Depistage De L’anemie En Urgence Au Chu Campus : Validation Du Degre De Concordance Entre Les Resultats De L’hemocue® Hb 301 Et Ceux Du Sysmex Xn-1000®

Introduction: Un dépistage fiable de l’anémié dans un contexte d’urgence grâce à un hémoglobinomètre de biologie délocalisée demeure un défis en Afrique. Le but de cette étude était d’évaluer le degré de concordance des résultats d’hémoglobinémie obtenus sur l’HemoCue® Hb 301 par rapport à ceux de l’analyseur Sysmex XN-1000®. Méthodes : Il s’est agi d’une étude rétrospective et transversale descriptive réalisée du 08 Mai 2017 au 23 Février 2018 au CHU Campus du Togo. La performance du Sysmex XN-1000®  a été confirmée grâce à l’évaluation de l’exactitude et de la fidélité de mesure. La performance de l’Hemocue® Hb 301 a été évaluée au moyen d’une régression linéaire et du diagramme de Bland et Altman vis-à-vis du Sysmex XN-1000®. Les critères de performances utilisés étaient ceux du Clinical Laboratory Improvement Amendments (CLIA) et de RICOS et al.  Résultats: Le niveau d’exactitude selon CLIA du Sysmex XN-1000® était de 100%. L’équation de la droite de régression était: Y (HemoCue® Hb 301) = 0,99x (Symex XN-1000®) + 0,54 g/dl. Les valeurs médianes des 100 échantillons sur le Sysmex et l’Hemocue étaient respectivement de 12,55 g/dl et 13,00 g/dl avec un biais médian de 3,58%, qui était inférieur au biais limite de ±7,0% (CLIA). Conclusion: Le Sysmex XN-1000® fournit des résultats d’hémoglobinémie interchangeables avec ceux de l’HemoCue® Hb 301, sans altération de la capacité diagnostique pour le patient. Il reste donc performant pour un quelconque dosage d’hémoglobinémie en urgence.  Introduction: Reliable anemia screening in an emergency setting, using a Point-of-care hemoglobinometer remains a challenge in Africa. The purpose of this study was to assess the degree of agreement between hemoglobin results obtained on the HemoCue® Hb 301 compared to those of the Sysmex XN-1000® Analyzer. Methods: This was a retrospective and cross-sectional descriptive study carried out from May 08 2017 to February 23, 2018 at the Campus teaching hospital of Togo. The performance of the Sysmex XN-1000® was confirmed by evaluating the measurement accuracy and precision. The performance of Hemocue® Hb 301 was assessed using linear regression and a Bland and Altman plot against Sysmex XN-1000®. The performance criteria used were those of Clinical Laboratory Improvement Amendments (CLIA) and RICOS et al. Results: The CLIA level of accuracy of the Sysmex XN-1000® was 100%. The equation for the regression line was: Y (HemoCue® Hb 301) = 0.99x (Symex XN-1000®) + 0.54 g / dl. The median values of the 100 samples on the Sysmex and Hemocue were 12.55 g / dl and 13.00 g / dl respectively, with a median bias of 3.58%, which was less than the borderline bias of ± 7.0 % of CLIA. Conclusion: The Sysmex XN-1000® provides interchangeable hemoglobin results with those of HemoCue® Hb 301, without altering the diagnostic ability for the patient. It therefore remains effective for any emergency dosage of hemoglobinemia

Antibacterial activity of alkaloids from Sida acuta

Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various pharmacological properties. Among compounds of pharmacological interest occurring in the plant, are indoloquinoline alkaloids. The aim of the present study was to investigate the antimicrobial activity of alkaloids of S. acuta from Burkina Faso. The alkaloids had a good antimicrobial activity against the test microorganisms. In the agar-well diffusion assay, highest inhibition zone diameters were recorded with Gram-positive bacteria. The broth microdilution assay gave minimal inhibitory concentration values ranging from 16 to 400 μg/ml and minimal bactericidal concentration values ranging from 80 to up to 400 μg/ml. The gas chromatography-mass spectrometry analysis of the same alkaloids led to the identification of cryptolepine and quindoline as the major components.Keywords: Cryptolepine, quindoline, indoloquinolines, antibacterial, Sida acut

Assessment of Knowledge and Consumption Patterns of Soybean, Moringa Oleifera, Aloe Vera and Other Agro-Resources: The Case of the Elderly and Pregnant/Nursing Women in Lome, Togo

Purpose: To assess how soybean products and other agro-resources are known and consumed by some vulnerable populations in Lome (Togo). Methods: A cross sectional study was conducted from March to April 2015 among people aged 50 years and over attending two Pension Fund departments and pregnant and nursing women attending Bè Hospital in Lome. Data were collected using a pre-tested face-to-face questionnaire. Participants were surveyed about their knowledge and consumption habits of soybean, Moringa oleifera, Aloe vera products and other agro-resources. Results: A total of 847 respondents were included in the study with 426 pregnant/nursing women and 421 people aged 50 years and over. The majority of respondents were married (39.5%) and 45.3% of the elderly people were widowed. At least nine over ten respondents (96%) knew soybean products and 76.8% of them reported a lifetime consumption of soybean products. The available and most consumed agro-resources included tofu (82.7%), moringa leaves (87.2%) and soy flour (29.7%). The least consumed products were soymilk, soy yogurt and moringa juice. Respondents also reported using moringa tea and soap, as well as Aloe vera juice and toothpaste. Conclusion: Soybean products and other agricultural resources are well known and consumed by people living in Lome. Data on actual food intake is useful to public health professionals and food safety risk assessors. It is important to inform the public at large of the recognized benefits of some foods that will provide solutions to their health problems

Reliable Determination of Anaemia Prevalence, among 2175 Children Admitted to Campus University Hospital of Togo

Introduction:  Anaemia prevalence among children under 5 years old, in the world in 2011 according to WHO was at 62.8%. WHO-AFRO in 2015 stated that in most Africa countries, laboratory infrastructures and testing quality remain in budding stages. The purpose of this study was to ensure the accuracy of the haemoglobin test, for a reliable estimation of the anaemia prevalence among children with comparison of this prevalence to some previous national data.Materials and Methods: This retrospective descriptive study was performed from May 2017 to February 2018 on quality control and patient’s outcome registers, at the Campus University Hospital Laboratory. The accuracy of The Sysmex XN-1000® was evaluated by using the Clinical Laboratory Improvement Amendments criterion. The anaemia prevalence was assessed with WHO anaemia criteria.Results: The accuracy conformity rate of haemoglobin test was 100%. The prevalence of anaemia, ranging from 50.2% to 68.6%, was higher among children aged of 12 to 14. The highest prevalence of severe anaemia (22.8%) as well as moderate anaemia (29.1%) was observed among 5 to 11 years children.Conclusion: The Sysmex XN-1000® provides accurate haemoglobin test results. The anaemia prevalence in 2018 among children admitted to Campus University hospital and the previous ones are still high.&nbsp

Antibacterial activity of alkaloids from Sida acuta

Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various pharmacological properties. Among compounds of pharmacological interest occurring in the plant, are indoloquinoline alkaloids. The aim of the present study was to investigate the antimicrobial activity of alkaloids of S. acuta from Burkina Faso. The alkaloids had a good antimicrobial activity against the test microorganisms. In the agar-well diffusion assay, highest inhibition zone diameters were recorded with Gram-positive bacteria. The broth microdilution assay gave minimal inhibitory concentration values ranging from 16 to 400 μg/ml and minimal bactericidal concentration values ranging from 80 to up to 400 μg/ml. The gas chromatography-mass spectrometry analysis of the same alkaloids led to the identification of cryptolepine and quindoline as the major components