Эффективность использования бюджетных средств в реализации функции МЧС (на примере ГУ МЧС России по г. Севастополю)

В выпускной квалификационной работе была проанализирована эффективность использования бюджетных средств (на примере ГУ МЧС России по г. Севастополю). Разработаны рекомендации относительно изменения состава анализа бюджетирования.In the final qualifying work, the effectiveness of the use of budgetary funds was analyzed (on the example of the Main Directorate of the Ministry of Emergency Situations of Russia for the city of Sevastopol). Recommendations have been developed for changing the composition of the budgeting analysis

Проект установки водоподготовки в производстве метанола ООО «Сибметахим"

Объект исследования является система водоснабжения ООО «Сибметахим» г. Томск, которая включает очистку от взвешенных частиц, получение деминерализованной воды и турбинных конденсатов. Разрабатываемый проект стадии водоподготовки представляет собой комплекс сооружений, оборудования и трубопроводов обеспечивающих забор воды из реки Томь, очистку и ее обработку, транспортирование и подачу воды потребителю требуемых расходов и качества. Цель дипломного проекта является разработка усовершенствованной схемы очистки речной воды. Технологическая схема водоподготовительной установки включает механическую и физико-химическую очистку сточной воды. Основным аппаратом является ионообменные фильтры, размер и количество аппаратов было определенно исходя из материального баланса и аппаратурного расчета. Так же были проведены расчеты теплового баланса, гидравлического расчета, механического расчета. Экономическими показателями данного водоподготовительного цеха позволяет покрыть расходы на модернизацию в течении 95 месяцев.The object of study is the water supply system, OOO Sibmetahim" (Tomsk), which includes purification from suspended particles, obtaining demineralized water and turbine condensates. The project stage of water treatment is a complex of structures, equipment and pipelines to ensure water intake from the Tom river, cleaning and processing, transport and water supply of the required costs and quality. The goal of the graduation project is to develop an improved purification of river water. Technological scheme of water treatment plant includes the mechanical and physico-chemical treatment of waste water

Biological activity of extracts from some Mediterranean Macrophytes

5 páginas, 1 tablaSeventy one species of marine macrophytes from the Central Mediterranean have been screened for the production of antibacterial, antifungal, antiviral, cytotoxic and antimitotic compounds. Sixty five of the species displayed some kind of activity and most of them were active on more than one organism or cell tested. Antifungal activity was the most widespread (70% of the plants), whilst the incidence of antibacterial activity was extraordinarily low (6% of the plants). Of the plants tested 21% showed antiviral activity, 35% were cytotoxic and nearly 50% had antimitotic properties. The maximum level of activity was found among the Chlorophyfa; some members of the Bryopsidales (Flabellia petiolata, Caulerpa prolifera, Halimeda tuna) were the most active species. Most of the dominant species in Mediterranean phytobenthic communities (Corallina elongata, Lithophyllurn lichenoides, Phyllophora crispa, Cystoseira spp., Halopteris spp., Codium spp., Halimeda tuna, Valonia utricularis, Posidonia oceanica, Zostera noltii and Cyrnodocea nodosa) exhibited strong antifungal properties.This study was supported by the projects ECOFARM and CICYT MAR91-0528.Peer reviewe

Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations

Using isolated rat cardiomyocytes we have examined: 1) the effect of insulin on the cellular distribution of glucose transporter 4 (GLUT4) and GLUT1, 2) the total amount of these transporters, and 3) the co-localization of GLUT4, GLUT1, and secretory carrier membrane proteins (SCAMPs) in intracellular membranes. Insulin induced 5.7- and 2.7-fold increases in GLUT4 and GLUT1 at the cell surface, respectively, as determined by the nonpermeant photoaffinity label [3H]2-N-[4(1-azi-2,2,2-trifluoroethyl)benzoyl]-1, 3-bis-(D-mannos-4-yloxy)propyl-2-amine. The total amount of GLUT1, as determined by quantitative Western blot analysis of cell homogenates, was found to represent a substantial fraction ( approximately 30%) of the total glucose transporter content. Intracellular GLUT4-containing vesicles were immunoisolated from low density microsomes by using monoclonal anti-GLUT4 (1F8) or anti-SCAMP antibodies (3F8) coupled to either agarose or acrylamide. With these different immunoisolation conditions two GLUT4 membrane pools were found in nonstimulated cells: one pool with a high proportion of GLUT4 and a low content in GLUT1 and SCAMP 39 (pool 1) and a second GLUT4 pool with a high content of GLUT1 and SCAMP 39 (pool 2). The existence of pool 1 was confirmed by immunotitration of intracellular GLUT4 membranes with 1F8-acrylamide. Acute insulin treatment caused the depletion of GLUT4 in both pools and of GLUT1 and SCAMP 39 in pool 2. In conclusion: 1) GLUT4 is the major glucose transporter to be recruited to the surface of cardiomyocytes in response to insulin; 2) these cells express a high level of GLUT1; and 3) intracellular GLUT4-containing vesicles consist of at least two populations, which is compatible with recently proposed models of GLUT4 trafficking in adipocytes