Experimental studies of proton-neutron mixed symmetry states in the mass A ∼ 130 region

Considerable progress has been achieved recently in the experimental investigation of quadrupole-collective isovector excitations in the valence shell, the so called mixed-symmetry states (MSSs), in the mass A ≈ 130 region. This is due to a new experimental technique for study MSSs which is based on the observation of low-multiplicity γ-ray events from inverse kinematics Coulomb excitation with the large 4π spectrometer, such as Gammasphere. The obtained experimental information for the MSSs of stable N 80 isotones indicates that for low-collective vibrational nuclei the underlying single-particle structure can be the most important factor for preserving or fragmenting the MSSs through the mechanism of shell stabilization. The evolution of the MSSs from 134Xe to 138Ce is also used to determine the local strength of the proton-neutron interaction derived for first time from states with symmetric and antisymmetric nature

Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta)

Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection

Transcription of DNA-histone complexes by yeast RNA polymerase B.

Transcription of denatured DNA complexed with histones (total, H1 or H2A/H2B/H3/H4) by yeast RNA polymerase B is investigated. Binding of histones to DNA restricts its template activity by decreasing the formation of active, heparin-resistant, RNA polymerase initiation complexes. The elongation of pre-initiated RNA on denatured DNA, complexed with histones, is possible, although resulting in somewhat shorter RNA chains. It is suggested that RNA polymerase B can elongate on a DNA strand covered with histones

Expression and localization of PCSK9 in rat hepatic cells.

Proprotein convertase subtilisin/kexin type 9 (PCSK9), recently cloned in several laboratories, including ours, causes a third form of autosomal dominant hypercholesterolemia. Its mechanism of action remains unclear. We studied the expression and subcellular localization of PCSK9 in fetal and adult rat tissues associated with cholesterol homeostasis using quantitative reverse transcriptase--PCR, Western blot analysis, subcellular fractionation, and confocal immunofluorescent microscopy. PCSK9 mRNA is most abundant in yolk sac and fetal liver, but the highest expression of the protein was found in differentiated hepatoma FAO-1 cell line, which also shows the highest expression of LDLR. In FAO-1 cells PCSK9 expression is downregulated by cholesterol and 25-hydroxycholesterol and upregulated in the absence of sterols following the same pattern of expression as HMG-CoA reductase, synthase, and LDLR. Subcellular fractionation, combined with Western blotting, showed that PCSK9 is localized in the ER and intermediate vesicular compartment of the cell but not in Golgi cisternae. The mature enzyme is secreted from the liver and hepatoma cells. Double labeling with antibodies to PCSK9 and LDLR or clathrin revealed some colocalization of PCSK9 with clathrin-coated vesicles and LDLR. In conclusion, our results show that PCSK9 is processed in the ER, and the mature convertase is secreted in the plasma

Experimental studies of proton-neutron mixed symmetry states in the mass A ≈ 130 region

Considerable progress has been achieved recently in the experimental investigation of quadrupole-collective isovector excitations in the valence shell, the so called mixed-symmetry states (MSSs), in the mass A ≈ 130 region. This is due to a new experimental technique for study MSSs which is based on the observation of low-multiplicity γ-ray events from inverse kinematics Coulomb excitation with the large 4π spectrometer, such as Gammasphere. The obtained experimental information for the MSSs of stable N = 80 isotones indicates that for low-collective vibrational nuclei the underlying single-particle structure can be the most important factor for preserving or fragmenting the MSSs through the mechanism of shell stabilization. The evolution of the MSSs from ¹³⁴Xe to ¹³⁸Ce is also used to determine the local strength of the proton-neutron interaction derived for first time from states with symmetric and antisymmetric nature

Experimental studies of proton-neutron mixed symmetry states in the mass A ≈ 130 region

Considerable progress has been achieved recently in the experimental investigation of quadrupole-collective isovector excitations in the valence shell, the so called mixed-symmetry states (MSSs), in the mass A ≈ 130 region. This is due to a new experimental technique for study MSSs which is based on the observation of low-multiplicity γ-ray events from inverse kinematics Coulomb excitation with the large 4π spectrometer, such as Gammasphere. The obtained experimental information for the MSSs of stable N = 80 isotones indicates that for low-collective vibrational nuclei the underlying single-particle structure can be the most important factor for preserving or fragmenting the MSSs through the mechanism of shell stabilization. The evolution of the MSSs from ¹³⁴Xe to ¹³⁸Ce is also used to determine the local strength of the proton-neutron interaction derived for first time from states with symmetric and antisymmetric nature