Parechovirus and enteroviruses among young infants with sepsis in Iran

Background and Objectives: Human parechoviruses (HPeV) and Human enteroviruses (EV) frequently cause a sepsis-like illness in young infants (younger than three months). Therefore, this study was conducted to determine the frequency of HPeV and EV among the young infants with clinical signs and symptoms of sepsis in Ahvaz city, Iran. Materials and Methods: The blood specimens were collected from 100 (younger than 90 days hospitalized infants) including 54 (56.25%) males and 46 (43.75%) females with clinical signs and symptoms of sepsis-like disease. The RNA was extracted and tested for detection of VP1 region of HPeV and 5 UTR (Untranslated Region) of EV by RT-PCR. The sequences of positive of HPeV were further analyzed to determine HPeV genotyping. Results: 5/100 (5%) of patients including 2/46 (2%) females and 3/54 (3%) males tested positive for HPeV (P=0.85). The analysis of 5 positive VP1 region of HPeV revealed the genotype 1. The analysis of sequencing and phylogenetic tree revealed that the isolated HPeVs were genotype 1. While 38/100 (38%) specimens including 16 (16%) females and 22 (22%) males were tested positive for EV (P=0.68). Conclusion: The frequency of HPeV genotype 1 was 5% among the young infants with sepsis. While frequency of EV was 38% among the young infants with sepsis. This study showed HPeV genotype 1 and EV are dominant in this region

The healthy behaviours and COVID-19 mortality among Iranian women: A case–control study

Background: Women are among the susceptible groups to Coronavirus disease-19 (COVID-19) in Ardabil, north-west of Iran, despite the current global status. The underlying causes of high incidence and fatality rate of women in Ardabil are not fully understood. Hence, this study aimed to investigate the healthy behaviours in women of Ardabil and its relationship with COVID-19 mortality. Methods: We conducted a case–control study to compare the adherence to health protocols and behaviours with respect to COVID-19 between the infected (261 patients) and healthy (515 persons) women. Health protocols and behaviours such as using mask, gloves, disinfectants, history of travelling and contacting, and attending various gatherings and places during the COVID-19 pandemic along with demographic variables were defined as independent variables, and COVID-19 death rate was defined as the dependent variable. Multivariable logistic regression methods were used to explore the risk factors associated with COVID-19 mortality. Results: Chi-square and Fisher tests showed significant differences between infected and healthy women in terms of history of contact and traveling (p < 0.05), wearing mask (p < 0.001), going to work place (p < 0.001), and attend public gatherings (p = 0.038). Multivariable logistic regression disclosed that the age group over 80 years: 8.97 times (95% CI 2.27–29.85), women with underlying chronic diseases: 4.14 times (95% CI 1.61–10.64), and obese women: 3.01 times (95% CI 1.04–6.03) were more likely to die from COVID-19 than other women. Conclusion: Considering the high incidence and mortality rate in Ardabil women due to COVID-19 and the corresponding health behavioural factors, special emphasis should be given to the increase of women awareness on the importance of healthy behaviours, diet, and life-style

What family doctors know about congenital CMV: a regional survey in Iran

Abstract Background Since there is no effective treatment or vaccine against the congenital cytomegalovirus (cCMV) infection, knowledge and awareness of medical doctor’s (MDs) especially family doctors are essential for preventive strategies and it also seems to be usually ignored by healthcare providers. Aim of this study was to investigate awareness of MDs about cCMV infection in Iran. Methods A single page questionnaire was randomly distributed among 450 MDs including general practitioners, pediatricians, gynecologists, internal and other medical specialists concerning of their knowledge in clinical presentation, diagnosis, prevention, prognosis, epidemiology, transmission, and management of cCMV infection. All statistical analyses were performed using SPSS version 16. Results More than half of questionnaire recipients refused to take part in any of the questionnaire items. The most of the respondents were agreed for newborn CMV screening tests and mandatory CMV test for women trying to get pregnant, which, are not routinely tested. The knowledge of general practitioners about cCMV was less than usual. The field of expertise had a profound effect in this survey, but age and gender did not. Conclusions Our results indicated that the knowledge of cCMV infection, especially among family doctors contains several gaps. Urgent action is required to improve family doctor’s knowledge of CMV infection. Surveys to evaluate CMV awareness among MDs, healthcare professionals and women of childbearing age are proposed

The effect of working-based individual protective behaviors (WIPB) on COVID-19 mortality in north-west of Iran: A case-control study

Background: Given the global emergency for COVID-19 infections, only Workingbased Individual Protective Behaviors (WIPB) such as health behaviors are emphasized as the most important obstacle in spreading the disease. This study aimed to determine the effect of WIPB on COVID-19 mortality.Materials and Methods: This case-control study was conducted on 3088 people (case=1955, control=1133) of the northwest of Iran, Ardabil. Data for infected society people as a case group was collected from medical records retrospectively, and for the control group (noninfected) was obtained from the comprehensive health care system. A designed questionnaire was used to identify WIPB in the Ardabil population during the COVID-19 epidemic. Chi-square test and multivariate logistic regression were used in STATA version 16.Results: The results showed that most patients with COVID-19 in the control group belonged to the Unemployed/Housewives occupational group (27.6%), and the lowest was related to the police officers (2.2%) and transport workers (2.3%). There was a significant difference between groups in terms of sociodemographic factors (p<0.001). Inpatients had used masks, gloves, and disinfectants in the last 14 days by 66.2%, 35.3%, and 44.1%, respectively. There was a significant difference between case and control groups in terms of using a mask (p=0.004), contacting, traveling, and participating in public gatherings (p<0.001).Conclusions: By increasing WIPBs and teaching how to properly observe such behaviors, including the proper use of masks, disinfectants, and gloves in society, as well as intensifying quarantine by preventing gatherings, the incidence and prevalence of COVID-19 can be reduced

Possible Rapid Therapeutics for SARS Coronavirus 2 RNA-dependent RNA polymerase: A Systematic Review: Possible Rapid Therapeutics for SARS Coronavirus 2 …

Research confirms that viral polymerases play a key role in viral genome replication and transcription. Hence, they are essential for the multiplication and survival of viral particles. Our review is limited to original papers in the English language from 2019 to 2020 using various databases, including PubMed, Scopus, Google Scholar, and Science direct. Out of 159 papers, 27 drugs may effectively prevent RNA-dependent RNA polymerase&nbsp;(RdRP), of COVID-19, agreed in order of drug discovery years and paper publication, respectively. In this paper, we realized that the structure comparison and sequence alignment suggest that the mode of substrate RdRP is highly conserved in diverse RNA viruses, providing a foundation for designing broad-spectrum antiviral drugs based on nucleotide analogs

Investigation of SARS-CoV-2 virus on nozzle surfaces of fuel supply stations in North West of Iran

There are several ways for transmitting the SARS-CoV-2 virus to humans, and one of these ways is the contact with infected surfaces. Fuel stations have been a major concern for people and health experts due to the frequent use of common nozzles. This study was performed to identify the COVID-19 virus in the nozzles of fuel supply stations. In the current research, 25 fuel supply stations along the main street ways were investigated to recognize Coronavirus infection on the surfaces of the nozzles. For each fuel supply station, 4 nozzles were chosen (a total of 100 samples). The sampling was carried out at two periods of time, that is, before and after quarantine restrictions. Swapping was used for surface sampling, and Real-time PCR was used to determine the positive and negative results. The results showed that out of nine fuel supply stations (36 samples of nozzle surfaces), five were positive for the presence of SARS-CoV-2 on the nozzle surfaces before the corona restriction. The results showed that in the conditions after corona restriction, all samples were negative in terms of the presence of SARS-CoV-2 on the surfaces of the nozzles. In spite of the fact that gas stations can be one of the foremost inclined places for the transmission and spread of coronavirus due to the nearness and visit of individuals, but through the observance of health behaviors and implementing some procedures, cutting the transmission chain in gas stations can be facilitated

SARS-CoV-2 in municipal wastewater treatment plant, collection network, and hospital wastewater

The current outbreak of coronavirus disease (COVID-19) has led to creating a public health emergency conditions since 2019. COVID-19, which is caused by SARS-CoV-2, is spread via human-to-human transmission by direct contact or droplets. Through conducting this study, we were looking for detecting SARS-CoV-2 in wastewater produced in Iran country (Ardabil, Nir, Khalkhal, and Kowsar) (wastewater collection network, wastewater treatment plant, and hospital wastewater). In this research, samples (n=76) were collected from influent and effluent of municipal and hospital wastewater treatment plants, and some samples were also collected from Ardabil municipal wastewater manholes. The sampling duration included the white (lower risk of COVID-19) and red (high risk of COVID-19) conditions. Samples were stored at −20 °C for further diagnostic tests. The specific primer and probe real-time reverse transcriptase-polymerase chain reaction (real-time PCR) targeting ORF1ab and N genes (nucleoprotein gene) were applied to detect viral genomes of the SARS-CoV-2 virus in the wastewater samples. Out of 76 samples, a total of 15 samples (19.73%) collected from wastewater in Ardabil province (Ardabil, Nir, Khalkhal, and Kowsar), were positive in terms of SARS-CoV-2. Wastewater epidemiology can facilitate detection of the incidence of pathogens through metropolises, measurement of population prevalence without direct testing, and provision of information to the public health system about the efficiency of intervening efforts. [Figure not available: see fulltext.]

Additional file 1: of What family doctors know about congenital CMV: a regional survey in Iran

Questionnaire ‘What family doctors know about congenital CMV: a regional survey in Iran’. (DOC 24 kb