Synthesis and cytotoxic activity of a novel dihydroisoquinoline-derived hydroxamic acid

Naturally occurring hydroxamic acid derivatives are biosynthesized by microorganisms (siderophores) and plants (benzoxazinoids). Recent developments in drug related research have highlighted the promising biological and pharmacological properties that the hydroxamic acid function may offer for the enhancement of therapeutic applications. This study reports on the full synthesis of a new dihydroisoquinoline hydroxamic acid (2-Hydroxy-3,3-dimethyl-7-nitro-3,4-dihydroisoquinolin-1(2H)-one). It also describes its cytotoxicity with regard to the human hepatocarcinoma cell line Hep3B.    Â

2-Hydr­oxy-3,3-dimethyl-7-nitro-3,4-dihydro­isoquinolin-1(2H)-one

In the title compound, C11H12N2O4, a new hydroxamic acid which belonging to the isoquinole family, the heterocyclic ring adopts a half-chair conformation. The nitro group is essentially coplanar with the aromatic ring. Inter­molecular O—H⋯O hydrogen bonds assemble the mol­ecules around inversion centres to form pseudo-dimers

Identification of candidate regions for a novel Usher syndrome type II locus

PURPOSE: Chronic diseases affecting the inner ear and the retina cause severe impairments to our communication systems. In more than half of the cases, Usher syndrome (USH) is the origin of these double defects. Patients with USH type II (USH2) have retinitis pigmentosa (RP) that develops during puberty, moderate to severe hearing impairment with downsloping pure-tone audiogram, and normal vestibular function. Four loci and three genes are known for USH2. In this study, we proposed to localize the gene responsible for USH2 in a consanguineous family of Tunisian origin. METHODS: Affected members underwent detailed ocular and audiologic characterization. One Tunisian family with USH2 and 45 healthy controls unrelated to the family were recruited. Two affected and six unaffected family members attended our study. DNA samples of eight family members were genotyped with polymorphic markers. Two-point and multipoint LOD scores were calculated using Genehunter software v2.1. Sequencing was used to investigate candidate genes. RESULTS: Haplotype analysis showed no significant linkage to any known USH gene or locus. A genome-wide screen, using microsatellite markers, was performed, allowing the identification of three homozygous regions in chromosomes 2, 4, and 15. We further confirmed and refined these three regions using microsatellite and single-nucleotide polymorphisms. With recessive mode of inheritance, the highest multipoint LOD score of 1.765 was identified for the candidate regions on chromosomes 4 and 15. The chromosome 15 locus is large (55 Mb), underscoring the limited number of meioses in the consanguineous pedigree. Moreover, the linked, homozygous chromosome 15q alleles, unlike those of the chromosome 2 and 4 loci, are infrequent in the local population. Thus, the data strongly suggest that the novel locus for USH2 is likely to reside on 15q. CONCLUSIONS: Our data provide a basis for the localization and the identification of a novel gene implicated in USH2, most likely localized on 15q

An efficient and unified 2D-inverse integer cosine transform (IICT) FPGA-hardware implementation for HEVC standard

International audienc

An optimized and unified architecture design for H.265/HEVC 1-D inverse core transform

International audienc

AN EFFICIENT ARCHITECTURE VLSI FOR 4×4 INTRA PREDICTION IN HEVC STANDARD

International audienceThe HEVC is a proposal of new video coding standard that will be used for a wide range of applications like ULTRA HD and 3D applications. MPEG and VCEG have established a Joint Collaborative Team on Video Coding (JCT-VC) to develop the HEVC (High Efficiency Video Coding) standard which is expected to provide a significant improvement in data transmission and streaming efficiency compared to H.264. In this proposal standard, various modules of coding are defined. Among the most complex is the module of the intra prediction. The HEVC defines 35 modes of intra prediction for 8 × 8, 16 × 16, 32 × 32, 3 modes for 64 × 64 and 17 modes for 4 × 4 while the H.264/AVC (Advanced Video Coding) uses 9 modes for intra 4 × 4 and 4 modes for intra 16 × 16. In this paper, we propose an efficient uniform architecture for all of the 4 × 4 intra directional modes. This architecture offers an important gain in case of treatment time compared to the literature. Our proposed architecture is implemented with the technology TSMC 0.18μm CMO

Identification of Alternaria Species Recovered from Stored Durum Wheat Kernels in Tunisia

This study confirms the wheat natural infection by Alternaria species associated with black point disease of stored kernels. These species are able to produce plant mycotoxins as well as toxic metabolites, which may have consequences in the food industry and agriculture as well as in health services. Because of the tight link between fungal species and metabolite production, correct identification of the mould at the species level is required. For this purpose, morphological characters and molecular techniques based on the amplification and sequencing of the ITS1-5.8S-ITS2 region of the rDNA were used. The analysis identified six species of Alternaria, namely A. alternata, A. tenuissima, A. arborescens, A. mali, A. longipes, and A. brassicae. The most frequently isolated species were A. alternata and A. tenuissima with an overall prevalence of 36.1 and 30.6%, respectively

Histopathological, oxidative damage, biochemical and genotoxicity alterations in hepatic rats exposed to deltamethrin: modulatory effects of garlic (Allium sativum)

Deltamethrin is a pesticide widely used as a synthetic pyrethroid. The aim of this study was undertaken to investigate the effects of deltamethrin to induce oxidative stress and changes in biochemical parameters, hepatotoxicity and genotoxicity in female rats following a short-term (30 days) oral exposure and attenuation of these effects by Allium sativum extract. Indeed, Allium sativum is known to be a good antioxidant food resource which helps destroy free radicals particles. Our results showed that deltamethrin treatment caused an increase in liver enzyme activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH); and hepatic lipid peroxidation (LPO) level. However, it induced a decrease in activities of hepatic catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) (pThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author