A Maternal Death due to an Unsafe Abortion in Rural Nepal - A Case Report

Background: Abortion was illegal before 2002 A.D. in Nepal and was legalized under the 11th amendment to the Country Code (Muluki Ain) in March 2002, receiving royal assent in September 2002. Nepal Government began providing comprehensive abortion care services from March 2004 after 18 months of legalization of abortion, when the government issued Safe Abortion Service Procedure. The maternal mortality ratio in Nepal is among the highest in South Asia. Unsafe abortion is defined as an induced abortion process either conducted by less skilled personnel or performed in a non-accredited facility. Though abortion has been legal in Nepal for more than 14 years now; physicians in Nepal still face problems of abortions done by less qualified personnel.Case Report: We report a fatal case of unsafe abortion in a 32 year old lady whose cause of death was ascertained as irreversible septic shock due to septic abortion.Conclusion: Prompt diagnosis and appropriate intervention might provide better outcome in these types of cases. Therefore, we stress the importance of safe abortion services provided by skilled personnel at rural areas in developing countries and of early referral in case complication arises so that the life of the patient is not endangered

Congenic Strain Analysis Reveals Genes That Are Rapidly Evolving Components of a Prezygotic Isolation Mechanism Mediating Incipient Reinforcement

Two decades ago, we developed a congenic strain of Mus musculus, called b-congenic, by replacing the androgen-binding protein Abpa27a allele in the C3H/HeJ genome with the Abpa27b allele from DBA/2J. We and other researchers used this b-congenic strain and its C3H counterpart, the a-congenic strain, to test the hypothesis that, given the choice between signals from two strains with different a27 alleles on the same genetic background, test subjects would prefer the homosubspecific one. It was our purpose in undertaking this study to characterize the segment transferred from DBA to the C3H background in producing the b-congenic strain on which a role for ABPA27 in behavior has been predicated. We determined the size of the chromosome 7 segment transferred from DBA and the genes it contains that might influence preference. We found that the “functional" DBA segment is about 1% the size of the mouse haploid genome and contains at least 29 genes expressed in salivary glands, however, only three of these encode proteins identified in the mouse salivary proteome. At least two of the three genes Abpa27, Abpbg26 and Abpbg27 encoding the subunits of androgen-binding protein ABP dimers evolved under positive selection and the third one may have also. In the sense that they are subunits of the same two functional entities, the ABP dimers, we propose that their evolutionary histories might not be independent of each other

Genetic variation and possible origins of weedy rice found in California.

Control of weeds in cultivated crops is a pivotal component in successful crop production allowing higher yield and higher quality. In rice-growing regions worldwide, weedy rice (Oryza sativa f. spontanea Rosh.) is a weed related to cultivated rice which infests rice fields. With populations across the globe evolving a suite of phenotypic traits characteristic of weeds and of cultivated rice, varying hypotheses exist on the origin of weedy rice. Here, we investigated the genetic diversity and possible origin of weedy rice in California using 98 simple sequence repeat (SSR) markers and an Rc gene-specific marker. By employing phylogenetic clustering analysis, we show that four to five genetically distinct biotypes of weedy rice exist in California. Analysis of population structure and genetic distance among individuals reveals diverse evolutionary origins of California weedy rice biotypes, with ancestry derived from indica, aus, and japonica cultivated rice as well as possible contributions from weedy rice from the southern United States and wild rice. Because this diverse parentage primarily consists of weedy, wild, and cultivated rice not found in California, most existing weedy rice biotypes likely originated outside California

Phyllochron Development in Cool-Season Forage Grasses

The purpose of this study was to evaluate the relationship between grass leaf insertion rate and accumulated growing degree-days, and determine the phyllochron for five perennial forage grass species and two cultivars of each species. Species field seeded in solid stands were crested wheatgrass [Agropyron desertorum (Fisch. ex. Link) Schult.], intermediate wheatgrass, [Thinopyrum intermedium Barkw. & Dewey:Syn:A. intermedium (Host) Brauv], western wheatgrass [Pascopyrum smithii (Rybd) L ve], green needlegrass (Stipa viridula Trin.), and smooth bromegrass (Bromus inermis Leyss). Species phyllochron differences ranged from 77 GDD for Mandan 404 smooth bromegrass to 114 GDD for Lodorm green needlegrass. Phyllochron differences were greater among species than cultivars within species which suggests a single equation is adequate for predicting species phyllochron for determining grazing readiness

Dynamic classification using case-specific training cohorts outperforms static gene expression signatures in breast cancer

The molecular diversity of breast cancer makes it impossible to identify prognostic markers that are applicable to all breast cancers. To overcome limitations of previous multigene prognostic classifiers, we propose a new dynamic predictor: instead of using a single universal training cohort and an identical list of informative genes to predict the prognosis of new cases, a case-specific predictor is developed for each test case. Gene expression data from 3,534 breast cancers with clinical annotation including relapse-free survival is analyzed. For each test case, we select a case-specific training subset including only molecularly similar cases and a case-specific predictor is generated. This method yields different training sets and different predictors for each new patient. The model performance was assessed in leave-one-out validation and also in 325 independent cases. Prognostic discrimination was high for all cases (n = 3,534, HR = 3.68, p = 1.67 E-56). The dynamic predictor showed higher overall accuracy (0.68) than genomic surrogates for Oncotype DX (0.64), Genomic Grade Index (0.61) or MammaPrint (0.47). The dynamic predictor was also effective in triple-negative cancers (n = 427, HR = 3.08, p = 0.0093) where the above classifiers all failed. Validation in independent patients yielded similar classification power (HR = 3.57). The dynamic classifier is available online at http://www.recurrenceonline.com/?q=Re_training. In summary, we developed a new method to make personalized prognostic prediction using case-specific training cohorts. The dynamic predictors outperform static models developed from single historical training cohorts and they also predict well in triple-negative cancers

Bacterial Spore Detection by [Tb^(3+)(macrocycle)(dipicolinate)] Luminescence

Dipicolinic acid (DPA) is a unique constituent of bacterial spores, a dormant form of Bacillus and Clostridium, which can be detected using DPA-triggered Tb^(3+) luminescence. [Tb(DO2A)]^+ improves the sensitivity of bacterial spore detection over Tb^(3+)(aq) owing to the exclusion of coordinated water molecules and represents the first step toward construction of a DPA receptor site with enhanced binding selectivity. The title ternary [Tb(DO2A)(DPA)]- complex was structurally characterized and features two DO2A-DPA interligand hydrogen interactions that stabilize the complex

Decorating Metal Oxide Surfaces with Fluorescent Chlorosulfonated Corroles

We have prepared 2,17-bis(chlorosulfonyl)-5,10,15-tris(pentafluorophenyl)corrole (1), 2,17-bis(chlorosulfonyl)-5,10,15-tris(pentafluorophenyl)corrolatoaluminum(III) (1-Al), and 2,17-bis(chlorosulfonyl)-5,10,15-tris(pentafluorophenyl)corrolatogallium(III) (1-Ga). The metal complexes 1-Al and 1-Ga were isolated and characterized by electronic absorption and NMR spectroscopies, as well as by mass spectrometry. Relative emission quantum yields for 1, 1-Al, and 1-Ga, determined in toluene, are 0.094, 0.127, and 0.099, respectively. Reactions between 1, 1-Al, and 1-Ga and TiO2 nanoparticles (NPs) result in corrole–TiO_2 NP conjugates. The functionalized NP surfaces were investigated by solid-state Fourier transform infrared and X-ray photoelectron spectroscopies and by confocal fluorescence imaging. The fluorescence images for 1-Al–TiO_2 and 1-Ga–TiO_2 suggest a promising application of these NP conjugates as contrast agents for noninvasive optical imaging

Home sweet home: a critical comment on Saunders and Williams

While accepting the need for research which focuses on the social meaning of the home, this article takes issue with some of Saunders’ and Williams’ formulations for a research programme — in particular, the emphasis given to physical and design features of the home at the expense of an understanding of more fundamental gender and generational relations within the home. It attempts to uncover the assumptions lying behind those formulations, to clarify some of the conceptual confusions, and to point out some of the serious theoretical difficulties which such formulations have to resolve. It argues that theoretical advance in this area does not have to depend upon the adoption of a Weberian perspective, but must be situated within a broader theory of the production and maintenance of ideology, and this theory must be explicitly linked with theories of power and kinship. In particular, it is emphasised that power relations within the home, associated mainly with gender and age differences, need to be investigated in greater depth if the social significance of the home is to be properly understood

A Candidate Subspecies Discrimination System Involving a Vomeronasal Receptor Gene with Different Alleles Fixed in M. m. domesticus and M. m. musculus

Assortative mating, a potentially efficient prezygotic reproductive barrier, may prevent loss of genetic potential by avoiding the production of unfit hybrids (i.e., because of hybrid infertility or hybrid breakdown) that occur at regions of secondary contact between incipient species. In the case of the mouse hybrid zone, where two subspecies of Mus musculus (M. m. domesticus and M. m. musculus) meet and exchange genes to a limited extent, assortative mating requires a means of subspecies recognition. We based the work reported here on the hypothesis that, if there is a pheromone sufficiently diverged between M. m. domesticus and M. m. musculus to mediate subspecies recognition, then that process must also require a specific receptor(s), also sufficiently diverged between the subspecies, to receive the signal and elicit an assortative mating response. We studied the mouse V1R genes, which encode a large family of receptors in the vomeronasal organ (VNO), by screening Perlegen SNP data and identified one, Vmn1r67, with 24 fixed SNP differences most of which (15/24) are nonsynonymous nucleotide substitutions between M. m. domesticus and M. m. musculus. We observed substantial linkage disequilibrium (LD) between Vmn1r67 and Abpa27, a mouse salivary androgen-binding protein gene that encodes a proteinaceous pheromone (ABP) capable of mediating assortative mating, perhaps in conjunction with its bound small lipophilic ligand. The LD we observed is likely a case of association rather than residual physical linkage from a very recent selective sweep, because an intervening gene, Vmn1r71, shows significant intra(sub)specific polymorphism but no inter(sub)specific divergence in its nucleotide sequence. We discuss alternative explanations of these observations, for example that Abpa27 and Vmn1r67 are coevolving as signal and receptor to reinforce subspecies hybridization barriers or that the unusually divergent Vmn1r67 allele was not a product of fast positive selection, but was derived from an introgressed allele, possibly from Mus spretus

Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals

We describe a scanning fluorescence lifetime imaging (SFLIM) system that provides a large field of view (LFOV), using a femtosecond (fs) pulsed laser, for multi-mode optical imaging of small animals. Fluorescence lifetime imaging (FLIM) can be a useful optical method to distinguish between fluorophores inside small animals. However, difficulty arises when LFOV is required in FLIM using a fs pulsed laser for the excitation of the fluorophores at low wavelengths (<500nm), primarily because the field of view of the pulsed blue excitation light generated from the second harmonic of the fs pulsed light is limited to about a centimeter in diameter due to the severe scattering and absorption of the light inside tissues. Here, we choose a scanning method in order to acquire a FLIM image with LFOV as one alternative. In the SFLIM system, we used a conventional cooled CCD camera coupled to an ultra-fast time-gated intensifier, a tunable femtosecond laser for the excitation of fluorophores, and an x-y moving stage for scanning. Images acquired through scanning were combined into a single image and then this reconstructed image was compared with images obtained by spectral imaging. The resulting SFLIM system is promising as an alternative method for the FLIM imaging of small animals, containing fluorophores exited by blue light, for LFOV applications such as whole animal imaging