Investigation of the fatty acid amounts in the bee pollens of collected from Bingol’s flora

Bu çalışmada, Bingöl yöresinin beş farklı yerinden toplanan arı poleni örneklerindeki yağ asidi bileşenleri araştırıldı. Çalışmada beş farklı yerin her birinden 4 örnek olmak üzere toplam 20 örnek kullanıldı. Polen örnekleri estraksiyon işlemi yapıldı ve ekstraksiyonlar gaz kromatografisiyle analiz edildi. Analiz sonuçlarına göre, palmitik asit (C16:0), stearik asit (C18:0), oleik asit (C18:1n-9) ve γ – linolenik asit (C18:3n-6) miktarlarında istatistiksel olarak anlamlı bir fark (p<0.05) saptanırken, palmitoleik asit (C16:1n-7), linoleik asit (C18:2n-6), α – linolenik asit (C18:3n-3), toplam doymuş yağ asidi (SFA), toplam tekli doymamış yağ asidi (MUFA) ve toplam çoklu doymamış ( PUFA) yağ asidi miktarlarındaki farklılıklar istatistiksel olarak önemsiz bulundu (p>0.05). Sonuç olarak, polen örneklerindeki yağ asitlerinin doymamışlık oranının doymuşluğa oranına olan değerinin (TUFA/SFA) 1.57 - 1.92 arasında olduğu ve esansiyel yağ asitlerinden α – linolenik asit ve γ – linolenik asit karışımının toplam yağ asitlerinin yaklaşık % 33’ü ve linoleik asitin toplam yağ asitlerinin yaklaşık % 10’u olduğu saptandı. Bu araştırmayla bölgedeki polenlerin biyolojik değeri ve besin kalitesi hakkında bilgi edinilebileceği ve bu araştırma sonuçlarının literatür bilgisine katkıda bulunabileceği düşünülmektedirIn this study, fatty acid compounds in the bee pollens collected from five different locations of Bingöl were investigated. A total of 27 samples of polen, each 4 of which taken from 5 different places, were examined. Pollen samples were extracted and extractions were analyzed by gas choromatography (GC). According to results, while significant difference in the amount of palmitic acid (C16:0) and stearic acid (C18:0) oleic acid (C18:1n-9) and γ – linolenic acid (C18:3n-6) were found, the amounts of palmitoleic acid ( C16:1n-7), linoleic acid (C18:2n-6), α – linolenic acid (C18:3n-3), saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were not found significant (p>0.05). As a result, it was found that the unsaturated-to-saturated fatty acids ratio (TUFA/SFA ratio) in the pollen samples examined ranged from 1.57 to 1.92 and essential fatty acids appeared to be mixture of α-linolenic acid and γ- linolenic acid, 33% and linoleic acid 10 %, respectively of the total fatty acids. It can be thought that with this study one can get information about biological value and nutritional quality of the pollen grains in the this region and this investigation will have an important effect on improvement of the knowledge of that area

Effects of The Hydroxyurea Derivative 1, 3, 4- Thiadiazoles On Antioxidant Vitamins, MDA in Serums of Rats and Cell Viability of MCF-7 Breast Cancer Cells

In this study, the effects of as used ligand [1-hydroxy - 2 - (5 - (trifluoromethyl) - 1, 3, 4- thiadiazole- 2 - yl)guanidine] and its Mn, Cd, Cr complexes on the antioxidant vitamins and MDA levels in the serums of rats and antitumor activity of these chemical compounds on the MCF-7 breast cancer cells at different concentrations were investigated. When the results were compared between the levels of control and experimental groups of antioxidant vitamins ve MDA statistically, it was observed that there were decreased levels of A, E, and C vitamins in groups of applied Cd (II) and Cr (II) complexes compared with the control group, whereas MDA levels were increased. The antioxidant vitamins and MDA levels in serum were determined by HPLC.It was clearly observed that the subcutaneously MCF-7 breast cancer cells treated Ligand (L), Mn(L)2, Cd(L)2 and Cr(L)2 complexes had the low levels of cell viability activity when compared to the untreated control cells clearly increased according to the control group for after incubation 24 h onwards to 48 h. Cell vialities measured by electronic microscope. As a result, It suggested that thiadiazole compounds exhibit antitumor activity with reduction in serum antioxidant vitamins of rats can cause cytotoxic effect depending on the mechanism of oxidative damage

Türkiye’de tabiat tarihi müzeleri

Ankara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2014.This work is a student project of the The Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.by Yiğit, Aslı

The Effects of Sodium Tetraborate against Lead Toxicity in Rats: The Behavior of Some Metabolic Enzymes

This study was planned to research the in vivo effects of lead (Pb) ions and sodium tetraborate (Na2B4O7) on G6PD and 6PGD, which are some of the enzymes of the pentose phosphate pathway, which carries vital importance for metabolism, and GR and GST, which are glutathione metabolism enzymes, and the in vitro effects of the same agents on the 6PGD enzyme. According to the in vivo analysis results, in comparison to the control group, the rat liver G6PD (p 0.05). The Pb group had lower G6PD and 6PGD enzyme activity levels and higher GR and GST enzyme activity levels compared to the control group, while these changes did not reach statistical significance (p > 0.05). In the in vitro analyses of the effects of Pb ions on the 6PGD enzyme that was purified out of rat liver with the 2′,5′-ADP-Sepharose 4B affinity chromatography method, it was determined that Pb ions (200-1200 μM) increased the rat liver 6PGD enzyme activity levels by 33%. On the other hand Na2B4O7 was not significantly effective on 6PGD activity. These results will also contribute to future studies in understanding the physiopathology of the states triggered by Pb ions and sodium tetraborate (Na2B4O7)