Evaluating Traits Influencing Hybrid Paddy Seed Production using a Double Haploid Production Derived from Kalanamak Parental Combination

Steps in double haploid breeding for genetic improvement of rice genetically diverse parents could reach the heterotic level of hybrid which pinpointed the prospect of anther culture technique in rice. DH lines with earliness, enhanced biomass production, high panicle density and increased yield potential with resistance top stand grain quality could be produced from anther culture of different crosses. DH lines from cv. 'Calrose 76' showed variation with improved agronomic characters e.g. seed number size, panicle size, plant height and tiller number and improved protein content and higher yields. Scientists reported high genetic variability for filled grains panicle -1 , grains yield plant -1 and number of panicles plant -1 among androgenic plantlets derived from scented Indica rice cv. 'Karnal local 95', suggesting the scope of haploid breeding for higher seed yield. Scientists developed 22 outstanding DHLs for high grain quality through anther culture. Scientists confirmed 98 lines to be DHs out of 232 lines recovered from 'Mahyco Hybrid MRP5401' which had dwarf height, early to late flowering and high yield characters. Development of improved DHs for kalanamak tolerance , submergence tolerance , and bacterial blight resistance , have been reported. Scientists recovered genetically uniform dwarf DHs from anther culture of an advance breeding line 'BR802-78-2-1-1'. A few of these DHs showed high fertility status of spikelet with long-bold and long-slender grain

Crowd Behavior Analysis and Classification using Graph Theoretic Approach

Surveillance systems are commonly used for security and monitoring. The need to automate these systems is well understood. To address this issue we introduce the Graph theoretic approach based Crowd Behavior Analysis and Classification System (GCBACS). The crowd behavior is observed based on the motion trajectories of the personnel in the crowd. Optical flow methods are used to obtain the streak lines and path lines of the crowd personnel trajectories. The streak flow is constructed based on the path and streak lines. The personnel and their respective potential vectors obtained from the streak flows are used to represent each frame as a graph. The frames of the surveillance videos are analyzed using graph theoretic approaches. The cumulative variation in all the frames is computed and a threshold based mechanism is used for classification and activity recognition. The experimental results discussed in the paper prove the efficiency and robustness of the proposed GCBACS for crowd behavior analysis and classification

Surveying and Calculation of a Given Area using a Quad-copter

The field of geometry has various applications and calculating the area is one of them and doing it manually is challenging and equally a daunting task. In this paper we propose a method to make use of a flying object with a camera to calculate the area. This can be achieved by augmenting the flying object with a camera that takes pictures and these pictures can be used and we can perform some operations to calculate the area of a given plane in an image. This not only saves time that an entity invests but also saves the amount of money he/she invests in the calculation of the area. We can enhance the calculation by incorporating much faster techniques and methods

Light curves and spectra from a thermonuclear explosion of a white dwarf merger

This is the final version of the article. Available from the publisher via the DOI in this record.Double-degenerate (DD) mergers of carbon-oxygen white dwarfs have recently emerged as a leading candidate for normal Type Ia supernovae (SNe Ia). However, many outstanding questions surround DD mergers, including the characteristics of their light curves and spectra. We have recently identified a spiral instability in the post-merger phase of DD mergers and demonstrated that this instability self-consistently leads to detonation in some cases. We call this the spiral merger SN Ia model. Here, we utilize the SuperNu radiative transfer software to calculate three-dimensional synthetic light curves and spectra of the spiral merger simulation with a system mass of 2.1 from Kashyap et al. Because of their large system masses, both violent and spiral merger light curves are slowly declining. The spiral merger resembles very slowly declining SNe Ia, including SN 2001ay, and provides a more natural explanation for its observed properties than other SN Ia explosion models. Previous synthetic light curves and spectra of violent DD mergers demonstrate a strong dependence on viewing angle, which is in conflict with observations. Here, we demonstrate that the light curves and spectra of the spiral merger are less sensitive to the viewing angle than violent mergers, in closer agreement with observation. We find that the spatial distribution of 56Ni and IMEs follows a characteristic hourglass shape. We discuss the implications of the asymmetric distribution of 56Ni for the early-time gamma-ray observations of 56Ni from SN 2014J. We suggest that DD mergers that agree with the light curves and spectra of normal SNe Ia will likely require a lower system mass.This work is supported in part at the University of Chicago by the National Science Foundation under grants AST-0909132, PHY-0822648 (JINA, Joint Institute for Nuclear Astrophysics), and PHY–1430152 (JINA-CEE, Joint Institute for Nuclear Astrophysics). This work used the Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by National Science Foundation grant number ACI-1053575. Simulations at UMass Dartmouth were performed on a computer cluster supported by NSF grant CNS-0959382 and AFOSR DURIP grant FA9550-10-1-0354. The work of E.G.-B., G.A.-S., and P. L.-A. was partially funded by the MINECO AYA2014-59084- P grant and by the AGAUR. This research has made use of NASA’s Astrophysics Data System and the yt astrophysics analysis software suit

Tumor-derived interleukin-10 as a prognostic factor in stage III patients undergoing adjuvant treatment with an autologous melanoma cell vaccine.

OBJECTIVES: Interleukin-10 (IL-10) downregulates T-cell-mediated immune responses. We studied the association between IL-10 production by freshly isolated melanoma cell suspensions in vitro and overall survival in patients undergoing adjuvant treatment with a vaccine prepared from the same autologous melanoma cells modified with a hapten, dinitrophenyl (DNP). METHODS: Forty-four patients with cutaneous melanoma (29 stage III and 15 stage IV) were prospectively evaluated. Tumor cells were extracted from metastatic deposits for production of DNP-modified autologous melanoma cell vaccine. Small aliquots of the melanoma cell suspensions were separated prior to vaccine processing and cultured overnight for IL-10 production. Based on a blind assessment of the distribution of IL-10 levels in the culture supernatants, a cutoff of 200 pg/ml was used to define high versus low IL-10 producers. Cox regression model was used for multivariate analysis. Overall survival was calculated using the Kaplan-Meier method, and survival curves were compared with the log-rank test. RESULTS: Out of 44 patients, 29 were low and 15 were high IL-10 producers. The median OS was significantly worse for high compared with low IL-10 producers (10.5 months vs. 42 months; P = 0.022). In stage III patients, the multivariate hazard ratio for high versus low IL-10 producers was 2.92 (95% CI, 1.04-8.20; P = 0.041). The corresponding hazard ratio in stage IV patients was 0.92 (95% CI, 1.04-8.20; P = 0.888). CONCLUSIONS: High IL-10 production in the tumor microenvironment could be a determinant of clinical outcomes in stage III melanoma patients receiving autologous melanoma cell vaccine

Ameliorative Effects of Dimetylthiourea and N-Acetylcysteine on Nanoparticles Induced Cyto-Genotoxicity in Human Lung Cancer Cells-A549

We study the ameliorative potential of dimetylthiourea (DMTU), an OH• radical trapper and N-acetylcysteine (NAC), a glutathione precursor/H2O2 scavenger against titanium dioxide nanoparticles (TiO2-NPs) and multi-walled carbon nanotubes (MWCNTs) induced cyto-genotoxicity in cultured human lung cancer cells-A549. Cytogenotoxicity was induced by exposing the cells to selected concentrations (10 and 50 µg/ml) of either of TiO2-NPs or MWCNTs for 24 h. Anti-cytogenotoxicity effects of DMTU and NAC were studied in two groups, i.e., treatment of 30 minutes prior to toxic insult (short term exposure), while the other group received DMTU and NAC treatment during nanoparticles exposure, i.e., 24 h (long term exposure). Investigations were carried out for cell viability, generation of reactive oxygen species (ROS), micronuclei (MN), and expression of markers of oxidative stress (HSP27, CYP2E1), genotoxicity (P53) and CYP2E1 dependent n- nitrosodimethylamine-demethylase (NDMA-d) activity. In general, the treatment of both DMTU and NAC was found to be effective significantly against TiO2-NPs and MWCNTs induced cytogenotoxicity in A549 cells. Long-term treatment of DMTU and NAC during toxic insults has shown better prevention than short-term pretreatment. Although, cells responded significantly to both DMTU and NAC, but responses were chemical specific. In part, TiO2-NPs induced toxic responses were mediated through OH• radicals generation and reduction in the antioxidant defense system. While in the case of MWCNTs, adverse effects were primarily due to altering/hampering the enzymatic antioxidant system. Data indicate the applicability of human lung cancer cells-A549 as a pre-screening tool to identify the target specific prophylactic and therapeutic potential of drugs candidate molecules against nanoparticles induced cellular damages

A new family of periplasmic-binding proteins that sense arsenic oxyanions

Arsenic contamination of drinking water affects more than 140 million people worldwide. While toxic to humans, inorganic forms of arsenic (arsenite and arsenate), can be used as energy sources for microbial respiration. AioX and its orthologues (ArxX and ArrX) represent the first members of a new sub-family of periplasmic-binding proteins that serve as the first component of a signal transduction system, that's role is to positively regulate expression of arsenic metabolism enzymes. As determined by X-ray crystallography for AioX, arsenite binding only requires subtle conformational changes in protein structure, providing insights into protein-ligand interactions. The binding pocket of all orthologues is conserved but this alone is not sufficient for oxyanion selectivity, with proteins selectively binding either arsenite or arsenate. Phylogenetic evidence, clearly demonstrates that the regulatory proteins evolved together early in prokaryotic evolution and had a separate origin from the metabolic enzymes whose expression they regulate