Factors Affecting Sustainability Of Wetland Agriculture Within Lake Victoria Basin In Uganda

In recent years, the high rate of conversion of wetlands for agriculture has raised environmental concerns in Uganda. A study was therefore conducted to identify issues that need to be addressed if communities are to continue deriving livelihoods from wetland agriculture, without causing stress to the wetlands of Lake Victoria basin. This was done by conducting household surveys and focus group discussions in two of the districts that border Lake Victoria. Wetland agriculture was found to be a significant source of livelihoods for resource poor communities, contributing over 60% of the household income. Farming was mainly done at a subsistence level. The study found that socio economic factors, poor farming practices and weak policies were contributing to the rapid conversion of wetlands. Concerted efforts need to be made by government institutions to sensitize communities on conservation of wetlands. Some of the policies need to be harmonized to avoid contradictions. Alternative sources of livelihoods must be created to ensure socio economic benefits and environmental conservation. Keywords: Wetland agriculture; Sustainability; Livelihoods; Uganda.Journal of Agriculture and Social Research Vol. 8 (1) 2008: pp. 78-8

Phytochemicals Present in Engleromyces goetzei and Antimicrobial Activity Against Phytopathogenic Bacteria

Interest in the extraction of bioactive compounds from natural sources has increased in recent years due to the potential applications of these compounds in food, chemical, and pharmaceutical industries. The methanolic extract of the fungus Engleromyces goetzei was investigated for the presence of phytochemicals and the antibacterial properties. The study shows that the extract has flavonoids, coumarins, saponins, terpenoids and steroids. Engleromyces goetzei has potential as a source of useful antibacterial compounds active against phytopathogenic bacteria. Disk diffusion assay was used to analyze the susceptibility of an organism which help in identifying the proper antibacterial biopesticide. In this experiment, this was done by placing the antimicrobial disks impregnated with E. goetzei on the NA plates swabbed with Xanthomonas campestris, Pseudomonas sryingae pv phaseolicola, Erwinia carotovora and Xanthomonas axonopodis pv. phaseoli. After twenty-four hours of incubation, there were areas around the antimicrobial disks with no bacterial growth. The MIC of E. goetzei against X. axonopodis pv. phaseoli, Ps. syringae pv phaseolicola and X. campestris was found to be 1mg/ml while that of E. carotovora was 10mg/ml. Keywords: Phytochemicals, Engleromyces goetzei, X. axonopodis pv. phaseoli, E. carotovora, Ps. syringae pv phaseolicola, X. campestri

Antinociceptive potentiation of pethidine (demerol) by clomipramine in the late phase of formalin test in mice

Background: Pethidine, an opioid analgesic is used for pain management. Clomipramine a tricyclic antidepressant primarily used for mood management is also used to treat pain. The objective of this study was to investigate the potentiation of the analgesic effects of sub-threshold dose of pethidine by a tricyclic antidepressant, clomipramine. Methods: The antinociceptive activities of clomipramine and pethidine alone and in combination were investigated in Swiss albino mice using the formalin test. Normal saline was employed as the control. Ten animals were used in each experiment. Results: Pethidine 5mg / kg failed to cause any significant effect while the 6.25, 7.5, 8.75 and 10.0mg /kg showed highly significant antinociceptive effect (p< 0.01) compared to the controls in the late phase of formalin test. Clomipramine 0.5 mg / kg did not show any significant effect while 0.75 mg / kg caused a significant effect (p< 0.05) while 1.00 and 1.25mg /kg caused a very highly significant antinociceptive effect (p< 0.001) in the late phase of formalin test compared to the vehicle treated animals. The combination of pethidine 5mg / kg and clomipramine 0.75mg / kg caused a highly significant antinociceptive effect (P<0.01) in the late phase of formalin test. Conclusion: This study demonstrates a marked reduction in the time spent in pain behaviour produced by the combination of low dose pethidine and clomipramine in the late phase of formalin test. The findings demonstrate the potentiation of a narcotic analgesic by a tricyclic antidepressant. Pan African Medical Journal 2012 12:2

Effect of Inoculation with Mycorrhizae on Growth Parameters of Dombeya torrida, Leucaena leucocephala and Tephrosia vogelii

The present work evaluated the impact of arbuscular mycorrhizal (AM) fungus on growth. Three species of trees, Tephrosia vogelii, Leucaena leucocephala and Dombeya torrida were treated with fragments of arbuscular mycorrhizae and grown in greenhouse. The aim of the present work was to determine whether arbuscular mycorrhizal symbiotic association with these tree species would enhance their growth. Data on height, stem diameter and number of leaves were taken after every two weeks for a period of three months. Mycorrhizal plants demonstrated higher vigor with Tephrosia vogelii giving a mean height of 57.50 cm, 0.50 cm diameter and 9.33 number of leaves which were significantly different from the control plants at p ≤ 0.05 according to least significant difference (LSD). The biomass was also taken at the end of 90 days of growth and the same trend was exhibited. Mycorrhizal plants also showed root nodules with mycorrhizal arbuscles. This work contributes to our understanding of the effects of AM symbiosis on the accumulation of certain micronutrients in valuable woody tree species. Keywords: arbuscular mycorrhizae, Tephrosia vogelii, Leucaena leucocephala, plant growt

Determinants of Women Access and the Extent of Access to Land Among Small-Scale Farmers in Machakos County, Kenya: A Double-Hurdle Approach

The aim of this paper is to evaluate the determinants of women access and the extent of access to land. The study used cross-sectional data from 384 randomly selected small-scale women farmers from Machakos County, Kenya. Using double hurdle (DH) model, we found that marital status, household size, the value of productive assets, credit borrowed, extension contacts, farmers’ groups and social influence from family members had a positive and significant influence on women access to land. However, the spousal age gap, market distance and social influence from friends/peers had a negative influence on the probability of women having access to land. To improve women access to land, the findings imply that women need to be motivated to join and participate in farmers’ groups through which they can gain access to extension information and credit. In addition, women farmers should be sensitized on the need to invest in farm productive assets. Importantly, the government can aid in improving crucial infrastructures and promoting girl-child education since education empowers women and inhibit early marriages. Keywords: Women, small-scale farmers, access to land, double hurdle model, Kenya DOI: 10.7176/JESD/10-7-04 Publication date: April 30th 201

Biocontrol of Green Mould Disease of Oyster Mushroom (Pleurotus ostreatus) using Bacillus amyloliquefaciens

The occurrence of Trichoderma harzianum and T. asperellum in cultivation of oyster mushroom (Pleurotus ostreatus) frequently results in serious crop losses and considerable inhibition of growth of mycelium and fruiting bodies of oyster mushroom thus lowering the yield substantially. Bacillus amyloliquefaciens strain isolated from groundnuts proved very effective in antagonizing the oyster mushroom pathogenic T. harzianum and T. asperellum without having a negative effect on P. ostreatus mycelia. The Bacillus amyloliquefaciens was found to produce diffusible and volatile organic compounds. This strain is a potential biocontrol candidate, in addition to the lack of antagonistic activity towards P. ostreatus mycelia. The present study, hence, provides a potential biocontrol agent for Trichoderma green mould. However, field studies of this isolate as substrate inoculant in oyster mushroom are required in order to establish its actual performance. Keywords: Bacillus amyloliquefaciens, Green mould, Mushroom, Biocontro

Treatment of shigella infections: why sulfamethoxazole-trimethoprim, tetracyclines and ampicillin should no longer be used

Background: Bloody diarrhoea results in high morbidity and mortality especially in developing countries with shigellosis being the main cause of acute bloody diarrhoea. The use of appropriate antimicrobial agents in the treatment of acute diarrheal disease shortens the duration of illness and bacterial shedding leading to a reduction in morbidity and mortality. Treatment options for many infections are becoming limited due to globally emerging antibiotic resistance. Globally, resistance of shigella species to trimethoprim-sulfamethoxazole (TMP-SMX), tetracyclines and ampicillin has been reported with subsequent recommendations of not using these antimicrobial drugs for empirical therapy of acute bloody diarrhoea.Objective: To establish the antimicrobial susceptibility patterns and antimicrobial drug use for treatment of shigella species in patients with acute bloody diarrhoea.Design: A hospital based case control study.Setting: Six health facilities, three in Kilifi County and three in Nairobi County.Subject: A total of 284 stool specimens were collected from patients who fitted the standard cases definition for acute bloody diarrhoea.Results: Eighty (28.2%) bacterial isolates were recovered from 284 stool samples collected from cases presenting with acute bloody diarrhoea of which 67 (83.8%) were Shigella species, nine (11.3%) were Enteroinvassive Escherichia coli isolates, three (3.8%) were Salmonella Typhi and one (1.3%) were Yersinia enterocolitica. Shigella isolates had high resistance to sulfamethoxazole-trimethoprim (97%), tetracycline (83.6%) ampicillin (58.2%) and chloramphenicol (20.9%). The isolates showed low resistance to nalidixic (4.5%) and ciprofloxacin (3.0%) while there was no resistance to ceftriaxone. The most common multidrug resistance pattern detected in Shigella strains combined sulfamethoxazole-trimethoprim, amoxicillin/ampicillin and tetracyclines.Antibiotic prescriptions were given to 243(85.6%) of the patients presenting with acute bloody diarrhoea. Among these, 94 (38.7%) were given prescriptions for ciprofloxacin, 53 (21.8%) for sulfamethaxazole-trimethiprin and 36(14.8%) for Tetracyclines. Chloramphenicol, amoxicillin/ampicillin, nalidixic acid and ceftriaxone were prescribed to 10.7 %, 3.7%, 2.9% and 0.4% of the patients respectively. A total of 123 (51%) received antibiotics which were ranked to have high resistance (sulfamethoxazole-trimethoprim, tetracyclines ampicillin and chloramphenicol).Conclusion: The high rates of antimicrobial resistance among the commonly prescribed antimicrobials such as sulfamethoxazole-trimethoprim, tetracycline, ampicillin and chloramphenicol is of major concern. Despite recommendations discouraging the empirical use of sulfamethoxazole-trimethoprim, tetracycline, ampicillin and chloramphenicol for treatment of acute bloody diarrhoea, more than half of the patients with acute bloody diarrhoea were still treated with these antibiotics.There is need to train health care workers on the proper management of acute bloody diarrhoea and the importance of adhering to the clinical guidelines

Synthesis, solid-state structure, and electrochemical properties of thienodipyrimidine-2,4,5,7-tetra(thi)ones

Thienodipyrimidine-2,4,5,7-tetra(thi)ones were prepared by one-pot photocyclization from barbituric acid derivatives. The structures of these tricyclic molecules with multiple (thio)carbonyl groups were determined by NMR and single-crystal X-ray diffraction analysis, and the electrochemical properties were studied by cyclic voltammetry and DFT calculations. The solid-state structures of these molecules feature dipolar C=S (or C=O), chalcogen-bonding, and π-stacking interactions. The presence of two adjacent thiocarbonyl groups allows for a two-center three-electron (2c/3e) interaction upon oxidation, significantly lowering the oxidation potential

Triplet-forming thionated donor-acceptor chromophores for electrochemically amphoteric photosensitization

Thiocarbonylation has been shown to be a promising tool for developing heavy-atom-free triplet photosensitizers from donor−acceptor chromophores. It was demonstrated that triplet-state formation from the thiocarbonyls is independent of the donor−acceptor interactions and that the thionated chromophores can be utilized as amphoteric photoredox catalysts that have comparable catalytic activities to common inorganic photosensitizers

Differential Plasmodium falciparum surface antigen expression among children with Malarial Retinopathy

Retinopathy provides a window into the underlying pathology of life-threatening malarial coma (“cerebral malaria”), allowing differentiation between 1) coma caused by sequestration of Plasmodium falciparum-infected erythrocytes in the brain and 2) coma with other underlying causes. Parasite sequestration in the brain is mediated by PfEMP1; a diverse parasite antigen that is inserted into the surface of infected erythrocytes and adheres to various host receptors. PfEMP1 sub-groups called “DC8” and “DC13” have been proposed to cause brain pathology through interactions with endothelial protein C receptor. To test this we profiled PfEMP1 gene expression in parasites from children with clinically defined cerebral malaria, who either had or did not have accompanying retinopathy. We found no evidence for an elevation of DC8 or DC13 PfEMP1 expression in children with retinopathy. However, the proportional expression of a broad subgroup of PfEMP1 called “group A” was elevated in retinopathy patients suggesting that these variants may play a role in the pathology of cerebral malaria. Interventions targeting group A PfEMP1 may be effective at reducing brain pathology