Cytokinin transporters: GO and STOP in signaling

Cytokinins are phytohormones essential for cytokinesis and many other physiological and developmental processes in planta. Long-distance transport and intercellular transport have been postulated. For these processes, the existence of cytokinin transporters has been suggested. Recently, a transporter loading the xylem (AtABCG14) and another for cellular import (AtPUP14) have been discovered. AtABCG14 participates in the xylem loading process of cytokinins and contributes to the positive regulation of shoot growth. The cellular importer AtPUP14 is required to suppress cytokinin signaling. A role of a transporter as stop signal is a new paradigm for a hormone transporter

CloudIoT-based Jukebox Platform: a music player for mobile users in Café

Contents services have been provided to people in a variety of ways. Jukebox service is one of the contents streaming which provides an automated music-playing service. User inserts coin and presses a play button, the jukebox automatically selects and plays the record. The Disk Jockey (DJ) in Korean cafeteria (café) received contents desired of customer and played them through the speakers in the store. In this paper, we propose a service platform that reinvented the Korean café DJ in an integrated environment of IoT and cloud computing. The user in a store can request contents (music, video, and message) through the service platform. The contents are provided through the public screen and speaker in the store where the user is located. This allows people in the same location store to enjoy the contents together. The user information and the usage history are collected and managed in the cloud. Therefore, users can receive customized services regardless of stores. We compare our platform to exist services. As a result of the performance evaluation, the proposed platform shows that contents can be efficiently provided to users and adapts IoT-Cloud integrated environments.N/

Production of active Exendin-4 in Nicotiana benthamiana and its application in treatment of type-2 diabetics

GLP-1 (Glucagon-like peptide-1) is a peptide that stimulates insulin secretion from the β-cell for glycemic control of the plasma blood glucose level. Its mimetic exenatide (synthetic Exendin-4) with a longer half-life of approximately 3.3–4 h is widely used in clinical application to treat diabetes. Currently, exenatide is chemically synthesized. In this study, we report that the GLP-1 analogue recombinant Exendin-4 (Exdn-4) can be produced at a high level in Nicotiana benthamiana, with an estimated yield of 50.0 µg/g fresh biomass. For high-level expression, we generated a recombinant gene, B:GB1:ddCBD1m:8xHis : Exendin-4 (BGC : Exdn-4), for the production of Exendin-4 using various domains such as the BiP signal peptide, the GB1 domain (B1 domain of streptococcal G protein), a double cellulose binding domain 1 (CBD1), and 8 His residues (8xHis) to the N-terminus of Exendin-4. GB1 was used to increase the expression, whereas double CBD1 and 8xHis were included as affinity tags for easy purification using MCC beads and Ni(2+)-NTA resin, respectively. BGC : Exdn-4 was purified by single-step purification to near homogeneity using both Ni(2+)-NTA resin and microcrystalline cellulose (MCC) beads. Moreover, Exdn-4 without any extra residues was produced from BGC : Exdn-4 bound onto MCC beads by treating with enterokinase. Plant-produced Exdn-4 (Exendin-4) was as effective as chemically synthesized Exendin-4 in glucose-induced insulin secretion (GIIS) from mouse MIN6m9 cells a pancreatic beta cell line

Proteomic analysis of pregnancy-related proteins from pig uterus endometrium during pregnancy

Many important molecular events associated with implantation and development occur within the female reproductive tract, especially within the uterus endometrium, during pregnancy periods. The endometrium includes the mucosal lining of the uterus, which provides a suitable site for implantation and development of a fertilized egg and fetus. To date, the molecular cascades in the uterus endometrium during pregnancy periods in pigs have not been elucidated fully. In this study, we compared the functional regulated proteins in the endometrium during pregnancy periods with those in non-pregnant conditions and investigated changes in expression patterns during pregnancy (days 40, 70, and 93) using two-dimensional gel electrophoresis (2-DE) and western blotting. The functional regulated proteins were identified and discovered from differentially expressed proteins in the uterus endometrium during pregnancy. We discovered 820 protein spots in a proteomic analysis of uterus endometrium tissues with 2-DE gels. We identified 63 of the 98 proteins regulated differentially among non-pregnant and pregnant tissues (matched and unmatched spots). Interestingly, 10 of these 63 proteins are development-, cytoskeleton- and chaperon-related proteins such as transferrin, protein DJ-1, transgelin, galectin-1, septin 2, stathmin 1, cofilin 1, fascin 1, heat shock protein (HSP) 90β and HSP 27. The specific expression patterns of these proteins in the endometrium during pregnancy were confirmed by western blotting. Our results suggest that the expressions of these genes involved in endometrium function and endometrium development from early to late gestation are associated with the regulation of endometrium development for maintaining pregnancy

Abscisic acid is a substrate of the ABC transporter encoded by the durable wheat disease resistance gene Lr34

The wheat Lr34res allele, coding for an ATP-binding cassette transporter, confers durable resistance against multiple fungal pathogens. The Lr34sus allele, differing from Lr34res by two critical nucleotide polymorphisms, is found in susceptible wheat cultivars. Lr34res is functionally transferrable as a transgene into all major cereals, including rice, barley, maize, and sorghum. Here, we used transcriptomics, physiology, genetics, and in vitro and in vivo transport assays to study the molecular function of Lr34. We report that Lr34res results in a constitutive induction of transcripts reminiscent of an abscisic acid (ABA)-regulated response in transgenic rice. Lr34-expressing rice was altered in biological processes that are controlled by this phytohormone, including dehydration tolerance, transpiration and seedling growth. In planta seedling and in vitro yeast accumulation assays revealed that both LR34res and LR34sus act as ABA transporters. However, whereas the LR34res protein was detected in planta the LR34sus version was not, suggesting a post-transcriptional regulatory mechanism. Our results identify ABA as a substrate of the LR34 ABC transporter. We conclude that LR34res-mediated ABA redistribution has a major effect on the transcriptional response and physiology of Lr34res-expressing plants and that ABA is a candidate molecule that contributes to Lr34res-mediated disease resistance

Pulmonary Artery Embolotherapy in a Patient with Type I Hepatopulmonary Syndrome after Liver Transplantation

Although liver transplantation (LT) is the only effective treatment option for hepatopulmonary syndrome (HPS), the post-LT morbidity and mortality have been high for patients with severe HPS. We performed post-LT embolotherapy in a 10-year-old boy who had severe type I HPS preoperatively, but he failed to recover early from his hypoxemic symptoms after an LT. Multiple embolizations were then successfully performed on the major branches that formed the abnormal vascular structures. After the embolotherapy, the patient had symptomatic improvement and he was discharged without complications

The March Family

Report prepared as part of the coursework required for REL 167, Speaking Stones, Fall 2017.Description and history of the March family. Details about the lives of Dr. John J. March the father, Thelma L. March the mother, and John J. Jr. March the son

Pricing for past channel state information in multi-channel cognitive radio networks

Cognitive Radio (CR) networks have received much attention as a solution to the spectrum inefficiency problem of current license-based regulatory management. In CR networks, Secondary User (SU) can use a spectrum vacancy that can be detected by either sensing-before-transmission or database access. However, sensing inaccuracy or long access time to database often becomes a major obstacle to timely detect the spectrum vacancy. In this paper, we develop a hybrid detection framework in multi-channel CR networks, where an SU can selectively sense a channel for spectrum vacancy by accessing the spectrum history of Markovian channels. We focus on the value of the channel history information offered by the Primary Provider (PP) of each channel, and consider a market for the information between multiple PPs and SU. We investigate the interplay between of the PPs and the SU through their pricing and buying decisions for the information, in the presence of sensing inaccuracy, i.e., false alarm and miss detection

Cytokinin Transporters: GO and STOP in Signaling

Cytokinins are phytohormones essential for cytokinesis and many other physiological and developmental processes in planta. Long-distance transport and intercellular transport have been postulated. For these processes, the existence of cytokinin transporters has been suggested. Recently, a transporter loading the xylem (AtABCG14) and another for cellular import (AtPUP14) have been discovered. AtABCG14 participates in the xylem loading process of cytokinins and contributes to the positive regulation of shoot growth. The cellular importer AtPUP14 is required to suppress cytokinin signaling. A role of a transporter as stop signal is a new paradigm for a hormone transporter.114sciescopu

Pricing for Past Channel State Information in Multi-Channel Cognitive Radio Networks

Cognitive Radio (CR) networks have received significant attention as a promising approach to improve the spectrum efficiency of current license-based regulatory system. In CR networks, a Secondary User (SU) can use a spectrum vacancy that can be detected by either sensing-before-transmission or database access. However, it is often difficult to detect a vacant spectrum opportunity because of inaccuracies due to sensing and delays to update and/or the database that holds this information. In this paper, we develop a hybrid detection framework in multi-channel CR networks, where an SU can selectively sense a channel for spectrum vacancy by accessing the spectrum history of Markovian channels. We focus on the value of the channel history information offered by the Primary Provider (PP) of each channel, and consider a market for the information exchange between multiple PPs and SUs. We investigate the interplay between of the PPs and the SUs through their pricing and buying decisions for this information, in the presence of sensing inaccuracy, i.e., false alarm and miss detection