180 research outputs found
Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity
http://aem.asm.org/Seventy-eight Enterococcus faecium strains from various sources were characterized by random amplified polymorphic DNA (RAPD)-PCR, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis
(PFGE) analysis of SmaI restriction patterns. Two main genomic groups (I and II) were obtained in both RAPD-PCR and AFLP analyses. DNA-DNA hybridization values between representative strains of both groups demonstrated a mean DNA-DNA reassociation level of 71%. PFGE analysis revealed high genetic strain diversity within the two genomic groups. Only group I contained strains originating from human clinical
samples or strains that were vancomycin-resistant or beta-hemolytic. No differentiating phenotypic features
between groups I and II were found using the rapid ID 32 STREP system. The two groups could be further subdivided into, respectively, four and three subclusters in both RAPD-PCR and AFLP analyses, and a high
correlation was seen between the subclusters generated by these two methods. Subclusters of group I were to some extent correlated with origin, pathogenicity, and bacteriocinogeny of the strains. Host specificity of E. faecium strains was not confirmed
Modification of the aggregation behaviour of the environmental Ralstonia eutropha-like strain AE815 is reflected by both surface hydrophobicity and amplified fragment length polymorphism (AFLP) patterns
Screening and characterization of lactic acid bacteria. First Meeting of Contractors BRIDGE T-Project on Lactic Acid Bacteria, Estoril, Portugal, Abstract, 1991.
Polyphasic approaches to the classification of Proteobacteria. ECCO XI Workshop, Göteborg, Sweden, Abstract, 1992.
Classification and identification methods for lactic acid bacteria with emphasis on protein gel electrophoresis
Identification and classification of lactic acid bacteria by sodium dodecyl sulphate polyacrylamide gel-electrophoresis of whole-cell proteins. Associated Lactic Acid Bacteria Industry Platform (LABIP), Book of Abstracts nr P-2, p. 14, Cork, Ireland, 1992.
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