at a fine spatial scale (~80 km) in the Kimberley coast, western Australia, a complex seascape with strong, multidirectional currents greatly influenced by extreme tidal ranges (up to 11 m, the world\u27s largest tropical tides). We incorporated genetic data from a panel of 16 microsatellite markers, overwater distance, oceanographic data derived from predicted passive dispersal on a 2 km-resolution hydrodynamic model, and habitat characteristics from each meadow sampled. We detected significant spatial genetic structure and asymmetric gene flow, in which meadows 12–14 km apart were less connected than ones 30–50 km apart. This pattern was explained by oceanographic connectivity and differences in habitat characteristics, suggesting a combined scenario of dispersal limitation and facilitation by ocean current with local adaptation. Our findings add to the growing evidence for the key role of seascape attributes in driving spatial patterns of gene flow. Despite the potential for long-distance dispersal, there was significant genetic structuring over small spatial scales implicating dispersal and recruitment bottlenecks and highlighting the importance of implementing local-scale conservation and management measures

Australian vegetated coastal ecosystems as global hotspots for climate change mitigation

Policies aiming to preserve vegetated coastal ecosystems (VCE; tidal marshes, mangroves and seagrasses) to mitigate greenhouse gas emissions require national assessments of blue carbon resources. Here, we present organic carbon (C) storage in VCE across Australian climate regions and estimate potential annual CO2 emission benefits of VCE conservation and restoration. Australia contributes 5–11% of the C stored in VCE globally (70–185 Tg C in aboveground biomass, and 1,055–1,540 Tg C in the upper 1 m of soils). Potential CO2 emissions from current VCE losses are estimated at 2.1–3.1 Tg CO2-e yr-1, increasing annual CO2 emissions from land use change in Australia by 12–21%. This assessment, the most comprehensive for any nation to-date, demonstrates the potential of conservation and restoration of VCE to underpin national policy development for reducing greenhouse gas emissions

Australian vegetated coastal ecosystems as global hotspots for climate change mitigation

© 2019, The Author(s). Policies aiming to preserve vegetated coastal ecosystems (VCE; tidal marshes, mangroves and seagrasses) to mitigate greenhouse gas emissions require national assessments of blue carbon resources. Here, we present organic carbon (C) storage in VCE across Australian climate regions and estimate potential annual CO2 emission benefits of VCE conservation and restoration. Australia contributes 5–11% of the C stored in VCE globally (70–185 Tg C in aboveground biomass, and 1,055–1,540 Tg C in the upper 1 m of soils). Potential CO2 emissions from current VCE losses are estimated at 2.1–3.1 Tg CO2-e yr-1, increasing annual CO2 emissions from land use change in Australia by 12–21%. This assessment, the most comprehensive for any nation to-date, demonstrates the potential of conservation and restoration of VCE to underpin national policy development for reducing greenhouse gas emissions

Australian vegetated coastal ecosystems as global hotspots for climate change mitigation

Unidad de excelencia María de Maeztu MdM-2015-0552Policies aiming to preserve vegetated coastal ecosystems (VCE; tidal marshes, mangroves and seagrasses) to mitigate greenhouse gas emissions require national assessments of blue carbon resources. Here, we present organic carbon (C) storage in VCE across Australian climate regions and estimate potential annual CO emission benefits of VCE conservation and restoration. Australia contributes 5-11% of the C stored in VCE globally (70-185 Tg C in aboveground biomass, and 1,055-1,540 Tg C in the upper 1 m of soils). Potential CO emissions from current VCE losses are estimated at 2.1-3.1 Tg CO-e yr, increasing annual CO emissions from land use change in Australia by 12-21%. This assessment, the most comprehensive for any nation to-date, demonstrates the potential of conservation and restoration of VCE to underpin national policy development for reducing greenhouse gas emissions

Community composition of carrion-breeding blowflies (Diptera: Calliphoridae) along an urban gradient in south-eastern Australia

Urbanisation is a process that results in rapid modification of the natural environment, dramatically altering community structure. Blowflies (Diptera: Calliphoridae) are common inhabitants of the urban ecosystem, although little is understood about their distributions or habitat preferences within the urban environment. Blowflies require carrion for development, and as carrion is an ephemeral resource, the effect of urbanisation on these flies may be expected to differ from insects that utilise more uniformly distributed resources. In this study, blowflies were captured at various locations along an urban gradient in the region of Sydney, Australia, during summer and winter. Four habitat categories were sampled: bush, farm, suburban and urban. Using analysis of similarities (ANOSIM), calliphorid assemblages differed between all habitats, except urban and suburban. Species associations with environmental variables were also analysed using canonical correspondence analysis (CCA). Calliphorid abundances were lower in the winter trapping period, compared with the summer trapping period. Chrysomya was the most abundant genus during summer, whilst Calliphora was the most abundant genus during the winter. Some species also displayed temporal changes in their habitat preferences and synanthropic behaviour. Other species were only present in the urban habitats during winter, suggesting that they rely on urban refuges at this time of year. The ecological effects of urbanisation were clearly observed in the present study, since three distinct calliphorid assemblages were found at three different levels of urbanisation within the urban gradient. This study provides information on blowfly responses to urbanisation of use to forensic and ecological entomologists

Bacterial and ciliate biofilm community structure at different spatial levels of a salt lake meta-community

Meta-communities are assembled along an ecological scale that determines local and regional diversity. Spatial patterns have been detected in planktonic bacterial communities at distances \u3c20 \u3em, but little is known about the occurrence of similar variation for other microbial groups and changes in microbial meta-community assembly at different levels of a meta-community. To examine this variation, the biofilm of eight saline ponds were used to investigate processes shaping diversity within ponds (β) and between ponds (δ). Bacterial and ciliate communities were assessed using ARISA and T-RFLP respectively, while diversity partitioning methods were used to examine the importance of taxonomic turnover and variation partitioning was used to distinguish spatial from environmental determinants. The results show that turnover is important for determining β- and δ-diversity of biofilms. Spatial factors are important drivers of bacterial β-diversity but were unimportant for ciliate β-diversity. Environmental variation was a strong determinant of bacterial and ciliate δ-diversity, suggesting sorting processes are important for assembling pond communities. Determinants of diversity in bacteria are not universal for ciliates, suggesting higher functional redundancy of bacteria or the greater niche breadth of ciliates may be important in discriminating assembly processes between the two organisms