Myelodysplastic syndrome accompanied by basophilia and eosinophilia with t(5;12)(q31;p13)

The t(5;12)(q31not, vert, similar35;p12not, vert, similar13) is rare among cytogenetically categorized myeloid diseases. Here we describe a case of myelodysplastic syndrome (MDS) with basophilia followed by leukocytosis, basophilia, and eosinophilia with t(5;12)(q31;p13).A 44-year-old man was referred to Tsukuba University Hospital in August 2005, due to severe anemia and thrombocytopenia. Peripheral blood examination showed hemoglobin 4.5 g/dL, with mean corpuscular volume 109 fL, platelets 73 × 109/L, and white blood cells 4.9 × 109/L with 23% basophils, 3% eosinophils, and 0% blasts. Bone marrow was slightly hypocellular, with trilineage dysplasia. Cytogenetic examination of the bone marrow cells revealed a normal karyotype, 46,XY. A diagnosis of myelodysplastic syndrome–refractory anemia with excess blasts type 2 (MDS-RAEB2) was made according to the WHO classification

Production of scFv-Conjugated Affinity Silk Powder by Transgenic Silkworm Technology

Bombyx mori (silkworm) silk proteins are being utilized as unique biomaterials for medical applications. Chemical modification or post-conjugation of bioactive ligands expand the applicability of silk proteins; however, the processes are elaborate and costly. In this study, we used transgenic silkworm technology to develop single-chain variable fragment (scFv)-conjugated silk fibroin. The cocoons of the transgenic silkworm contain fibroin L-chain linked with scFv as a fusion protein. After dissolving the cocoons in lithium bromide, the silk solution was dialyzed, concentrated, freeze-dried, and crushed into powder. Immunoprecipitation analyses demonstrate that the scFv domain retains its specific binding activity to the target molecule after multiple processing steps. These results strongly suggest the promise of scFv-conjugated silk fibroin as an alternative affinity reagent, which can be manufactured using transgenic silkworm technology at lower cost than traditional affinity carriers

Brain Cortical Mapping by Simultaneous Recording of Functional Near Infrared Spectroscopy and Electroencephalograms from the Whole Brain During Right Median Nerve Stimulation

To investigate relationships between hemodynamic responses and neural activities in the somatosensory cortices, hemodynamic responses by near infrared spectroscopy (NIRS) and electroencephalograms (EEGs) were recorded simultaneously while subjects received electrical stimulation in the right median nerve. The statistical significance of the hemodynamic responses was evaluated by a general linear model (GLM) with the boxcar design matrix convoluted with Gaussian function. The resulting NIRS and EEGs data were stereotaxically superimposed on the reconstructed brain of each subject. The NIRS data indicated that changes in oxy-hemoglobin concentration increased at the contralateral primary somatosensory (SI) area; responses then spread to the more posterior and ipsilateral somatosensory areas. The EEG data indicated that positive somatosensory evoked potentials peaking at 22 ms latency (P22) were recorded from the contralateral SI area. Comparison of these two sets of data indicated that the distance between the dipoles of P22 and NIRS channels with maximum hemodynamic responses was less than 10 mm, and that the two topographical maps of hemodynamic responses and current source density of P22 were significantly correlated. Furthermore, when onset of the boxcar function was delayed 5–15 s (onset delay), hemodynamic responses in the bilateral parietal association cortices posterior to the SI were more strongly correlated to electrical stimulation. This suggests that GLM analysis with onset delay could reveal the temporal ordering of neural activation in the hierarchical somatosensory pathway, consistent with the neurophysiological data. The present results suggest that simultaneous NIRS and EEG recording is useful for correlating hemodynamic responses to neural activity

A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae)

Kojima, Hiroaki, Morimoto, Katsura (2003): A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae). The Coleopterists Bulletin 57 (4): 383-389, DOI: 10.1649/567, URL: http://dx.doi.org/10.1649/56

Fig. 14 in A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae)

Fig. 14. Distribution of the subgenus Lignyodes.Published as part of <i>Kojima, Hiroaki & Morimoto, Katsura, 2003, A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae), pp. 383-389 in The Coleopterists Bulletin 57 (4)</i> on page 387, DOI: 10.1649/567, <a href="http://zenodo.org/record/10103713">http://zenodo.org/record/10103713</a&gt

Fig. 15 in A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae)

Fig. 15. Distribution of Lignyodes japonicus.Published as part of <i>Kojima, Hiroaki & Morimoto, Katsura, 2003, A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae), pp. 383-389 in The Coleopterists Bulletin 57 (4)</i> on page 388, DOI: 10.1649/567, <a href="http://zenodo.org/record/10103713">http://zenodo.org/record/10103713</a&gt

Figs. 2–13 in A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae)

Figs. 2–13. Characters of Lignyodes japonicus. 2) Head, lateral view, male; 3) head, lateral view, female; 4) fore tibia, male; 5) hind tibia, male; 6) fore tibia, female; 7) hind tibia, female; 8) antenna, male; 9) antenna, female; 10) aedeagus; 11) 8th and 9th sternites, male; 12) spermatheca; 13) 8th sternite, female.Published as part of <i>Kojima, Hiroaki & Morimoto, Katsura, 2003, A New Lignyodes Dejean, A New Representative of the Genus and the Tribe Lignyodini from the Eastern Palaearctic Region (Coleoptera: Curculionidae), pp. 383-389 in The Coleopterists Bulletin 57 (4)</i> on page 386, DOI: 10.1649/567, <a href="http://zenodo.org/record/10103713">http://zenodo.org/record/10103713</a&gt