41 research outputs found
Alguns dados sobre a Fauna entomológica da ilha das Flores - Açores
IV Expedição CientÃfica do Departamento de Biologia - Flores 1989Com este trabalho, realizado em Julho de 1989 nas Flores - a ilha mais ocidental
do Arquipélago dos Açores -, acrescentaram-se onze espécies de Lepidópteros à lista referenciada para aquela ilha, pertencendo uma à famÃlia Lycaenidae (Lampides boeticus L.), oito a familia Noctuidae (Agrotis ipsilon HFN., Brotolomia meticulosa L., Chrysodeixis chalcites ESPER., Heliothis armigera HBN., Noctua atlantica WARREN, Noctua pronuba L., Peridroma saucia HBN., Sesamia nonagrioides LEF.), uma à famÃlia Nymphalidae (Vanessa atalanta L.) e uma a famÃlia Pyralidae (Glyphodes unionalis HBN.). Entre os demais insectos, foram identificadas cerca de duas dezenas e meia de espécies, distribuÃdas pelas Ordens Dermaptera, Orthoptera, Dictyoptera, Heteroptera, Homoptera, Coleoptera, Neuroptera, Diptera, Hymenoptera e Collembola. Salienta-se ainda a importância, do ponto de vista agronómico, das pragas Mythimna unipuncta (HAWORTH) e Xestia c-nigrun L. naquela ilha.RÉSUMÉ: Avec ce travail, réalisé en Juillet 1989 a Flores - l'île plus occidental de l'archipel des Açores, onze espèces de Lépidoptères ont été ajoutées à la liste des espèces connus pour cette île, dont une appartient a la famille Lycaenidae (Lampides boelicus L.), huit à la famille Noctuidae (Agrotis ipsilon HFN., Brotolomia meticulosa L. Chrysodeicis chalcites ESPER., Heliothis armigera HBN., Noctua atlantica WARREN, Noctua pronuba L., Peridroma saucia HBN., Sesamia nonagrioides LEF.), une à la famille Nymphalidae (Vanessa atalanta L.) et une à la famille Pyralidae (Glyphodes unionalis HBN.). Parmi les autres insects ont été identifiés environ deux dizaines et demie d'espèces, lesquelles sont réparties par les Ordres Dermaptera, Orthoptera, Dictyoptera, Heteroptera, Homoptera, Coleoptera, Neuroptera, Diptera, Hymenoptera et Collembola. On remarque I'importance, du point de vue agronomique, des ravageurs Mythimna unipuncra (HAWORTH) et Xestia c-nigrum L. dans cette île
Subsets of NKT cells from CVID patients.
<p>(A) Percentage of CD4 marker in NKT cells (left) (p = 0.0055). (B) Absolute number of CD4 marker in NKT cells (middle). (C) Representative flow cytometry dot plot of CD4 marker (right). (D) Percentage of CD8 marker in NKT cells (left) (p = 0.011). (E) Absolute number of CD8 marker in NKT cells (middle) (p = 0.002). (F) Representative flow cytometry dot plot of CD8 marker (right). (G) Percentage of CD161 marker in NKT cells (left). (H) Absolute number of CD161 marker in NKT cells (middle). (I) Representative flow cytometry dot plot of CD161 marker (right).</p
Demographic, clinical and laboratory characteristics of control and CVID patient groups.
<p>IQR: interquartile range.</p
Subsets of NKT cells.
<p>(A) Percentage of CD161, CD8 and CD4 markers in NKT cells (p = 0.0145), (B) Percentage of CD4 and CD161 marker in NKT cells (p = 0.001). (C) Percentage of CD8 and CD161 markers in NKT cells (p = 0.0004).</p
Percentage of activation, chemokine receptors in NKT cells.
<p>(A) Percentage of chemokine receptor CCR5 in NKT cells gate (Vα24+Vβ11) in representative healthy subject and CVID patient (p<0.0001). (B) Percentage of chemokine receptors CXCR6, CCR5 and CD69 marker in NKT cells (p<0.001). (C) Percentage of chemokine receptor CCR5 and CD69 marker in NKT cells (p<0.001). Comparisons among groups were carried out using the Mann-Whitney non-parametric test.</p
Expression of NKT cells in peripheral blood.
<p>(A) Representative flow cytometric analyses on PBMC, lymphocytes, CD3+ T cells and Vα24+Vβ11+ for NKT cells. (B) Fluorescence minus one (FMO) was used for gate strategy for CXCR6, CCR5 and CD69 in NKT cells. (C) Representative flow cytometric analyses on NKT cells in CVID patients. Comparisons among groups were carried out using the Mann-Whitney non-parametric test.</p
Clinical and laboratory characteristics of studied subjects.
<p>IQR: Inter -quartile range.</p
Flow cytometric panels showing the gating strategy from healthy and CVID patients.
<p>(A) The gate is set around the population of lymphocytes; (B) CD3 T cells that were evaluated for (C), FOXP3, CD25; (D) Side scatter and CD3<sup>+</sup>FOXP3<sup>+</sup>CD25<sup>high</sup>CD127<sup>low</sup>. (E) The gate is set around the population of FOXP3+CD25<sup>high</sup>CD127<sup>low</sup>CD4<sup>+</sup> T cells. Fluorescence minus one was used to define the gate used. Arrows indicate the gated population subsequently analyzed.</p
Comparison of Treg cell subsets between healthy controls (CTL) and CVID patients.
<p>In CVID group there was a significant lower percentage of FOXP3<sup>+</sup>CD25<sup>high</sup>CD127<sup>low</sup>CD4<sup>+</sup> T cell (<i>P</i> = 0.0034).</p
Comparisons of CD8<sup>+</sup>CD38<sup>+</sup> (A), CD8<sup>+</sup>HLA-DR<sup>+</sup> (B) and CD8<sup>+</sup>CD38<sup>+</sup>CCR5<sup>+</sup> (C) T cell percentages in PBMC in healthy controls (CTL) and CVID patients showing significant difference in the activation.
<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006269#pone-0006269-g001" target="_blank">Figure 1A</a>-, <i>P</i> = 0.002; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006269#pone-0006269-g001" target="_blank">Figure 1B</a>-, <i>P</i> = 0.008; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006269#pone-0006269-g001" target="_blank">Figure 1C, <i>P</i></a> = 0.021.</p