Participatory development of storymaps to visualize the spatiotemporal dynamics and impacts of extreme flood events for disaster preparedness

Floods are one of the costliest natural hazards in Switzerland and worldwide. Therefore, society is confronted with questions about protecting people and assets from flood risks. Key instruments are protective measures, land use regulations, spatial planning, and the interventions by civil protection units if flood magnitudes exceed the protection standards. Both prevention and preparedness require risk awareness from professionals, politicians, and the public. Risk awareness is generally high after an event and low after a period without major events. However, the rarity of extreme flood events limits learning from flood events. The training of intervention forces who should manage flood events with magnitudes beyond hitherto observed flood events requires a comprehensive description and visualization of the flood processes and their impacts. To address this, together with stakeholders and civil protection and intervention planning experts, we co-developed a new way to visualize the spatiotemporal dynamics of extreme flood events and thereby communicate their impacts using dynamical flood storymaps. We selected physically plausible precipitation scenarios from reforecasts to develop storylines of extreme river flood events and their socioeconomic impacts in Switzerland. The co-development process revealed which information is relevant to potential users and how it must be presented. It is shown that storylines of extreme events presented as storymaps are a valuable tool to communicate scientific results in a way that allows practitioners to gain relevant information for their work. Therefore, we built an interactive online tool (www.flooddynamics.ch), enabling the user to analyze the spatiotemporal unfolding of flood events in Switzerland from the start of precipitation to the recession of the flood. The visualization includes maps of inundated areas at hourly timesteps and the related impacts in terms of affected persons, buildings, roads, and infrastructure. Such a temporally explicit (dynamic) representation of extreme events in storymaps, in contrast to static hazard maps, which are commonly used today, is favorable for emergency intervention planning and training and thus for awareness creation and better disaster preparedness

A snow and glacier hydrological model for large catchments – case study for the Naryn River, central Asia

In this paper we implement a degree day snowmelt and glacier melt model in the Dynamic fluxEs and ConnectIvity for Predictions of HydRology (DECIPHeR) model. The purpose is to develop a hydrological model that can be applied to large glaciated and snow-fed catchments yet is computationally efficient enough to include model uncertainty in streamflow predictions. The model is evaluated by simulating monthly discharge at six gauging stations in the Naryn River catchment (57 833 km2) in central Asia over the period 1951 to a variable end date between 1980 and 1995 depending on the availability of discharge observations. The spatial distribution of simulated snow cover is validated against MODIS weekly snow extent for the years 2001–2007. Discharge is calibrated by selecting parameter sets using Latin hypercube sampling and assessing the model performance using six evaluation metrics. The model shows good performance in simulating monthly discharge for the calibration period (NSE is 0.74&lt;NSE&lt;0.87) and validation period (0.7&lt;NSE&lt;0.9), where the range of NSE values represents the 5th–95th percentile prediction limits across the gauging stations. The exception is the Uch-Kurgan station, which exhibits a reduction in model performance during the validation period attributed to commissioning of the Toktogul reservoir in 1975 which impacted the observations. The model reproduces the spatial extent in seasonal snow cover well when evaluated against MODIS snow extent; 86 % of the snow extent is captured (mean 2001–2007) for the median ensemble member of the best 0.5 % calibration simulations. We establish the present-day contributions of glacier melt, snowmelt and rainfall to the total annual runoff and the timing of when these components dominate river flow. The model predicts well the observed increase in discharge during the spring (April–May) associated with the onset of snow melting and peak discharge during the summer (June, July and August) associated with glacier melting. Snow melting is the largest component of the annual runoff (89 %), followed by the rainfall (9 %) and the glacier melt component (2 %), where the values refer to the 50th percentile estimates at the catchment outlet gauging station Uch-Kurgan. In August, glacier melting can contribute up to 66 % of the total runoff at the highly glacierized Naryn headwater sub-catchment. The glaciated area predicted by the best 0.5 % calibration simulations overlaps the Landsat observations for the late 1990s and mid-2000s. Despite good predictions for discharge, the model produces a large range of estimates for the glaciated area (680–1196 km2) (5th–95th percentile limits) at the end of the simulation period. To constrain these estimates further, additional observations such as glacier mass balance, snow depth or snow extent should be used directly to constrain model simulations.</p

KAP1 targets actively transcribed genomic loci to exert pleomorphic effects on RNA polymerase II activity

KAP1 (KRAB-associated protein 1) is best known as a co-repressor responsible for inducing heterochromatin formation, notably at transposable elements. However, it has also been observed to bind the transcription start site of actively expressed genes. To address this paradox, we characterized the protein interactome of KAP1 in the human K562 erythro-leukaemia cell line. We found that the regulator can associate with a wide range of nucleic acid binding proteins, nucleosome remodellers, chromatin modifiers and other transcription modulators. We further determined that KAP1 is recruited at actively transcribed polymerase II promoters, where its depletion resulted in pleomorphic effects, whether expression of these genes was normally constitutive or inducible, consistent with the breadth of possible KAP1 interactors. This article is part of a discussion meeting issue 'Crossroads between transposons and gene regulation'

Bispecific PD1-IL2v and anti-PD-L1 break tumor immunity resistance by enhancing stem-like tumor-reactive CD8⁺ T cells and reprogramming macrophages.

Immunotherapies have shown remarkable, albeit tumor-selective, therapeutic benefits in the clinic. Most patients respond transiently at best, highlighting the importance of understanding mechanisms underlying resistance. Herein, we evaluated the effects of the engineered immunocytokine PD1-IL2v in a mouse model of de novo pancreatic neuroendocrine cancer that is resistant to checkpoint and other immunotherapies. PD1-IL2v utilizes anti-PD-1 as a targeting moiety fused to an immuno-stimulatory IL-2 cytokine variant (IL2v) to precisely deliver IL2v to PD-1 &lt;sup&gt;+&lt;/sup&gt; T cells in the tumor microenvironment. PD1-IL2v elicited substantial infiltration by stem-like CD8 &lt;sup&gt;+&lt;/sup&gt; T cells, resulting in tumor regression and enhanced survival in mice. Combining anti-PD-L1 with PD1-IL2v sustained the response phase, improving therapeutic efficacy both by reprogramming immunosuppressive tumor-associated macrophages and enhancing T cell receptor (TCR) immune repertoire diversity. These data provide a rationale for clinical trials to evaluate the combination therapy of PD1-IL2v and anti-PD-L1, particularly in immunotherapy-resistant tumors infiltrated with PD-1 &lt;sup&gt;+&lt;/sup&gt; stem-like T cells

Assessing the sustainability of water governance systems: the sustainability wheel

We present and test a conceptual and methodological approach for interdisciplinary sustainability assessments of water governance systems based on what we call the sustainability wheel. The approach combines transparent identification of sustainability principles, their regional contextualization through sub-principles (indicators), and the scoring of these indicators through deliberative dialogue within an interdisciplinary team of researchers, taking into account their various qualitative and quantitative research results. The approach was applied to a sustainability assessment of a complex water governance system in the Swiss Alps. We conclude that the applied approach is advantageous for structuring complex and heterogeneous knowledge, gaining a holistic and comprehensive perspective on water sustainability, and communicating this perspective to stakeholders

MontanAqua : Wasserbewirtschaftung in Zeiten von Knappheit und globalem Wandel. Wasserbewirtschaftungsoptionen für die Region Crans-Montana-Sierre im Wallis

Das nationale Forschungsprogramm NFP 61 «Nachhaltige Wassernutzung » des Schweizerischen Nationalfonds hat sich zum Ziel gesetzt, wissenschaftliche Grundlagen zur nachhaltigen Wasserbewirtschaftung in der Schweiz zu liefern. Als Teil dieses Forschungsvorhabens wurde im Rahmen des Projektes MontanAqua die Wasserbewirtschaftung der Region Crans-Montana-Sierre (Wallis) untersucht. Es ging dabei darum, in enger Zusammenarbeit mit den in der Region betroffenen Akteuren nachhaltige Wassernutzungsstrategien für die Zukunft zu entwickeln. MontanAqua hat sich vertieft mit den bestehenden Systemen der Wasserbewirtschaftung auf der regionalen Skala (11 Gemeinden) auseinandergesetzt. Dazu wurden die zukünftigen Auswirkungen der klimatischen und sozioökonomischen Veränderungen einbezogen. Das Forschungsteam analysierte die aktuelle Situation anhand von quantitativen, qualitativen sowie kartografischen Methoden und kombinierte diese mit Modellberechnungen. Für die Modellierung der Zukunft wurden regionale Klimaszenarien und vier mit lokalen Akteuren entwickelte sozioökonomische Szenarien verwendet. Dieser Überblick fasst die Resultate des Projektes MontanAqua zusammen. Fünf wesentliche Fragen werden beantwortet und fünf Kernbotschaften erläutert. Zudem sind Empfehlungen für die Verantwortlichen der regionalen und kantonalen Wasserbewirtschaftung formuliert

Montanaqua: tackling water stress in the alps: water management options in the crans-montana-sierre region (valais)

Transdisciplinary research is considered an appropriate mode of knowledge production in the search for pathways towards a more sustainable governance of natural resources. However, the co-production of new knowledge between scientists of different disciplines and nonacademic stakeholders is a challenge that requires novel research designs, methods, and approaches. The MontanAqua team has tackled this challenge by designing and implementing an innovative process of co- production of knowledge. An important element of this process was an assessment and communication tool known as “the sustainability wheel”

The mouse genome displays highly dynamic populations of KRAB-zinc finger protein genes and related genetic units.

KRAB-containing poly-zinc finger proteins (KZFPs) constitute the largest family of transcription factors encoded by mammalian genomes, and growing evidence indicates that they fulfill functions critical to both embryonic development and maintenance of adult homeostasis. KZFP genes underwent broad and independent waves of expansion in many higher vertebrates lineages, yet comprehensive studies of members harbored by a given species are scarce. Here we present a thorough analysis of KZFP genes and related units in the murine genome. We first identified about twice as many elements than previously annotated as either KZFP genes or pseudogenes, notably by assigning to this family an entity formerly considered as a large group of Satellite repeats. We then could delineate an organization in clusters distributed throughout the genome, with signs of recombination, translocation, duplication and seeding of new sites by retrotransposition of KZFP genes and related genetic units (KZFP/rGUs). Moreover, we harvested evidence indicating that closely related paralogs had evolved through both drifting and shifting of sequences encoding for zinc finger arrays. Finally, we could demonstrate that the KAP1-SETDB1 repressor complex tames the expression of KZFP/rGUs within clusters, yet that the primary targets of this regulation are not the KZFP/rGUs themselves but enhancers contained in neighboring endogenous retroelements and that, underneath, KZFPs conserve highly individualized patterns of expression