A novel cost effective and high-throughput isolation and identification method for marine microalgae

Background: Marine microalgae are of major ecologic and emerging economic importance. Biotechnological screening schemes of microalgae for specific traits and laboratory experiments to advance our knowledge on algal biology and evolution strongly benefit from culture collections reflecting a maximum of the natural inter- and intraspecific diversity. However, standard procedures for strain isolation and identification, namely DNA extraction, purification, amplification, sequencing and taxonomic identification still include considerable constraints increasing the time required to establish new cultures. Results: In this study, we report a cost effective and high-throughput isolation and identification method for marine microalgae. The throughput was increased by applying strain isolation on plates and taxonomic identification by direct PCR (dPCR) of phylogenetic marker genes in combination with a novel sequencing electropherogram based screening method to assess the taxonomic diversity and identity of the isolated cultures. For validation of the effectiveness of this approach, we isolated and identified a range of unialgal cultures from natural phytoplankton communities sampled in the Arctic Ocean. These cultures include the isolate of a novel marine Chlorophyceae strain among several different diatoms. Conclusions: We provide an efficient and effective approach leading from natural phytoplankton communities to isolated and taxonomically identified algal strains in only a few weeks. Validated with sensitive Arctic phytoplankton, this approach overcomes the constraints of standard molecular characterisation and establishment of unialgal cultures

How does the local wind field control the aerosol distribution in coastal Dronning Maud Land?

Atmospheric circulation patterns and chemical concentrations in firn cores are highly related to each other. Atmospheric winds transport aerosols like sea salt and mineral dust over the globe and redistribute them. Because of this, it is possible to reconstruct atmospheric circulation bringing aerosol to Antarctica by analyzing chemical impurities in firn and ice. With these analyses, the gap caused by sparse atmospheric measurements can be filled and this knowledge can then be used to improve the understanding of local and global circulation patterns.Due to a very high accumulation rate (~600 kg/m²*a), coastal Dronning Maud Land (CDML) is a perfect site to conduct these studies.Here, the upper 6m of two firn cores drilled on Halvfaryggen and Sörasen (covering the time interval from 2002- 2007) were analyzed on ionic concentrations. This data was then contrasted to measurements from the air chemistry laboratories at Neumayer (NM) and Kohnenstation (KS), and synoptic measurements from automatic weather stations (distributed in CDML and at NM).The analyses show very different results: Sea salt ions (e.g. Na+) are higher correlated to ions measured in aerosol samples at the air chemistry laboratory at KS than to the one located at NM. In contrast, ions representing mineral dust (e.g. nss-Ca2+) only have a weak correlation over the whole area and time period. Accordingly, the deposition of aerosol is highly dependent on its origin and the topography in coastal Antarctica suggesting different transport pathways for sea level and higher altitude sites

Determining optimal transit charges: The Kiel Canal in Germany

The Kiel Canal in Germany connects ports on the Baltic Sea with the rest of the world and is the most-used artificial waterway in the world. Despite this fact, it generates a balance sheet loss. Revenues, which are mainly generated by the transit charge, do not cover its operating expenses. This situation raises the question: What reforms could be made to make the canal generate a balance sheet profit? In this paper, we focus solely on the canal's revenue. Because the canal is a monopoly that allows, in principle, for perfect price discrimination, we contrast the current charging system with an optimal charging system based on the willingness-to-pay (WTP) approach. We devise a general approach to calculate optimal transit charges and apply it in a case study that includes four different ship types. We conclude that much higher revenues could be generated, on the order of between $5 and$45 million more per year and ship type if the transit charge were based not only on ship size but also on a ship's departure and destination ports

Validity of WTP measures under preference uncertainty

This paper establishes a new method for eliciting Willingness to Pay (WTP) in contingent valuation (CV) studies with an open-ended elicitation format: the Range-WTP method. In contrast to the traditional approach for eliciting Point-WTP, Range-WTP explicitly allows for preference uncertainty in responses. Using data from two novel large-scale surveys on the perception of solar radiation management (SRM), a little-known technique for counteracting climate change, we compare the performance of both methods in the field. In doing so, we use the criterion of theoretical validity and measure the degree to which WTP values are consistent with theoretical expectations. In addition, we analyse the test-retest reliability and stability of our results over time. Our evidence suggests that the Range-WTP method clearly outperforms the Point-WTP method

Efficacy of imidacloprid 10%/moxidectin 1% spot-on formulation (Advocate®) in the prevention and treatment of feline aelurostrongylosis

Background: In three randomized, controlled laboratory efficacy studies, the efficacy in the prevention of patent infections of a topical combination of imidacloprid 10%/moxidectin 1% (Advocate® spot-on formulation for cats, Bayer Animal Health GmbH) against larval stages and immature adults of Aelurostrongylus abstrusus, as well as the treatment efficacy of a single or three monthly treatments against adult A. abstrusus, were evaluated. Methods: Cats were experimentally inoculated with 300–800 third-stage larvae (L3). Each group comprised 8 animals and the treatment dose was 10 mg/kg bodyweight (bw) imidacloprid and 1 mg/kg bw moxidectin in each study. Prevention of the establishment of patent infections was evaluated by two treatments at a monthly interval at three different time points before and after challenge infection. Curative efficacy was tested by one or three treatments after the onset of patency. Worm counts at necropsy were used for efficacy calculations. Results: In Study 1, the control group had a geometric mean (GM) of 28.8 adult nematodes and the single treatment group had a GM of 3.4 (efficacy 88.3%). In Study 2, the control group had a GM of 14.3, the prevention group had a GM of 0 (efficacy 100%), while the treatment group had a GM of 0.1 (efficacy 99.4%). In Study 3, the GM worm burden in the control group was 32.6 compared to 0 in all three prevention groups (efficacy 100% for all of those groups). Conclusions: The monthly administration of Advocate® reliably eliminated early larval stages and thereby prevented lung damage from and patent infections with A. abstrusus in cats. Regarding treatment, a single application of Advocate® reduced the worm burden, but it did not sufficiently clear the infection. In contrast, three monthly treatments were safe and highly efficacious against A. abstrusus

Cohabitation and marriage in Austria

BACKGROUND Although cohabitation has spread rapidly in Austria during the past decades, it is more a prelude than an alternative to marriage. The individualization thesis serves as a conceptual framework for explaining the rise of cohabiting unions. OBJECTIVE Our aim is to understand what motivates people to cohabit and marry from an individualization perspective. The present study was designed to investigate in which ways key notions of the individualization thesis such as commitment, romantic love and risk are reflected in discourses on cohabitation and marriage. METHODS Research is based on data from eight focus group discussions (71 participants) conducted in Vienna, Austria, in 2012. This data was analyzed with the help of qualitative methods. RESULTS The focus group participants regarded cohabitation and marriage as different life course strategies. They felt that young adulthood is a period characterized by uncertain external circumstances, in which people build up commitment in cohabitation without feeling limited in terms of opportunities. As dissolving a cohabiting union entails lower costs, the risk posed by this type of union was considered low. The respondents associated marriage with security and long-term commitment and saw it as an ideal for a later stage in life. They argued that romantic love and individual satisfaction should prevail throughout the entire marriage. Core terms of the individualization thesis - commitment, romantic love, and risk - were perceived differently between cohabitation and marriage. We conclude that the individualization thesis best fits young adulthood and is less relevant for later life stages

Violations of scale compatibility: results from pricing tasks and choice tasks in choice experiments

According to rational choice theory, preference orderings should be invariant with respect to the elicitation procedure. The contingent trade-off model (Tversky et al., 1988), however, argues that if an attribute of decision alterna-tives is also used as response mode (scale compatibility), then the attribute will be weighted more heavily. We propose a two-stage design for a systemat-ic assessment of how scale compatibility impacts willingness to pay (WTP) es-timates in choice experiments. At the first stage, a pricing task is implemented. Respondents face two alternative goods. For one good, the price is given. For the alternative good, respondents state the price (WTP) that makes them indifferent between the alternatives. In the second stage, a choice task is implemented: another group of respondents makes pairwise choices between two alternative goods with the price being one of several attributes. Our empirical findings support the contingent trade-off model, as pricing tasks yield systematically lower WTP estimates than choice tasks. While the trade-offs between the other attributes do not differ between the pricing and the choice task. Thus, in the choice task the weight shifts away from the price attribute but does not change the relative weight of the other attributes

Exploring public perception of solar radiation management

Solar radiation management (SRM) could quickly offset global warming caused by anthropogenic greenhouse gas emissions. Because SRM would have global side effects, it raises not only technological but also political and social concerns. Therefore, SRM research should be accompanied by a global debate that incorporates public perception and concerns into the development and governance of the technology. Our paper provides insight into public perception and explores its underlying patterns using a survey conducted in Germany. The data reveal a differentiated picture. Laboratory research on SRM is broadly approved, whereas field research is much less approved. Immediate deployment is largely rejected. The acceptance of the technology is associated with the belief that climate change is a severe problem and that humans will eventually be able to control nature. It is also determined by the levels of trust in scientists and firms. Among the strongest objections against the technology is the belief that humans should not manipulate nature in the way SRM would. The actual public perception of SRM will, however, evolve along with the ongoing debate between the public, experts, and policymakers