The examination of protective effects of gallic acid against damage of oxidative stress during induced-experimental renal ischemia-reperfusion in experiment

Aim: In this study, probable effects of gallic acid were investigated in experimentally induced renal I/R injury in rats. Material and methods: For this purpose, each group consisted of 7 Sprague dawley male albino rats. Groups were defined as follows; Group I: control group; Group II: I/R group; Group III, IV and V: I/R+Gallic acid (50, 100 and 200 mg.kg(-1) respectively-i.p.). Left kidney was removed by nephrectomy except for Group I. I/R was induced in the other kidney. Gallic acid was given 15 mins before ischemia induction. SOD, CAT and Gpx activities were determined by electrophoresis. MDA, MPO levels were determined spectrophotometrically. Histopathological investigations were also performed in kidney tissues. BUN and Creatinine levels in serum were determined. Results: BUN, Creatinine and MDA levels were statistically significant but MPO level was not statistically significantly increased in Group II. For SOD, CAT, Gpx activities in Group II, an increase was determined with respect to Group I. Histopathological investigations revealed widespread hyperemia in glomerulus, expansion of the structure between tubules and cell disruptions in Group II. In Group V (200 mg.kg-1 gallic acid), in terms of biochemical parameters, in spite of the significant decrease in BUN, Creatinine and MDA levels; a decrease was determined in SOD, CAT and Gpx isoenzyme activities. Group V showed histologically that I/R injury had been prevented to a greater extent and appearances were close to the control. Conclusion: As a result, in terms of our study, evaluations regarding kidney functions and histopathology have shown that gallic acid has protective effects in renal I/R injury (Tab. 2, Fig. 5, Ref. 36). Text in PDF www.elis.sk

Marka Hukuku tarihi

Ankara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2017.This work is a student project of the The Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.by Emiroğlu, Kudret

Prenatal alcohol-induced neuroapoptosis in rat brain: Protection by folic acid and betaine

İstanbul Bilim Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu.Fetal alcohol syndrome (FAS) was first defined as mental retardation and growth deficits together with facial anomalies in newborns from chronic alcohol consumer mothers. All over the world, 1-2 out of every 1000 births are born with FAS. This ratio increases in populations with higher welfare. It is stated in many studies that prenatal alcohol consumption trigger apoptotic neurodegeneration

Preventive role of gallic acid on alcohol dependent and cysteine protease-mediated pancreas injury

In order to investigate an association between alcohol consumption and lysosomal cysteine protease induced pancreatic injury and preventive effect of gallic acid as dose-dependent, we determined myeloperoxidase and malondialdehyde levels, serum amylase activities and cathepsin B and L activities in the cytosolic and lysosomal fractions of pancreatic tissue in the ethanol (8 g/kg) and ethanol plus gallic acid (at different doses 50, 100 and 200 mg/kg) given rats. Absolute ethanol (8 g/kg) was given by oral gavage. Gallic acid was dissolved in the saline (2 ml/kg) and administered before 30 min the oral administration of ethanol. Pancreatic myeloperoxidase and also malondialdehyde levels and serum amylase activities were measured. Besides, histological investigations were made. Cathepsin B activities in the cytosolic fraction were decreased by gallic acid (200 mg/kg) and increased in ethanol given rats. Cytosolic/lysosomal ratio of cathepsin B and L were found to be low in the all doses of gallic acid as compared to ethanol group. Serum amylase, pancreatic myeloperoxidase activities and malondialdehyde levels in the ethanol group were higher than in the control group. These were not statistically significant for myeloperoxidase and malondialdehyde. Also, our histopathologic results indicated that ethanol administration increased pancreatic tissue injury. Gallic acid especially at 200 mg/kg improved ethanol-mediated pancreatic tissue damage.In conclusion, gallic acid treatments were decreased release of lysosomal cathepsin B and L enzymes into cytoplasmic fraction and prevented alcohol mediated pancreatic tissue injury. Preventive effect of gallic acid might be dose-dependent

Boric acid supplementation can prevent oxidative damage caused by prenatal alcohol exposure in rat cerebral cortex

İstanbul Bilim Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu.Depending on its acute or chronic usage and the dosage, alcohol has toxic effects on both mother and fetus when it is administered during pregnancy 1. Up till now, fetal alcohol spectrum disorders (FASD) has been shown to include alcohol-related birth defects (ARBD), alcohol-related neurological disorders (ARND) and fetal alcohol syndrome (FAS). Children born with FAS have some difficulties in learning, memory, attention span, communication, hearing and vision. In order to overcome this problem, new strategies should be developed to prevent the teratogenic effects of alcohol on fetus. Oxidative stress has been shown to be related to many diseases including FAS 2. Oxidative stress leads to increasing lipid peroxidation and reactive oxygen species (ROS) formation that results in malfunction of the enzymes and membrane disruption 3. Antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) have role in ROS detoxification

Effects of black hoof medicinal mushroom, phellinus linteus (Agaricomycetes), polysaccharide extract in streptozotocin-induced diabetic rats

In this article we report the healing effects of a Phellinus linteus fruiting body hot water extract (PLE) in streptozotocin (STZ)−induced diabetic rats. PLE was given before and after STZ. The preprotective, protective, and postprotective effects of PLE on STZ-induced oxidative stress were studied using biochemical (caspase 3 activity, cytosolic-to-lysosomal ratio of cathepsin B and L, DNA fragmentation levels), ordinary histological and immuno-histochemical investigation parameters. Following oral administration of PLE after STZ application, the serum glucose concentration significantly decreased up to 41.13% compared with the control group (P < 0.05). The hypoglycemic potential of the PLE was further supported by an increase of insulin secretion in the islets of Langerhans. In addition, the number of cells in Langerhans islets increased by 45.89% when PLE was given after STZ application. On the other hand, the use of PLE before oxidative stress could not prevent the onset of diabetes. This is, to our knowledge, the first study of the effect of application time of orally administered Ph. Linteus hot water extract on STZ-induced diabetes