Animal Tracks Water Action Pack

This guide contains: Discovery, Awareness, Action and Appendices

Improved cache performance in Monte Carlo transport calculations using energy banding

We present an energy banding algorithm for Monte Carlo (MC) neutral particle transport simulations which depend on large cross section lookup tables. In MC codes, read-only cross section data tables are accessed frequently, exhibit poor locality, and are typically too much large to fit in fast memory. Thus, performance is often limited by long latencies to RAM, or by off-node communication latencies when the data footprint is very large and must be decomposed on a distributed memory machine. The proposed energy banding algorithm allows maximal temporal reuse of data in band sizes that can flexibly accommodate different architectural features. The energy banding algorithm is general and has a number of benefits compared to the traditional approach. In the present analysis we explore its potential to achieve improvements in time-to-solution on modern cache-based architectures.United States. Department of Energy. Office of Science (Contract DE-AC02-06CH11357

Infection of liver sinusoidal endothelial cells with Muromegalovirus muridbeta1 involves binding to neuropilin-1 and is dynamin-dependent

Liver sinusoidal endothelial cells (LSEC) are scavenger cells with a remarkably high capacity for clearance of several blood-borne macromolecules and nanoparticles, including some viruses. Endocytosis in LSEC is mainly via the clathrin-coated pit mediated route, which is dynamin-dependent. LSEC can also be a site of infection and latency of betaherpesvirus, but mode of virus entry into these cells has not yet been described. In this study we have investigated the role of dynamin in the early stage of muromegalovirus muridbeta1 (MuHV-1, murid betaherpesvirus 1, murine cytomegalovirus) infection in mouse LSECs. LSEC cultures were freshly prepared from C57Bl/6JRj mouse liver. We first examined dose- and time-dependent effects of two dynamin-inhibitors, dynasore and MitMAB, on cell viability, morphology, and endocytosis of model ligands via different LSEC scavenger receptors to establish a protocol for dynamin-inhibition studies in these primary cells. LSECs were challenged with MuHV-1 (MOI 0.2) ± dynamin inhibitors for 1h, then without inhibitors and virus for 11h, and nuclear expression of MuHV-1 immediate early antigen (IE1) measured by immune fluorescence. MuHV-1 efficiently infected LSECs in vitro. Infection was significantly and independently inhibited by dynasore and MitMAB, which block dynamin function via different mechanisms, suggesting that initial steps of MuHV-1 infection is dynamin-dependent in LSECs. Infection was also reduced in the presence of monensin which inhibits acidification of endosomes. Furthermore, competitive binding studies with a neuropilin-1 antibody blocked LSEC infection. This suggests that MuHV-1 infection in mouse LSECs involves virus binding to neuropilin-1 and occurs via endocytosis

Manual de procesos administrativos : Facultad de Ciencias Políticas y Sociales : 2019

El presente Manual de procesos administrativos de la Facultad de Ciencias Políticas y Sociales (FCPyS- UNCuyo) constituye una sistematización del relevamiento realizados en el marco de la materia optativa/electiva de Taller de Relevamiento y Documentación de Procesos Administrativos (Res. 100/19-CD), donde participaron estudiantes de la Licenciatura de Ciencia Política y Administración Pública, de la Licenciatura de Trabajo Social, de la Licenciatura de Sociología y de la Licenciatura de Comunicación Social durante el ciclo lectivo 2019. Las actividades de relevamiento, análisis, documentación y descripción de los procesos estratégicos, sustantivos y de apoyo desarrolladas por las y los estudiantes se efectuaron mediante la aplicación de un enfoque metodológico pre-definido, utilizando fuentes primarias (entrevistas a través de la aplicación de fichas de relevamiento, información del portal oficial de la facultad) y fuentes secundarias (normativa vigente, documentos oficiales) para realizar el presente trabajo de sistematización

On the computation of interstellar extinction in photoionized nebulae

Ueta & Otsuka (2021) proposed a method, named as the "Proper Plasma Analysis Practice", to analyze spectroscopic data of ionized nebulae. The method is based on a coherent and simultaneous determination of the reddening correction and physical conditions in the nebulae. The same authors (Ueta & Otsuka 2022, UO22) reanalyzed the results of Galera-Rosillo et al. (2022, GR22) on nine of the brightest planetary nebulae in M31. They claim that, if standard values of the physical conditions are used to compute the extinction instead of their proposed method, extinction correction is underestimated by more than 50% and hence, ionic and elemental abundance determinations, especially the N/O ratio, are incorrect. Several tests were performed to assess the accuracy of the results of GR22, when determining: i) the extinction coefficient, ii) the electron temperature and density, and iii) the ionic abundances. In the latter case, N+ /H+ ionic abundance was recalculated using both H_alpha and H_beta as the reference H I emissivity. The analysis shows that the errors introduced by adopting standard values of the plasma conditions by GR22 are small, within their quoted uncertainties. On the other hand, the interstellar extinction in UO22 is found to be overestimated for five of the nine nebulae considered. This propagates into their analysis of the properties of the nebulae and their progenitors. The python notebook used to generate all the results presented in this paper are of public access on a Github repository. The results from GR22 are proven valid and the conclusions of the paper hold firmly. Although the PPAP is, in principle, a recommended practice, we insist that it is equally important to critically assess which H I lines are to be included in the determination of the interstellar extinction coefficient, and to assert that physical results are obtained for the undereddened line ratios.Comment: Accepted for publication in A&A Lette

Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIB, yet maintain a preserved fenestration the Glmp gt/gt mouse model of slowly progressing liver fibrosis

Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmpgt/gt mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born normal, but soon develops chronic, mild hepatocyte injury, leading to slowly progressing periportal liver fibrosis, and splenomegaly. This study examined how LSEC scavenger function and morphology are affected in the Glmpgt/gt model. FITC-labelled formaldehyde-treated serum albumin (FITC-FSA), a model ligand for LSEC scavenger receptors was administered intravenously into Glmpgt/gt mice, aged 4 months (peak of liver inflammation), 9–10 month, and age-matched Glmpwt/wt mice. Organs were harvested for light and electron microscopy, quantitative image analysis of ligand uptake, collagen accumulation, LSEC ultrastructure, and endocytosis receptor expression (also examined by qPCR and western blot). In both age groups, the Glmpgt/gt mice showed multifocal liver injury and fibrosis. The uptake of FITC-FSA in LSECs was significantly reduced in Glmpgt/gt compared to wild-type mice. Expression of LSEC receptors stabilin-1 (Stab1), and mannose receptor (Mcr1) was almost similar in liver of Glmpgt/gt mice and agematched controls. At the same time, immunostaining revealed differences in the stabilin-1 expression pattern in sinusoids and accumulation of stabilin-1-positive macrophages in Glmpgt/gt liver. FcγRIIb (Fcgr2b), which mediates LSEC endocytosis of soluble immune complexes was widely and significantly downregulated in Glmpgt/gt liver. Despite increased collagen in space of Disse, LSECs of Glmpgt/gt mice showed well-preserved fenestrae organized in sieve plates but the frequency of holes >400 nm in diameter was increased, especially in areas with hepatocyte damage. In both genotypes, FITC-FSA also distributed to endothelial cells of spleen and bone marrow sinusoids, suggesting that these locations may function as possible compensatory sites of clearance of blood-borne scavenger receptor ligands in liver fibrosis

Development and implementation of an Internet-based survivorship care program for cancer survivors treated with hematopoietic stem cell transplantation.

The Internet provides a widely accessible modality for meeting survivorship care needs of cancer survivors. In this paper, we describe the development and implementation of an Internet site designed as a base from which to conduct a randomized controlled trial to meet psycho-educational needs of hematopoietic stem cell transplantation (HSCT) survivors

Processamento de conservas de tomates orgânicos desidratados: Uma alternativa para agricultura familiar

A demanda por alimentos mais saudáveis têm crescido rapidamente e a desidratação aplicada aos alimentos orgânicos têm se apresentado como uma ferramenta tecnológica importante para o desenvolvimento de produtos mantendo a integridade orgânica. O tomate que apresenta produção e consumo mais difundidos no mundo, quer in natura ou processado, é altamente perecível, com perdas estimadas entre 25 a 50% da produção. Sendo assim, esta pesquisa objetivou a realização do processamento de tomates orgânicos desidratados em conservas obtidos a partir de dois diferentes equipamentos (desidratador e forno doméstico), com utilização de padrões para a pequena agroindústria e artesanais. Utilizaram-se três variedades de tomates orgânicos (Forty, Andrea e Perinha). Estes foram desidratados e em seguida foram processados na forma de conservas com uso de azeite de oliva como líquido de cobertura e condimentos orgânicos. Os resultados apresentaram produtos com umidade média que variou entre 46 a 63%, pH entre 4,07 e 4,31, cinzas entre 1,04 e 1,87%, proteínas entre 3,01 e 3,94%, lipídeos entre 19,77 e 28,17%, carboidratos entre 12,16 e 23,63% e VCT entre 241 e 348 calorias/100g. A análise sensorial e a intenção de compra demonstraram que as conservas de tomates orgânicos desidratados nos dois equipamentos apresentaram características sensorialmente adequadas para consumo e a comercialização, o que permite afirmar que é uma excelente alternativa para a agricultura familiar.