40 research outputs found
Biomolecular composition of porcine ovarian follicles following in vitro treatment of vitamin and insulin alone or in combination
The impact of migration on polish nurses : pilot study
Abstract
Introduction. Migration of professional nurses is a global phenomenon. Polish nurses usually migrate to the relatively richer countries, especially in Europe, thanks to the opening of the borders of the European Union (2004) and legal regulations in the field of education of nurses (Directive 2005/36/EC). Aim. The analysis of symptoms and determinants of acculturative stress and acculturative strategies among Polish migrant nurses working in the UK. Material and methods. The study used a proprietary questionnaire and standardized tool the East Asian Acculturation Measure - Scale of Acculturative Strategy. The research was carried out through Internet among 62 people. Statistical analysis was performed using Mann-Whitney test, Kruskal-Wallis test, Dunn test, coefficient Spearman. Results. The main reason for the migration of respondents to the UK was the desire to improve financial situation and professional development. Responders found job as a nurse, including a charge nurse and managers in hospitals and nursing homes. Acculturative stress manifested most often in social functioning, then in the emotional, cognitive and physical aspects. It was characterized by a sense of lack of interest from British people, sadness, feelings of loneliness, lower self-confidence and shyness. It was determined significantly by the age of the respondents, the length of stay in the UK, knowledge of English and maintaining social contacts with the British. The most commonly used acculturation strategy was integration, conditioned by the length of stay in the UK and symptoms of acculturative stress. Conclusions. Working in a foreign country is associated with acculturative stress, implementation of acculturation adaptive and non-adaptive strategies.</jats:p
Alterations in the antioxidant enzyme activities in the neurodevelopmental rat model of schizophrenia induced by glutathione deficiency during early postnatal life
The aim of the present study was to assess the e ects of l-buthionine-(S,R)-sulfoximine
(BSO), a glutathione (GSH) synthesis inhibitor, and GBR 12909, a dopamine reuptake inhibitor,
administered alone or in combination to Sprague-Dawley rats during early postnatal development
(p5–p16), on the levels of reactive oxygen species (ROS), lipid peroxidation (LP) and the activities of
antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and
glutathione disulfide reductase (GR) in peripheral tissues (liver, kidney) and selected brain structures
(prefrontal cortex, PFC; hippocampus, HIP; and striatum, STR) of 16-day-old rats. The studied
parameters were analyzed with reference to the content of GSH and sulfur amino acids, methionine
(Met) and cysteine (Cys) described in our previous study. This analysis showed that treatment with a
BSO + GBR 12909 combination caused significant decreases in the lipid peroxidation levels in the
PFC and HIP, in spite of there being no changes in ROS. The reduction of lipid peroxidation indicates
a weakening of the oxidative power of the cells, and a shift in balance in favor of reducing processes.
Such changes in cellular redox signaling in the PFC and HIP during early postnatal development
may result in functional changes in adulthood
Effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the rabbit ovary - an in vivo study
Glutathione deficiency and alterations in the sulfur amino acid homeostasis during early postnatal development as potential triggering factors for schizophrenia-like behavior in adult rats
Impaired glutathione (GSH) synthesis and dopaminergic transmission are important
factors in the pathophysiology of schizophrenia. Our research aimed to assess the effects of
l-buthionine-(S,R)-sulfoximine (BSO), a GSH synthesis inhibitor, and GBR 12909, a dopamine
reuptake inhibitor, administered alone or in combination, to Sprague-Dawley rats during early
postnatal development (p5-p16), on the levels of GSH, sulfur amino acids, global DNA methylation,
and schizophrenia-like behavior. GSH, methionine (Met), homocysteine (Hcy), and cysteine (Cys)
contents were determined in the liver, kidney, and in the prefrontal cortex (PFC) and hippocampus
(HIP) of 16-day-old rats. DNA methylation in the PFC and HIP and schizophrenia-like behavior
were assessed in adulthood (p90-p93). BSO caused the tissue-dependent decreases in GSH content
and alterations in Met, Hcy, and Cys levels in the peripheral tissues and in the PFC and HIP.
The changes in these parameters were accompanied by alterations in the global DNA methylation in
the studied brain structures. Parallel to changes in the global DNA methylation, deficits in the social
behaviors and cognitive functions were observed in adulthood. Only BSO + GBR 12909-treated rats
exhibited behavioral alterations resembling positive symptoms in schizophrenia patients. Our results
suggest the usefulness of this neurodevelopmental model for research on the pathomechanism
of schizophrenia
Effects of L-DOPA on Gene Expression in the Frontal Cortex of Rats with Unilateral Lesions of Midbrain Dopaminergic Neurons
Evaluation of cysteine metabolism in the rat liver and kidney following intravenous cocaine administration and abstinence
Many toxic effects of cocaine are attributed to reactive oxygen species (ROS) generated during its metabolism. Recently, it has been suggested that the biological action of ROS is often confused with endogenously generated reactive sulfur species (RSS). The aim of this study was to evaluate the impact of cocaine on thiols and RSS in the rat liver and kidney in the drug self-administration (SA) paradigm and the cocaine yoked delivery model (YC) followed by drug abstinence with extinction training. The level of thiols as well as RSS formed during anaerobic metabolism of cysteine and sulfate were assayed. In addition, the activity of enzymes involved in RSS formation and glutathione metabolism were determined. In the liver, following direct cocaine administration (SA and YC), the RSS levels decreased, while in the kidneys, cocaine increased the RSS contents in both groups. These changes were maintained in these tissues during drug abstinence. The level of sulfates was changed by cocaine only in the liver. In the kidney, cocaine shifted cysteine metabolism towards an anaerobic pathway. Our study demonstrates for the first time the changes in cysteine metabolism and thiol levels in the liver and kidney of rats after cocaine self-administration and abstinence
Studies on the vitrified and cryomilled bosentan
In this paper, several experimental techniques [X-ray diffraction,
differential scanning calorimetry (DSC), thermogravimetry, Fourier transform infrared
spectroscopy, and broad-band dielectric spectroscopy] have been applied to
characterize the structural and thermal properties, H-bonding pattern, and molecular
dynamics of amorphous bosentan (BOS) obtained by vitrification and cryomilling of
the monohydrate crystalline form of this drug. Samples prepared by these two methods
were found to be similar with regard to their internal structure, H-bonding scheme, and
structural (α) dynamics in the supercooled liquid state. However, based on the analysis
of α-relaxation times (dielectric measurements) predicted for temperatures below the
glass-transition temperature (Tg), as well as DSC thermograms, it was concluded that
the cryoground sample is more aged (and probably more physically stable) compared
to the vitrified one. Interestingly, such differences in physical properties turned out to
be reflected in the lower intrinsic dissolution rate of BOS obtained by cryomilling (in
the first 15 min of dissolution test) in comparison to the vitrified drug. Furthermore, we showed that cryogrinding of the crystalline
BOS monohydrate leads to the formation of a nearly anhydrous amorphous sample. This finding, different from that reported by
Megarry et al. [Carbohydr. Res. 2011, 346, 1061−1064] for trehalose (TRE), was revealed on the basis of infrared and thermal
measurements. Finally, two various hypotheses explaining water removal upon cryomilling have been discussed in the manuscript
Argumentacja w edukacji: postulaty badań edukacyjnych w polskiej szkole argumentacji
Uwzględniając dotychczasową działalność Polskiej Szkoły Argumentacji,
w szczególności jej działalność edukacyjną wyeksponowaną
podczas XV konferencji ArgDiaP, sformułowaliśmy postulaty dotyczące
dalszej działalności edukacyjnej Szkoły. Ich realizacja w ciągu
najbliższych lat mogłaby stanowić grunt dla długoterminowych celów
edukacyjnych, takich jak (1) opracowanie spójnego programu nauczania
sztuki argumentowania i krytycznego myślenia w szkołach podstawowych
i średnich oraz (2) zaprojektowanie ogólnopolskich standardów
i ram nauczania przedmiotów powiązanych z argumentacją
i krytycznym myśleniem na poziomie studiów uniwersyteckich