Mitochondrial apoptosis induced by Chamaemelum nobile extract in breast cancer cells

Chamaemelum nobile (Asteraceae) commonly known as ‹Roman chamomile› is a medicinal plant used for numerous diseases in traditional medicine, although its anticancer activity is unknown. The present study was carried out to investigate the anticancer as well as apoptotic activity of ethyl acetate fraction of C. nobile on different cancerous cell lines. The cells were treated with varying concentrations (0.001-0.25 mg/mL) of this fraction for 24, 48 and 72 h. Apoptosis induced in MCF-7 cells following treatment with ethyl acetate fraction was measured using Annexin V/PI, flowcytometry and western blotting analysis. The results showed that C. nobile ethyl acetate fraction revealed relatively high antiproliferative activity on MCF-7 cells; however, it caused minimal growth inhibitory response in normal cells. The involvement of apoptosis as a major cause of the fraction-induced cell death was confirmed by annexin-V/PI assay. In addition, ethyl acetate fraction triggered the mitochondrial apoptotic pathway by decreasing the Bcl-2 as well as increasing of Bax protein expressions and subsequently increasing Bax/Bcl-2 ratio. Furthermore, decreased proliferation of MCF-7 cells in the presence of the fraction was associated with G2/M phase cell cycle arrest. These findings confirm that ethyl acetate fraction of C.nobile may contain a diversity of phytochemicals which suppress the proliferation of MCF-7 cells by inducing apoptosis. © 2016 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services

EPIDEMIOLOGY OF VIRAL GASTROENTERITIS IN IRAN

Viruses are prominent causative agents of acute gastroenteritis in children <5 years of age per year.(1) In the present review, all viral gastroenteritis studies in Iran were assessed, and the mean prevalences of rotaviruses, noroviruses, enteric adenoviruses, sapoviruses and astroviruses associated with acute gastroenteritis were 39.9%, 6%, 5.7%, 4.2% and 2.7%, respectively. In 2 studies, human bocavirus and human parechovirus were detected in 21.8% and 23.7% of children with acute gastroenteritis, respectively

Analysis of the Role of Tortuosity and Infiltration Constants in the Beerkan Method

It has recently been proposed to couple the Beerkan method with the Beerkan Estimation of Soil Transfer parameters (BEST) algorithm to facilitate the estima- tion of soil hydraulic parameters from an infiltration experiment. Although this simplified field procedure is relatively rapid and inexpensive, it has been doubt - ed if the Beerkan method can represent a valid and reliable alternative to other conventional methods. This study explored the impact of the tortuosity param- eter (p) and two infiltration constants included in the BEST algorithm using a sensitivity analysis applied to three experimental soils. The analysis that was validated using the numerical model HYDRUS 2D/3D indicates that the tortuosity is relatively insignificant compared to parameters b and g that have a large impact on the estimation procedure

Intra-peritoneal and intra-rectal immunogenicity induced by rotavirus virus like particles 2/6/7 in mice

We previously developed virus like particles of rotavirus (RV) with VP2, VP6, and VP7 proteins (VLP2/6/7) using stable High-five cell line. To evaluate the immunogenicity of our construct, we assessed the humoral and cytokine responses induced by VLP2/6/7 in BALB/c mice immunized intra-peritoneally and intra-rectally. Enzyme-linked immunosorbent assay (ELISA) and Relative quantitative (RQ) Real-time PCR were used to evaluate the antibody (IgG and IgA) levels in serum and mRNA levels of IL-6, IL-10 and IFN-gamma in spleen cells, respectively. Our results showed that VLP2/6/7 is capable of intra-peritoneal (I.P.) and intra-rectal (I.R.) induction of serum IgG and IgA responses. IgA was detected in fecal samples of immunization groups by I.P. and I.R. routes. Interestingly, I.R. route induced higher IgA titer compared with I.P. route which was statistically significant. Moreover, mRNA levels of IL-6 and IFN-gamma were significantly elevated in mice immunized intra- peritoneally with VLP2/6/7 compared to control group. As such, the mean change was 7.4 (P < 0.05) and 14.8 (P < 0.001) for IFN-gamma and IL-6, respectively. Likewise, the same pattern was found when mice were immunized intra-rectally. Although elevated, the difference in the mean change for IL-10 was not statistically significant when compared to control group. Our findings indicated that VLPs constructed via a stable insect cell line are able to induce both humoral and cellular responses, a similar pattern as observed after immunization with live RVs. (C) 2014 Elsevier Ltd. All rights reserved

Efficient inhibition of human immunodeficiency virus replication using novel modified microRNA-30a targeting 3'-untranslated region transcripts

RNA interference (RNAi)-based gene therapy is currently considered to be a combinatorial anti-human immunodeficiency virus-1 (HIV-1) therapy. Although arti­ficial polycistronic microRNAs (miRs) can reduce HIV-1 escape mutant variants, this approach may increase the risk of side effects. The present study aimed to optimize the efficiency of anti-HIV RNAi gene therapy in order to reduce the cell toxicity induced by multi-short hairpin RNA expression. An artificial miR-30a-3'-untranslated region (miR-3'-UTR) obtained from a single RNA polymerase II was used to simultaneously target all viral transcripts. The results of the present study demonstrated that HIV-1 replication was signifi­cantly inhibited in the cells with the miR-3'-UTR construct, suggesting that miR-3'-UTR may serve as a promising tool for RNAi-based gene therapy in the treatment of HIV-1. © 2016, Spandidos Publications. All Rights Reserved

Comparison of numerical, analytical, and empirical models to estimate wetting patterns for surface and subsurface drip irrigation

Due to increasing competition for water resources by urban, industrial, and agricultural users, the proportion of agricultural water use is gradually decreasing. To maintain or increase agricultural production, new irrigation systems, such as surface or subsurface drip irrigation systems, will need to provide higher water use efficiency than those traditionally used. Several models have been developed to predict the dimensions of wetting patterns, which are important to design optimal drip irrigation system, using variables such as the emitter discharge, the volume of applied water, and the soil hydraulic properties. In this work, we evaluated the accuracy of several approaches used to estimate wetting zone dimensions by comparing their predictions with field and laboratory data, including the numerical HYDRUS-2D model, the analytical WetUp software, and selected empirical models. The soil hydraulic parameters for the HYDRUS-2D simulations were estimated using either Rosetta for the laboratory experiments and inverse analysis for the field experiments. The mean absolute error (MAE) was used to compare the model predictions and observations of wetting zone dimensions. MAE for different experiments and directions varied from 0.87 to 10.43 cm for HYDRUS-2D, from 1 to 58.1 cm for WetUp, and from 1.34 to 12.24 cm for other empirical models

Numerical simulations of water movement in a subsurface drip irrigation system under field and laboratory conditions using HYDRUS-2D

a b s t r a c t Due to the decreasing availability of water resources and the increasing competition for water between residential, industrial, and agricultural users, increasing irrigation efficiency, by methods like subsurface drip irrigation (SDI) systems, is a pressing concern for agricultural authorities. To properly manage SDI systems, and increase the efficiency of the water/fertilizer use while reducing water losses due to evaporation, the precise distribution of water around the emitters must be known. In this paper, the Windows-based computer software package HYDRUS-2D, which numerically simulates water, heat, and/or solute movement in two-dimensional, variably-saturated porous media, was used to evaluate the distribution of water around the emitter in a clay loam soil. The simulation results were compared with two sets of laboratory and field experiments involving SDI with emitters installed at different depths, and were evaluated using the root-mean-square-error (RMSE). The RMSE at different locations varied between 0.011 and 0.045 for volumetric water contents, and between 0.98 and 4.36 cm for wetting dimensions. Based on these values, it can be concluded that the correspondence between simulations and observations was very good

Numerical simulations of water movement in a subsurface drip irrigation system under field and laboratory conditions using HYDRUS-2D

Due to the decreasing availability of water resources and the increasing competition for water between residential, industrial, and agricultural users, increasing irrigation efficiency, by methods like subsurface drip irrigation (SDI) systems, is a pressing concern for agricultural authorities. To properly manage SDI systems, and increase the efficiency of the water/fertilizer use while reducing water losses due to evaporation, the precise distribution of water around the emitters must be known. In this paper, the Windows-based computer software package HYDRUS-2D, which numerically simulates water, heat, and/or solute movement in two-dimensional, variably-saturated porous media, was used to evaluate the distribution of water around the emitter in a clay loam soil. The simulation results were compared with two sets of laboratory and field experiments involving SDI with emitters installed at different depths, and were evaluated using the root-mean-square-error (RMSE). The RMSE at different locations varied between 0.011 and 0.045 for volumetric water contents, and between 0.98 and 4.36cm for wetting dimensions. Based on these values, it can be concluded that the correspondence between simulations and observations was very good.Subsurface drip irrigation HYDRUS-2D Wetting dimensions