18 research outputs found
How Dynamic Simulation Helped Mitigate Vapor Disposal System
PresentationSiemens recently completed a refinery wide pressure relief and flare analysis for two major refineries in the United States. Major deficiencies were identified on the existing flares if the relief loads calculated using steady state methods were relieved. The calculated relief loads based on the steady state method were overly conservative and resulted in relief devices and vapor disposal systems with inadequate capacities. A systematic approach to reduce the relief loads to the flare was conducted, which included performing dynamic simulation on major contributors to the flare. This systematic approach reduced the conservative assumptions and improved the predictions of the relief loads. Guidance for dynamic simulation was in line with approaches allowed per API- 521[1]. The governing case was determined and analyzed for the dynamic analysis. Validation of the results from the dynamic study helped to understand the reason behind the reduction in relief load. The study also predicted potential reduction in relief load that can be achieved prior to running dynamic simulation which is dependent on type of distillation tower (i.e. tower with conventional steam reboiler vs atmospheric towers with feed furnaces) and global release (i.e. boil up vs loss of overhead cooling). Combined credits from dynamic simulation, instrumentation and Quantitative Risk Analysis (QRA) enabled the client to understand the risk, thus helping find the most feasible and economical engineering solution to address the concern. This paper will highlight results, benefits, basis and assumptions of the detailed dynamic simulation study
Human miRNAs: an antiviral defense mechanism
Background
miRNAs are short 21-24 nt RNAs that mediate post transcriptional repression of target genes. Various reports have shown that miRNAs are capable of repressing the gene expression levels of different viruses, leading to the suggestion that miRNAs are key mediators of host-virus interaction [1]. HIV-1 is a retrovirus known to cause AIDS, one of the major diseases in humans. The nef gene of the HIV-1 has been shown to be important for virus repression of CD4+ cells and virus progression. It has also been shown earlier that patients infected with nef deleted HIV-1 do not progress from infected to diseased state for longer periods of time, resulting in the Long Term Non-Progressor phenotype [2].
Materials and methods
We computationally predicted five endogenously expressed human miRNAs to target the nef gene of HIV-1 retrovirus. On applying other stringency parameters we could focus on two of the five miRNAs viz. hsa-mir-29a and hsa-mir-29b as they were predicted to target the nef gene, at sites highly conserved amongst other clades of HIV-1 [3].
We then created reporter carrying the nef gene inserted downstream of a luciferase reporter. miRNA expression vectors were also made which would express the pri-miRNA when processed and thereby lead to high levels of the miRNA inside the cells. We then identified various cell lines for validating nef as a target for hsa-mir-29a and hsa-mir-29b.
Results and discussion
Gene reporter assays and ectopic over-expression of miRNAs conclusively showed that human cellular miRNAs hsa-mir-29a and hsa-mir-29b could bring down the nef protein levels and also affect viral replication [4]. These results would provide a better understanding of the mechanisms that could regulate the viral gene expression and human cellular antiviral defense mechanisms whereby miRNAs could serve as potential therapeutics to treat various viral diseases
Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication
protein expression and HIV-1 replicatio
MicroRNA-mediated up-regulation of an alternatively polyadenylated variant of the mouse cytoplasmic β-actin gene
Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3′-untranslated region (UTR) is not characterized and there are no experimentally determined polyadenylation (polyA) sites in actin mRNA. We have found that the cytoplasmic β-actin (Actb) gene generates two alternative transcripts terminated at tandem polyA sites. We used 3′-RACE, EST end analysis and in situ hybridization to unambiguously establish the existence of two 3′-UTRs of varying length in Actb transcript in mouse neuronal cells. Further analyses showed that these two tandem polyA sites are used in a tissue-specific manner. Although the longer 3′-UTR was expressed at a relatively lower level, it conferred higher translational efficiency to the transcript. The longer transcript harbours a conserved mmu-miR-34a/34b-5p target site. Sequence-specific anti-miRNA molecule, mutations of the miRNA target region in the 3′-UTR resulted in reduced expression. The expression was restored by a mutant miRNA complementary to the mutated target region implying that miR-34 binding to Actb 3′-UTR up-regulates target gene expression. Heterogeneity of the Actb 3′-UTR could shed light on the mechanism of miRNA-mediated regulation of messages in neuronal cells
Notch-Induced miR-708 Antagonizes Satellite Cell Migration and Maintains Quiescence
International audienceCritical features of stem cells include anchoring within a niche and activation upon injury. Notch signaling maintains skeletal muscle satellite (stem) cell quiescence by inhibiting differentiation and inducing expression of extracellular components of the niche. However, the complete spectrum of how Notch safeguards quiescence is not well understood. Here, we perform Notch ChIP-sequencing and small RNA sequencing in satellite cells and identify the Notch-induced microRNA-708, which is a mirtron that is highly expressed in quiescent cells and sharply downregulated in activated cells. We employ in vivo and ex vivo functional studies, in addition to live imaging, to show that miR-708 regulates quiescence and self-renewal by antagonizing cell migration through targeting the transcripts of the focal-adhesion-associated protein Tensin3. Therefore, this study identifies a Notch-miR708-Tensin3 axis and suggests that Notch signaling can regulate satellite cell quiescence and transition to the activation state through dynamic regulation of the migratory machinery