A molecularly imprinted electrochemical biosensor based on hierarchical Ti2Nb10O29 (TNO) for glucose detection

A novel molecularly imprinted electrochemical biosensor for glucose detection is reported based on a hierarchical N-rich carbon conductive-coated TNO structure (TNO@NC ). Firstly, TNO@NC was fabricated by a novel polypyrrole-chemical vapor deposition (PPy-CVD) method with minimal waste generation. Afterward, the electrode modification with TNO@ NC was performed by dropping TNO@NC particles on glassy carbon electrode surfaces by infrared heat lamp. Finally, the glucose-imprinted electrochemical biosensor was developed in presence of 75.0 mM pyrrole and 25.0 mM glucose in a potential range from+ 0.20 to +1.20 V versus Ag/AgCl via cyclic voltammetry (CV). The physicochemical and electrochemical characterizations of the fabricated molecularly imprinted biosensor was conducted by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and CV techniques. The findings demonstrated that selective, sensitive, and stable electrochemical signals were proportional to different glucose concentrations, and the sensitivity of molecularly imprinted electrochemical biosensor for glucose detection was estimated to be 18.93 RA mu M-1 cm(-2) ( R-2 = 0.99) = 0.99) at + 0.30 V with the limit of detection (LOD) of 1.0 x10(-6) M. Hence, it can be speculated that the fabricated glucose-imprinted biosensor may be used in a multitude of areas, including public health and food quality

Electrochemical immunosensor development based on core-shell high-crystalline graphitic carbon nitride@carbon dots and Cd0.5Zn0.5S/d-Ti3C2Tx MXene composite for heart-type fatty acid-binding protein detection

Acute myocardial infarction (AMI) is a significant health problem owing to its high mortality rate. Heart-type fatty acid-binding protein (h-FABP) is an important biomarker in the diagnosis of AMI. In this work, an electrochemical h-FABP immunosensor was developed based on Cd0.5Zn0.5S/d-Ti3C2Tx MXene (MXene: Transition metal carbide or nitride) composite as signal amplificator and core-shell high-crystalline graphitic carbon nitride@carbon dots (hc-g-C3N4@CDs) as electrochemical sensor platform. Firstly, a facile calcination technique was applied to the preparation of hc-g-C3N4@CDs and immobilization of primary antibody was performed on he-g-C3N4@CDs surface. Then, the conjugation of the second antibody to Cd0.5Zn0.5S/d-Ti3C2Tx MXene was carried out by strong pi-pi and electrostatic interactions. The prepared electrochemical h-FABP immunosensor was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) method, Fourier-transform infrared spectroscopy (FTIR), x-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The prepared electrochemical h-FABP immunosensor indicated a good sensitivity with detection limit (LOD) of 3.30 fg mL(-1) in the potential range +0.1 to +0.5 V. Lastly, low-cost, satisfactory stable, and environmentally friendly immunosensor was presented for the diagnosis of acute myocardial infarction

Nanomaterials as the powerful catalysts in electroanalysis

In the rapidly evolving landscape of electroanalysis, the role of nanomaterials has emerged as a transformative force, propelling the field to the upper stages. This special issue delves into the groundbreaking contributions of nanomaterials, exploring their potential as catalysts and their impact on shaping the future of electroanalytical techniques

Sensitive sandwich-type electrochemical SARS-CoV-2 nucleocapsid protein immunosensor

A sensitive and fast sandwich-type electrochemical SARS-CoV-2 (COVID-19) nucleocapsid protein immunosensor was prepared based on bismuth tungstate/bismuth sulfide composite (Bi2WO6/Bi2S3) as electrode platform and graphitic carbon nitride sheet decorated with gold nanoparticles (Au NPs) and tungsten trioxide sphere composite (g-C3N(4)/Au/WO3) as signal amplification. The electrostatic interactions between capture antibody and Bi2WO6/Bi2S3 led to immobilization of the capture nucleocapsid antibody. The detection antibody was then conjugated to g-C3N(4)/Au/WO3 via the affinity of amino-gold. After physicochemically characterization via transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD), and x-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) analysis were implemented to evaluate the electrochemical performance of the prepared immunosensor. The detection of SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 NP) in a small saliva sample (100.0 mu L) took just 30 min and yielded a detection limit (LOD) of 3.00 fg m(-1), making it an effective tool for point-of-care COVID-19 testing

A Comparative 3D Finite Element Computational Study of Stress Distribution and Stress Transfer in Small-Diameter Conical Dental Implants

The implant design is one of the main factors in implant stability because it affects the contact area between the bone and the implant surface and the stress-strain distribution at the bone-implant interface. In this study, the effect of different groove geometries on stress-strain distributions in small-diameter conical implants is investigated using the finite element method (FEM). Four different thread models (rectangular, buttressed, reverse buttressed, and symmetrical profile) are created by changing the groove geometry on the one-piece implants, and the obtained results are compared. The stress shielding effect is investigated through the dimensionless numbers that characterize the load-sharing between the bone-implant. It is determined that the lowest stress distribution is observed with rectangular profiled groove geometry. Besides, it is obtained that the buttressed groove geometry minimizes the stress effects transmitted to the periphery of the implant. The symmetrical profiles had better performance than rectangular profiles in stress transfer

CD11b Expression in Acute Myeloid Leukemia is Associated With Hemostatic Complications and Response to Treatment

Aim:In our study, we aimed to investigate the effects of CD11b expression on myeloblasts on clinical course and prognosis in patients with AML.Materials and Methods:Data of 123 patients diagnosed with AML between 2014-2017 in Trakya University Faculty of Medicine, Department of Hematology, a tertiary referral hospital in the Trakya Region, were evaluated in a retrospective manner. The diagnosis of AML was based on WHO 2016 criteria of Myeloid Neoplasms.Results:Of the 123 patients in our study, 60 were female, and 63 were male. The mean age was 57.93 years. CD11b positivity was observed in 40 patients. Platelet counts were significantly lower in patients with CD11b positivity (p = 0.004). Likewise, D-dimer levels at presentation were higher in the CD11b positive patient group (p = 0.000). Regarding outcomes, patients with CD11b positivity were found to have lower rates of remission with first-line remission induction therapy (p = 0.003). There was no significant relationship between CD11b positivity and overall survival with Kaplan Meier survival analysis (8.5 months in CD 11b positive group, 12.1 months in negative group, p: 0.436).Conclusion:Our study demonstrated that patients with CD11b expression had lower remission rates with remission induction chemotherapy

Could ratio of hemoglobin to red cell distribution width and ratio of absolute lymphocyte count to absolute monocyte count be a prognostic tool in newly diagnosed multiple myeloma patients?

IntroductionHemoglobin/red cell distribution width (RDW) ratio (HRR) and lymphocyte-to-monocyte ratio (LMR) are two novel bio-markers associated with overall survival (OS) and prognosis in several types of cancers. The aim of this study is to investigate the value of HRR and LMR in newly diagnosed multiple myeloma (MM) patients. MethodsA total of 180 patients were included in this study. Patients diagnosed with MM between May 2013 and May 2019 at a single center were evaluated. HRR was calculated by dividing hemoglobin to RDW, both measured from the same sample. LMR was calculated by dividing absolute lymphocyte count (ALC) to absolute monocyte count (AMC). ResultsThe cutoff value for HRR was taken as 0.61, and the cutoff value for LMR was taken as 3.28. Patients were divided into low HRR, high HRR, low LMR, and high LMR groups. OS of the patients with low HRR was found lower compared with high HRR (36.7 months for low HRR and 53.2 months for high HRR, < 0.001). Also, OS was found lower in the low LMR group (39.4 months for low LMR and 51.7 months for high LMR, = 0.016). On multivariate analysis, low HRR and low LMR were predictive factors of OS (hazard ratio (HR) 2.08, 95% confidence intervals (CI) 1.31–3.03, and = 0.002 for low HRR; HR 1.47, 95% CI 0.92–2.29, and = 0.010 for low LMR). ConclusionCombining both HRR and LMR could be a prognostic biomarker and it reflects the status of the immune system in newly diagnosed MM patients

Sex difference and intra-operative tidal volume: Insights from the LAS VEGAS study

BACKGROUND: One key element of lung-protective ventilation is the use of a low tidal volume (VT). A sex difference in use of low tidal volume ventilation (LTVV) has been described in critically ill ICU patients.OBJECTIVES: The aim of this study was to determine whether a sex difference in use of LTVV also exists in operating room patients, and if present what factors drive this difference.DESIGN, PATIENTS AND SETTING: This is a posthoc analysis of LAS VEGAS, a 1-week worldwide observational study in adults requiring intra-operative ventilation during general anaesthesia for surgery in 146 hospitals in 29 countries.MAIN OUTCOME MEASURES: Women and men were compared with respect to use of LTVV, defined as VT of 8 ml kg-1 or less predicted bodyweight (PBW). A VT was deemed 'default' if the set VT was a round number. A mediation analysis assessed which factors may explain the sex difference in use of LTVV during intra-operative ventilation.RESULTS: This analysis includes 9864 patients, of whom 5425 (55%) were women. A default VT was often set, both in women and men; mode VT was 500 ml. Median [IQR] VT was higher in women than in men (8.6 [7.7 to 9.6] vs. 7.6 [6.8 to 8.4] ml kg-1 PBW, P &lt; 0.001). Compared with men, women were twice as likely not to receive LTVV [68.8 vs. 36.0%; relative risk ratio 2.1 (95% CI 1.9 to 2.1), P &lt; 0.001]. In the mediation analysis, patients' height and actual body weight (ABW) explained 81 and 18% of the sex difference in use of LTVV, respectively; it was not explained by the use of a default VT.CONCLUSION: In this worldwide cohort of patients receiving intra-operative ventilation during general anaesthesia for surgery, women received a higher VT than men during intra-operative ventilation. The risk for a female not to receive LTVV during surgery was double that of males. Height and ABW were the two mediators of the sex difference in use of LTVV.TRIAL REGISTRATION: The study was registered at Clinicaltrials.gov, NCT01601223

Dershane öğretmenlerinin iş tatmin düzeylerinin belirlenmesi

Bu araştırmada amacı, dershanelerde çalışan öğretmenlerin çalıştıkları dershanelerdeki iş doyumu düzeylerini saptamak amaçlanmıştır. Bunun yanında dershanelerde çalışan öğretmenlerin iş doyumu düzeylerinin hangi boyutlarda düşük olduğunu, hangi değişkenlere göre farklılaşma gösterdiğini belirlemek ve önerilerde bulunmak da hedeflenmiştir. Araştırmada tarama modeli kullanılmıştır. Örneklemde “küme örnekleme” yöntemi kullanılmıştır. Araştırma evrenini, 2018-2019 öğretim yılında Denizli İli Pamukkale ve Merkezefendi İlçelerinde’nde bulunan dershanelerde görev yapan öğretmenler oluşturmaktadır. Dershane ortamındaki eğitim öğretimde lokomotif rol oynayan öğretmenlerin memnuniyetini saptayıp, morallerinin yüksek tutulması konusunda gerekli önlemleri alarak, bu çalışanların eğitim ortamına katkılarını artırmak hedeflenmektedir. Araştırmada Minne Sota İş Doyum Ölçeği, dershane öğretmenlerine uyarlanarak kullanılmıştır. Denizli ili Pamukkale ve Merkezefendi ilçeleri bir küme olarak ele alınmıştır. Dershane öğretmenleri ve yöneticileriyle iletişim sağlanarak, gönüllülük esasına göre anket çalışması yapılmıştır. Çalışmada eğitim alanında iş doyumu kavramlarının önemi kuramsal ve kavramsal analizlerle vurgulanmaya çalışılmıştır. Ayrıca, iş doyumu düzeyleri alt boyutları ile tespit edilmiştir

Dosimetric effects of x-rays for patients with plaster that take radiotheraphy

Bu çalışmada, vücudun alçıya alınmış çeşitli bölgelerine verilmesi muhtemel radyasyon dozunun iyi planlanabilmesi amacıyla, Siemens marka, Oncor Impression model lineer hızlandırıcı tedavi cihazında, 6 MV ve 18 MV enerjili x-ışınları kullanılarak yüzde derin doz ölçümleri yapılmıştır. Doku eşdeğeri olan katı fantom ve alçılı katı fantomun kalınlıkları (5x5) cm2, (10x10) cm2 ve (15x15) cm2 alan açıklıkları için, değiştirilerek alçının kütle azalma katsayısı deneysel olarak elde edilmiş ve alçının doza etkisi araştırılmıştır. Dozun derinliğe karşılık çizilen grafiklerden lineer azalma katsayısının (&#956;) enerjinin artması ile azaldığı görülmüştür. NIST x-ray programı kullanılarak alçının azalma katsayısı teorik olarak hesaplanmış ve deneysel bulgular ile karşılaştırılmıştır. (5x5) cm2'lik alan açıklığında sonuçların birbiri ile uyumlu ve saçılmaların da bu alan açıklığı için en az olduğu görülmüştür.In this work, the percent depth dose measurements were done by using x-rays with 6 MV and 18 MV from linear accelerator therapy device (Siemens, Oncor Impression model) to plan the probable radiation dose to be given to patient with plaster. By changing the thickness of tissue equivalent solid phantom with and without plaster the linear attenuation coefficients were experimentally determined for (5x5) cm2, (10x10) cm2 and (15x15) cm2 areas and investigated the effect of plaster to dose. It was shown that the linear attenuation coefficients are decreasing while energy is increasing. The linear attenuation coefficient of plaster was also calculated by using the NIST x-ray program and compared with the measured values. The results were found that they are good agreement with each other's and the back scattering are the less for (5x5) cm2 than for that of other areas