Moderate Exercise Attenuates Lipopolysaccharide-induced Inflammation And Associated Maternal And Fetal Morbidities In Pregnant Rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fetal growth restriction (FGR) and coagulopathies are often associated with aberrant maternal inflammation. Moderate-intensity exercise during pregnancy has been shown to increase utero-placental blood flow and to enhance fetal nutrition as well as fetal and placental growth. Furthermore, exercise is known to reduce inflammation. To evaluate the effect of moderate-intensity exercise on inflammation associated with the development of maternal coagulopathies and FGR, Wistar rats were subjected to an exercise regime before and during pregnancy. To model inflammation-induced FGR, pregnant rats were administered daily intraperitoneal injections of E. coli lipopolysaccharide (LPS) on gestational days (GD) 13.5-16.5 and sacrificed at GD 17.5. Control rats were injected with saline. Maternal hemostasis was assessed by thromboelastography. Moderate-intensity exercise prevented LPS-mediated increases in white blood cell counts measured on GD 17.5 and improved maternal hemostasis profiles. Importantly, our data reveal that exercise prevented LPS-induced FGR. Moderate-intensity exercise initiated before and maintained during pregnancy may decrease the severity of maternal and perinatal complications associated with abnormal maternal inflammation.11Canadian Bureau for International Education - Department of Foreign Affairs and International Trade (CBIE/DFAIT)Coordination for the Improvement of Higher Education Personnel-The Ministry of Education of Brazil (CAPES/MEC)FAEPEX (University of Campinas)Canadian Institutes of Health Research (CIHR) [MOP 119496]Canadian Haemophilia SocietyCIHR Doctoral Award-Frederick Banting and Charles Best Canada Graduate ScholarshipOntario Graduate ScholarshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Behavioral Therapy and Pelvic Floor Muscle Training in the Treatment of Infantile Insensible Urinary Incontinence: a Case Report

[Purpose] The purpose of this study was to evaluate the efficacy of behavioral therapy and pelvic floor muscle training in the treatment of a 5 year-old female child presenting insensible urinary incontinence (IUI) symptoms. [Subjects and Methods] Outcome measures included a voiding diary to quantify symptoms and urinary frequency, and surface electromyography was measured at the beginning and end of the treatment. Behavioral therapy included the provision of information regarding hygiene habits, voiding position, the anatomical and physiological basis of urinary incontinence, and bladder training using a voiding diary on a specified schedule. Pelvic floor muscle training was performed in different positions using sets of sustained and fast contractions. The child received training in a total of 25 sessions. [Results] The child showed improvement of symptoms according to the voiding diary and electromyographic values. [Conclusion] Behavior therapy and pelvic floor muscle training following to our protocol may have improved the symptoms of IUI in this child.25335535

Sex-specific variation in signaling pathways and gene expression patterns in human leukocytes in response to endotoxin and exercise

BACKGROUND: While exercise effects on the immune system have received increasing attention in recent years, it remains unclear to what extent gender and fluctuations in sex hormones during menstrual cycle influence immunological responses to exercise. METHODS: We investigated mRNA changes induced through exhaustive exercise (half-marathon; pre-exercise and post-exercise [30 min, 3 h, 24 h] on whole blood cultures ± lipopolysaccharide [LPS] [1 h]) with a specific focus on sex differences (men vs women in luteal phase) as an extension of our previous study. RESULTS: Inflammation related signaling pathways, TLRs, cytosolic DNA sensing and RIG-I like receptors were differentially activated between sexes in LPS-stimulated cultures. Genes differentially regulated between sexes included TNIP-1, TNIP-3, IL-6, HIVEP1, CXCL3, CCR3, IL-8, and CD69, revealing a bias towards less anti-inflammatory gene regulation in women compared to men. In addition, several genes relevant to brain function (KMO, DDIT4, VEGFA, IGF1R, IGF2R, and FGD4) showed differential activation between sexes. Some of these genes (e.g., KMO in women, DDIT4 in both sexes) potentially constitute neuroprotective mechanisms. CONCLUSIONS: These data reveal that the exercise-induced change in gene expression might be gender and menstrual cycle phase dependent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12974-016-0758-5) contains supplementary material, which is available to authorized users