A Comparative Analysis on the Innate Immune Responses of <i>Cirrhinus mrigala</i> Challenged with <i>Pseudomonas aeruginosa</i> and <i>Fusarium oxysporum</i>

Microbes are the most significant ubiquitous pathogens that cause serious infections in freshwater fish, leading to tremendous economic losses. The present study was designed to investigate the extent of changes in cytokine expression, hemato-biochemical parameters, and tissue histology of Cirrhinus mrigala (C. mrigala) challenged with Pseudomonas aeruginosa (P. aeruginosa) and Fusarium oxysporum (F. oxysporum). Fish were divided into three major groups: control, P. aeruginosa-challenged, and F. oxysporum-challenged. The infection in both challenge assays was allowed to progress until 7 days post infection. Upregulated expression of TNF-α and IL-1β was found in blood, gills, livers, and kidneys of the challenged fish. Significant differences were noted in hematological parameters of challenged fish. Alanine aminotransferase, aspartate aminotransferase, and alkaline aminotransferase levels also showed significant differences in infected and control groups. An increase in serum albumin and globulin and a decrease in total protein were noted in infected groups as compared to the control group. Severe histological alterations were noted in gill, liver, and kidney tissues of the infected groups as compared to control. The order of histological alteration index for P. aeruginosa challenge was liver &gt; kidney &gt; gills, and for F. oxysporum challenge it was kidney &gt; liver &gt; gills. These changes in fish infected by P. aeruginosa and F. oxysporum can be used as an effective and subtle index to monitor the physiological and pathological conditions of fish

Immunomodulatory Effects of Psyllium Extract on Helicobacter pylori Interaction With Gastric Epithelial Cells.

Natural plant product Psyllium has anti-inflammatory activity that can modulate the function of cytokines. We determined the effect of Psylliumhusk extract on interleukin (IL)-8 and NF-κB secretion by gastric epithelial cells in response to Helicobacter pylori Human gastricadenocarcinoma cell line (AGS) cells were pretreated with Psyllium extract in different concentrations before H pylori infection. Cell culture supernatant was analyzed for IL-8 and NF-κB by ELISA. RNA from cells was used for real-time polymerase chain reaction for messenger RNA expression of IL-8. Psyllium extract 5 and 10 μg/mL markedly (P \u3c .001) lowered basal IL-8 by 64.71% and 74.51%, respectively, and H pylori-stimulated IL-8 was also (P \u3c .001) lowered by 41.67% and 66.67%, respectively. Psyllium 5 and 10 μg/mL also reduced (P \u3c .0001) cagA-positive H pylori-induced IL-8 mRNA expression by 42.3% and 67.6%, respectively. Psyllium also reduced (P = .0001) NF-κB in response to H pylori strains confirming its role as an anti-inflammatory agent

Differences in angiographic profile and immediate outcome of primary percutaneous coronary intervention in otherwise risk-free young male smokers

Introduction: Cigarette smoking is a well-established risk factor for the development and progression of coronary artery disease (CAD) and it is strongly related to cardiac morbidity and mortality. Therefore, this study aimed to compare the angiographic profile and immediate clinical outcomes in young male smokers and non-smokers without any other cardiac risk factors presented with ST-elevation myocardial infarction (STEMI).Methods: This study includes young (≤40 years) male patients presented without cardiac risk factors other than smoking. Angiographic profile and immediate outcome of primary percutaneous coronary intervention (PCI) were collected from the hospital database.Results: A total of 580 young male patients were included in this study, 51.2% (297) were smokers. Baseline characteristics and presentation were similar for smoker and non-smoker groups. Angiographic profile was not significantly different for smokers in terms of pre-procedure thrombolysis in myocardial infarction (TIMI) flow (p = 0.373), the number of vessels involved (p = 0.813), infarct-related artery (p = 0.834), and left ventricular dysfunction (p = 0.311). Similarly, in-hospital outcomes of primary PCI were not significantly different in smokers. Post-procedure no-reflow was in 3.4% vs. 2.8%; p = 0.708, acute stent thrombosis in 1.7% vs. 0.4%; p = 0.114 and in-hospital mortality in 1.0% vs. 1.4%; p = 0.657 of the smoker and non-smoker group, respectively.Conclusion: Our study concludes smoking has no significant impact on the angiographic profile and immediate clinical outcomes of primary PCI after STEMI in young males, without any other conventional cardiac risk factors. With these findings, further multicenter prospective studies are needed to identify other potential causes in such patients

RNA sequencing of FACS-sorted immune cell populations from zebrafish infection models to identify cell specific responses to intracellular pathogens

Animal science

Colorectal Cancer-Associated Genes Are Associated with Tooth Agenesis and May Have a Role in Tooth Development

Previously reported co-occurrence of colorectal cancer (CRC) and tooth agenesis (TA) and the overlap in disease-associated gene variants suggest involvement of similar molecular pathways. Here, we took an unbiased approach and tested genome-wide significant CRC-associated variants for association with isolated TA. Thirty single nucleotide variants (SNVs) in CRC-predisposing genes/loci were genotyped in a discovery dataset composed of 440 individuals with and without isolated TA. Genome-wide significant associations were found between TA and ATF1 rs11169552 (P = 4.36 × 10−10) and DUSP10 rs6687758 (P = 1.25 × 10−9), and positive association found with CASC8 rs10505477 (P = 8.2 × 10−5). Additional CRC marker haplotypes were also significantly associated with TA. Genotyping an independent dataset consisting of 52 cases with TA and 427 controls confirmed the association with CASC8. Atf1 and Dusp10 expression was detected in the mouse developing teeth from early bud stages to the formation of the complete tooth, suggesting a potential role for these genes and their encoded proteins in tooth development. While their individual contributions in tooth development remain to be elucidated, these genes may be considered candidates to be tested in additional populations

Maritime cybersecurity: are onboard systems ready?

Recent maritime cybersecurity accidents reveal that shipping is facing increased exposure to cyber threats, especially due to the fast-growing digitalisation of the sector, leaving vessels and their onboard systems vulnerable to cyberattacks. This research aims at evaluating the relationship among the critical dimensions influencing cybersecurity performance in the maritime industry. To achieve this, six critical dimensions related to cybersecurity preparedness are first identified through literature review, namely ‘regulations’, ‘company procedures’ from a managerial perspective and ‘shipboard systems readiness’, ‘training and awareness’, ‘human factor’ and ‘compliance monitoring’ at an operation level. A Likert-scale questionnaire is designed and used to collect empirical data from 133 seafarers and shore-based staff. Structural Equation Modelling (SEM) is applied to examine the causal relationships between the six dimensions and shipboard cybersecurity performance. The results show that ‘regulations’ positively influence shipping companies’ cybersecurity-related ‘procedures’, which in turn positively affects ‘shipboard systems readiness’, ‘training and awareness’, and ‘monitoring’. Further, ‘training and awareness’ positively influences the cybersecurity performance of ships. The results have profound implications for the shipping industry on how to strengthen their cyber practices in order to improve their cybersecurity performance. Recommendations for future academic research related to maritime cybersecurity are also provided

MicroRNA-146 function in the innate immune transcriptome response of zebrafish embryos to Salmonella typhimurium infection

Animal science

Assessment of essential minerals and physico-chemical analysis of floral origins fresh honey produced by Apis mellifera

Abstract Honey is one of the best nutritious substances in the world, having different services in the body functions regulation. Ten elements (K, Na, Ca, Co, Cr, Mn, Mo, Ni, Pb, Se) from honey samples were analyzed from 80 different locations of Punjab and ten floras. The aim of the present study was to determine the quality and quantity of minerals and Physico-chemical analysis in honey. A flame photometer was used to measure the concentration of major minerals (K, Ca and Na). The concentration of micro minerals (Co, Cr, Mn, Mo, Ni, Pb and Se) was analyzed using Atomic Absorption Spectrometer. The concentration of macro-elements obtained was as follow (in ppm): K (166-1732), Na (107-418) and Ca (07-99), while the concentration of microelements (in ppm) Co (1-2), Cr (>1), Mn ( Co > Ni > Pb > Cr > Mo > Mn. The findings showed that all the heavy metals like Co, Cr, Ni and Pb were present in trace amounts and close to International Honey Quality Standard. The result of given honey samples represented highest value of moisture (31.23%), color (80 mm pfund), pH (8.23), acidity (72.02 meq/kg), electrical conductivity (0.85 ms/cm) and ash contents (0.83%)

Modified physical properties of Ni doped ZnO NPs as potential photocatalyst and antibacterial agents

Hazardous organic dyes, present in the effluents of industries, are continuously polluting the environment. Photodegradation of these dyes on catalyst surface under sunlight irradiation is economic, safe and suitable strategy to protect environment. Hence, the synthesis and applications of Zn1-xNixO (x = 0.00, 0.02, 0.04, 0.06) NPs are reported here. Effect of pH and dopant concentration was studied to modify the electrical, magnetic, antibacterial and photocatalytic properties of Ni doped ZnO NPs. The samples were characterized by Scanning electron microscopy (SEM), Powder X-ray diffraction (XRD), UV–Visible spectroscopy (UV–Vis.), Energy-dispersive X-ray spectroscopy (EDS) and Fourier-transform infrared spectroscopy (FTIR) to determine the morphology, crystallite structure, optical properties, elemental composition and functional group detection, respectively. LCR meter and VSM were used to evaluate the dielectric properties and magnetic properties of Ni doped ZnO NPs, respectively. XRD pattern confirmed the presence of hexagonal wurtzite geometry of ZnO NPs. The structural and morphological analysis showed the increase in crystallinity with little effect on shape of doped NPs by increasing the dopant concentration and slight increase of pH. It was observed that the Ni doped ZnO NPs possess good photocatalytic potential by 94% degradation of 20 ppm solution of methyl orange dye (MO) in just 80 min under sunlight. Moreover, the enhancement in antibacterial potential was also observed with increase in dopant concentration and decrease in crystallite size of doped ZnO NPs. Smaller size NPs were found more effective against gram negative bacterial strains. © 2023 The Author(s)King Saud University, KSUAuthors would like to extend their sincere appreciation to the Researchers Supporting Project number (RSP2023R123), King Saud University, Riyadh, Saudi Arabia